Large-scale fabrication of free-standing and sub-μm PDMS through-hole membranes

Le‐The, Hai; Tibbe, Martijn Peter; Loessberg-Zahl, Joshua; Carmo, Marciano Palma do; Helm, Marinke van der; Bomer, Johan G.; Berg, Albert van den; Leferink, Anne Marijke; Segerink, Loes; Eijkel, Jan C.T.

doi:10.1039/c7nr09658e

Cited by 39 publications

(47 citation statements)

References 37 publications

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“…This membrane can be integrated into the multiplexed chip without additional glue. The fabrication process was improved from our previous work 35 so that it requires only one step of photoresist (PR) spin-coating ( Fig. 2).…”

Section: Membrane Fabricationmentioning

confidence: 99%

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Multiplexed blood–brain barrier organ-on-chip

et al. 2020

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Section: Membrane Fabricationmentioning

confidence: 99%

“…Next, the PDMS solution was spin-coated over the fabricated PR column arrays at 4000 rpm for 1 min and baked in the oven at 60°C for at least 3 h. When spincoating PDMS, it can fully cover the PR columns. 35 To make sure that the pores are open, it is necessary to perform a plasma etching process of the cured PDMS membrane.…”

Section: Membrane Fabricationmentioning

confidence: 99%

Multiplexed blood–brain barrier organ-on-chip

et al. 2020

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“…Based on the outstanding advantages of PDMS, such as non-toxicity, high hydrophobicity, chemical resistance, gas permeability, optical transparency, environmental friendliness that does not bio-accumulate, flexibility, low costs, and good molding capability [34,115], through-hole PDMS membranes found wide applications in the biomedical and chemical fields, such as sterile filtration, cell sorting, biomolecular separation, termed organs-on-a-chip systems, microfluidic devices, thin film extraction, lab-on-a-chip devices, micro total analysis systems, and permeation passive samplers [34,115,116,117,118].…”

Section: Pdms Membranesmentioning

confidence: 99%

A Review on Porous Polymeric Membrane Preparation. Part II: Production Techniques with Polyethylene, Polydimethylsiloxane, Polypropylene, Polyimide, and Polytetrafluoroethylene

2019

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The development of porous polymeric membranes is an important area of application in separation technology. This article summarizes the development of porous polymers from the perspectives of materials and methods for membrane production. Polymers such as polyethylene, polydimethylsiloxane, polypropylene, polyimide, and polytetrafluoroethylene are reviewed due to their outstanding thermal stability, chemical resistance, mechanical strength, and low cost. Six different methods for membrane fabrication are critically reviewed, including thermally induced phase separation, melt-spinning and cold-stretching, phase separation micromolding, imprinting/soft molding, manual punching, and three-dimensional printing. Each method is described in details related to the strategy used to produce the porous polymeric membranes with a specific morphology and separation performances. The key factors associated with each method are presented, including solvent/non-solvent system type and composition, polymer solution composition and concentration, processing parameters, and ambient conditions. Current challenges are also described, leading to future development and innovation to improve these membranes in terms of materials, fabrication equipment, and possible modifications.

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“…More recently, Boyden chamber-type culture has been combined with microfluidics in the development of vascularized organs-on-chips, in which endothelial cells are cultured on the opposite side of an intermediate porous membrane from parenchymal cells on a single microdevice to recreate an important bloodtissue interface [81]. Organ-on-chip development has been facilitated by novel manufacturing techniques to create thin, porous membranes [82]. In alternative designs, gates or openings have been micromanufactured across PDMS membranes to connect parallel adjacent channels and let co-cultured vascular and parenchymal cells interact [83,84].…”

Section: 5d Culture Modelsmentioning

confidence: 99%

Biofabrication strategies for creating microvascular complexity

2019

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Design and fabrication of effective biomimetic vasculatures constitutes a relevant and yet unsolved challenge, lying at the heart of tissue repair and regeneration strategies. Even if cell growth is achieved in 3D tissue scaffolds or advanced implants, tissue viability inevitably requires vascularization, as diffusion can only transport nutrients and eliminate debris within a few hundred microns. This engineered vasculature may need to mimic the intricate branching geometry of native microvasculature, referred to herein as vascular complexity, to efficiently deliver blood and recreate critical interactions between the vascular and perivascular cells as well as parenchymal tissues. This review first describes the importance of vascular complexity in labs-and organs-on-chips, the biomechanical and biochemical signals needed to create and maintain a complex vasculature, and the limitations of current 2D, 2.5D, and 3D culture systems in recreating vascular complexity. We then critically review available strategies for design and biofabrication of complex vasculatures in cell culture platforms, labs-and organs-on-chips, and tissue engineering scaffolds, highlighting their advantages and disadvantages. Finally, challenges and future directions are outlined with the hope of inspiring researchers to create the reliable, efficient and sustainable tools needed for design and biofabrication of complex vasculatures.

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Large-scale fabrication of free-standing and sub-μm PDMS through-hole membranes

Abstract: A robust and simple method was developed for large-scale fabrication of free-standing and sub-μm PDMS through-hole membranes for biomedical applications.

Cited by 39 publications

References 37 publications

Multiplexed blood–brain barrier organ-on-chip

Multiplexed blood–brain barrier organ-on-chip

A Review on Porous Polymeric Membrane Preparation. Part II: Production Techniques with Polyethylene, Polydimethylsiloxane, Polypropylene, Polyimide, and Polytetrafluoroethylene

Biofabrication strategies for creating microvascular complexity

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