Large‐scale gene expression profiling data for the model moss Physcomitrella patens aid understanding of developmental progression, culture and stress conditions

Abstract

SUMMARYThe moss Physcomitrella patens is an important model organism for studying plant evolution, development, physiology and biotechnology. Here we have generated microarray gene expression data covering the principal developmental stages, culture forms and some environmental/stress conditions. Example analyses of developmental stages and growth conditions as well as abiotic stress treatments demonstrate that (i) growth stage is dominant over culture conditions, (ii) liquid culture is not stressful for the plant, (iii) low pH might aid protoplastation by reduced expression of cell wall structure genes, (iv) largely the same gene pool mediates response to dehydration and rehydration, and (v) AP2/EREBP transcription factors play important roles in stress response reactions. With regard to the AP2 gene family, phylogenetic analysis and comparison with Arabidopsis thaliana shows commonalities as well as uniquely expressed family members under drought, light perturbations and protoplastation. Gene expression profiles for P. patens are available for the scientific community via the easy-to-use tool at https://www.genevestigator.com. By providing large-scale expression profiles, the usability of this model organism is further enhanced, for example by enabling selection of control genes for quantitative real-time PCR. Now, gene expression levels across a broad range of conditions can be accessed online for P. patens.

Sporophyte formation and life cycle completion in moss requires heterotrimeric G-proteins

“…While the morphological details of the moss sporophyte, its developmental time line, and the transcriptional profile are relatively well described (Cove et al, 2009;Vidali and Bezanilla, 2012;Hiss et al, 2014;Scanlon, 2015a, 2015b;Ortiz-Ramirez et al, 2016), the mechanistic details and signal transduction pathways involved during sporophyte formation are largely unknown. Our results provide a clue to the types of proteins that might be involved in the signaling event that takes place during sporophyte formation.…”

Can mosses serve as model organisms for forest research?

“…Results include the avoidance of stress by downregulation of photosynthesis (Cuming et al 2007;Hiss et al 2014) and the rapid upregulation of protective mechanism for photosynthesis, membranes and protein folding (Azzabi et al 2012;Hiss et al 2014;Ruibal et al 2013). At the metabolic level, free soluble sugars, such as trehanderose, are accumulated to increase the intracellular osmolarity and counterbalance water-loss (Nagao et al 2005(Nagao et al , 2006.…”

“…To date, most attention has been paid to the mechanisms by which mosses master the cellular challenges during dehydration/rehydration and freezing, sometimes also employing ABA or salt treatment (Cui et al 2012;Cuming et al 2007;Hiss et al 2014;Nagao et al 2005;Oliver et al 2005). Results include the avoidance of stress by downregulation of photosynthesis (Cuming et al 2007;Hiss et al 2014) and the rapid upregulation of protective mechanism for photosynthesis, membranes and protein folding (Azzabi et al 2012;Hiss et al 2014;Ruibal et al 2013).…”

“…In recent years, several -omics techniques have added a considerable amount of new data concerning the P. patens metabolome and proteome (Erxleben et al 2012;Mueller et al 2014), as well as the transcriptome (Beike et al 2015b;Cuming et al 2007;O'Donoghue et al 2013;Richardt et al 2010), which can be screened on the Genevestigator transcriptomics platform (Hiss et al 2014).…”

Identification of Targets and Interaction Partners of Arginyl-tRNA Protein Transferase in the Moss Physcomitrella patens

“…Further, the axenic cultivation of different growth states under highly standardized conditions facilitates reproducible high throughput analysis of proteomes, transcriptomes, and metabolites (35)(36)(37)(38)(39)(40)(41). Recently, we reported that ATE abundance underlies spatiotemporal patterning in the moss Physcomitrella patens using a translational GUS reporter fusion via knock-in at the endogenous genomic locus (11).…”