Bioseparation
DOI: 10.1007/bfb0046196
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Large-scale gradient elution chromatography

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Cited by 8 publications
(6 citation statements)
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“…Gradient elution was performed as sharp peaks are obtained within short separation time [ 30 ]. The standard β-ODAP was not chemically modified and resolved into peak with retention time of about 7.4 min.…”
Section: Resultsmentioning
confidence: 99%
“…Gradient elution was performed as sharp peaks are obtained within short separation time [ 30 ]. The standard β-ODAP was not chemically modified and resolved into peak with retention time of about 7.4 min.…”
Section: Resultsmentioning
confidence: 99%
“…Lipophilic species are mostly neutral with hydrophobic properties, owing to the alkyl groups . Chromatographic approaches, like ion-pairing reversed-phase, ion-exchange, ion-exclusion, and reversed-phase chromatographies, are reported to facilitate speciation of arsenicals in marine sample extracts, owing to variabilities in their physicochemical properties. , For example, at pH 7, MMA at p K a = 2.6 and DMA at p K a = 6.1 are anions and arsenocholine (AsC), tetramethylarsonium ion (TETRA), and trimethylarsine oxide (TMAO) at p K a = 3.6 are cations, while arsenobetaine (AsB) at p K a = 2.18 is zwitterionic. , Methylated arsenicals and AsSugars have been successfully isolated on anion-exchange (AE) columns, , while cation-exchange (CE) columns provide effective isolations for AsB, AsC, DMA, TMAO, TETRA, and DMAA. , AsLipids, however, require additional separation, using RP-LC typically with a C8 or C18 column. ,, …”
Section: Separationmentioning
confidence: 99%
“…Ordinarily, AE is performed in isocratic mode, mainly using aqueous mobile phases consisting of buffer salts with pH ranges covering neutral, basic, and acidic conditions . Gradient elution can shorten the analysis time without compromising resolutions of target analytes, allows for a broad range of retention, has a high peak capacity capable of handling a complex matrix, like seafood, and can overcome the general issues associated with elution. , Gradient elution protocols are rarely used because they cause baseline instabilities that could lead to inaccurate quantification and generally require extra instrument maintenance in comparison to isocratic elution. , …”
Section: Separationmentioning
confidence: 99%
“…1-2.4. 11.3 Evaluations of PeLi, 11i, and Bii 117 Truei et al [113] used some of the correlations above for parameter estimations in a case study involving gradient elution of four proteins in hydrophobic interaction chromatography. d is the molecular diameter of the solute.…”
mentioning
confidence: 99%