Large Scale Hippocampal Cellular Distress may Explain the Behavioral Consequences of Repetitive Traumatic Experiences-A Proteomic Approach

Uys, Joachim D.; Hattingh, S.; Stein, Dan J.; Daniels, Willie M. U.

doi:10.1007/s11064-008-9615-y

Cited by 8 publications

(4 citation statements)

References 66 publications

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“…Notably similar categories of proteins have been found to differ in their expression level following maternal separation [8], [9], chronic stress [50], [51], [52] and in the schizophrenic human brain [53], [54]. It should be noted that in our study, as in other similar ones using proteomic analysis based on 2-D gels [30], [55], there is a bias towards abundant, soluble proteins, with intermediate molecular weights.…”

Section: Discussionsupporting

confidence: 67%

Effects of an Early Experience of Reward through Maternal Contact or its Denial on Laterality of Protein Expression in the Developing Rat Hippocampus

et al. 2012

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Laterality is a basic characteristic of the brain which is detectable early in life. Although early experiences affect laterality of the mature brain, there are no reports on their immediate neurochemical effects during neonatal life, which could provide evidence as to the mechanisms leading to the lateralized brain. In order to address this issue, we determined the differential protein expression profile of the left and right hippocampus of 13-day-old rat control (CTR) pups, as well as following exposure to an early experience involving either receipt (RER) or denial (DER) of the expected reward of maternal contact. Proteomic analysis was performed by 2-dimensional polyacrylamide gel electrophoresis (PAGE) followed by mass spectroscopy. The majority of proteins found to be differentially expressed either between the three experimental groups (DER, RER, CTR) or between the left and right hemisphere were cytoskeletal (34%), enzymes of energy metabolism (32%), and heat shock proteins (17%). In all three groups more proteins were up-regulated in the left compared to the right hippocampus. Tubulins were found to be most often up-regulated, always in the left hippocampus. The differential expression of β-tubulin, β-actin, dihydropyrimidinase like protein 1, glial fibrillary acidic protein (GFAP) and Heat Shock protein 70 revealed by the proteomic analysis was in general confirmed by Western blots. Exposure to the early experience affected brain asymmetry: In the RER pups the ratio of proteins up-regulated in the left hippocampus to those in the right was 1.8, while the respective ratio was 3.6 in the CTR and 3.4 in the DER. Our results could contribute to the elucidation of the cellular mechanisms mediating the effects of early experiences on the vulnerability for psychopathology, since proteins shown in our study to be differentially expressed (e.g. tubulins, dihydropyrimidinase like proteins, 14-3-3 protein, GFAP, ATP synthase, α-internexin) have also been identified in proteomic analyses of post-mortem brains from psychiatric patients.

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Section: Discussionsupporting

confidence: 67%

Effects of an Early Experience of Reward through Maternal Contact or its Denial on Laterality of Protein Expression in the Developing Rat Hippocampus

et al. 2012

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“…However, an important unanswered question is the possible impact of chronic emotional stress during adulthood in animals that experienced stress during adolescence. In this regard, important neuroplasticity has been reported in the limbic systems of adult animals that were stressed during adolescence (Leussis et al, 2008;Toth et al, 2008;Uys et al, 2008Uys et al, , 2006Watt et al, 2009), which can affect the ability to cope with stress in adulthood. Indeed, studies have reported that stressful events in adulthood evoked greater behavioral changes in animals with a history of stress during adolescence (Avital and Richter-Levin, 2005;Tsoory et al, 2007).…”

Section: Perspective For Future Researchmentioning

confidence: 99%

Adolescent vulnerability to cardiovascular consequences of chronic emotional stress: Review and perspectives for future research

Crestani

2017

Neuroscience & Biobehavioral Reviews

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Emotional stress has been recognized as a modifiable risk factor for cardiovascular diseases. Adolescence has been proposed as a developmental period of vulnerability to stress. This idea has been mainly supported by experimental research in animals demonstrating a higher impact of chronic emotional stress in adolescents compared with adults. Adolescent vulnerability is also based on evidence that stress during this developmental period affects development, so that enduring changes are found in adult animals that experienced stress during adolescence. The purpose of the present review is to discuss experimental research in rodent models that investigated the impact of long-term exposure to stressful events during adolescence on cardiovascular function. The development of cardiovascular function and autonomic activity in rodents is initially reviewed. Then, a discussion of an adolescent vulnerability to cardiovascular effects of chronic stress is presented. From the reviewed literature, perspective for future research is proposed to better elucidate adolescent vulnerability to cardiovascular complications evoked by chronic emotional stress.

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“…Recently, some progress has been made using comparative proteomic studies to investigate ovarian cancer [2,[6][7][8][9][10][11][12][13]. Several studies have reported hippocampus proteome changes after chronic psychological stress [14][15][16]. However, little is known about the effects of chronic psychological stress on the proteome of ovarian cancer tissue.…”

mentioning

confidence: 99%

Proteomic analysis of human epithelial ovarian cancer xenografts in immunodeficient mice exposed to chronic psychological stress

Gao

Zhang

et al. 2011

Sci. China Life Sci.

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This work aims at comparing alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice as well as exploring the molecular mechanisms linking chronic psychological stress to ovarian cancer oncogenesis and progression. SK-OV-3 cells were injected subcutaneously into nude mice. The stress group was subjected to a chronic physical restraint protocol for 6 h on 35 consecutive days, while the control group was unrestrained. All mice were sacrificed on day 36 after SK-OV-3 cell injection, and tumors were excised. Tumor tissues were processed for 2D gel electrophoresis, mass spectrometry (nanoUPLC-ESI-MS/MS) and Western blotting. The expression of 20 proteins was found to be significantly altered between the stress and control groups, of which 14 were up-regulated, five were down-regulated, and one protein was found only in the stress group. All proteins were identified by UPLC-ESI-MS/MS, and Western blotting results were consistent with those of proteomic methods. The present results provide new evidence relating to the molecular mechanism underlying the relationship between psychological stress and tumor progression. proteomic, psychological stress, ovarian cancer, 2D electrophoresis, UPLC-ESI-MS/MS Citation:Gao J, Gao G L, Zhang Y Y, et al. Proteomic analysis of human epithelial ovarian cancer xenografts in immunodeficient mice exposed to chronic psychological stress.

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Large Scale Hippocampal Cellular Distress may Explain the Behavioral Consequences of Repetitive Traumatic Experiences-A Proteomic Approach

Cited by 8 publications

References 66 publications

Effects of an Early Experience of Reward through Maternal Contact or its Denial on Laterality of Protein Expression in the Developing Rat Hippocampus

Effects of an Early Experience of Reward through Maternal Contact or its Denial on Laterality of Protein Expression in the Developing Rat Hippocampus

Adolescent vulnerability to cardiovascular consequences of chronic emotional stress: Review and perspectives for future research

Proteomic analysis of human epithelial ovarian cancer xenografts in immunodeficient mice exposed to chronic psychological stress

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