Large-scale identification of differentially expressed genes during neurogenesis

Pazman, Cecilia; Castelli, JoAnn C.; Wen, Xian‐Huan; Somogyi, Roland

doi:10.1097/00001756-200003200-00013

Cited by 5 publications

(8 citation statements)

References 8 publications

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“…The comparison of the results of our approach with those of proteomics screening of Maurer et al [14] in NSCs from the adult rat hippocampus shows only a few [4][5][6][7][8] .…”

Section: Differential Expression Of Proteins During Neural Differentimentioning

confidence: 84%

“…Using a number of variations in culture conditions and/or genetic modifi cations, cultured neuronal stem cells can differentiate into different neuronal progeny. The complex events leading to the generation of different phenotypes via distinct developmental programs can be studied systematically with transgenic technology and microarray techniques [4][5][6]8] . However, ultimately the regulatory functions are carried out by the cellular protein constituents, and proteomic approaches are most appropriate to determine protein content, stability and subcellular localization, posttranslational modifications and protein-protein interactions.…”

Section: Proteomic Investigations Of Neuronal (Stem) Cell Linesmentioning

confidence: 99%

“…The differentiation as well as maturation of stem cells involve signifi cantly complex events leading to the generation of different phenotypes via distinctive developmental programs. In contrast to transgenic technologies and microarrays [4][5][6][7][8] , with proteomic approaches important issues such as protein amount, protein stability, subcellular localization of proteins, posttranslational modifi cations and protein-protein interactions can be elucidated. Nowadays, the combination of high-resolution two-dimensional gel electrophoresis and identifi cation of proteins by mass spectrometry (MS) is broadly applied to proteomic profi ling.…”

Section: Introductionmentioning

confidence: 99%

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2-DE Proteomic Profiling of Neuronal Stem Cells

Hoffrogge

Beyer²,

Völker³

et al. 2006

Neurodegener Dis

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Proteomics has become a powerful tool in neuroscience studies. Although numerous human neural stem cells are available for research purposes since many years, there exists only limited information on proteomic data from stable neural stem cell lines. Profiling and functional proteome studies of neuronal stem cells will help to describe the protein inventory as well as protein activity and interactions, subcellular localization and posttranslational modifications. The proteomic analysis of neuronal differentiation processes will elucidate the complex events leading to the generation of different phenotypes via distinctive developmental programs that control self-renewal, differentiation, and plasticity. Using the ReNcell VM197 model, a cell line derived from human fetal ventral mesencephalon stem cells, we studied the protein inventory of the stem cells by 2-DE gel electrophoresis and mass spectrometric protein identification and constructed a 2-DE protein map consisting of more than 400 identified protein spots. This proteome reference database constitutes the basis for further investigations of differential protein expression during differentiation. A profiling of the neuronal differentiation-associated changes displayed the large rearrangement of the proteome during this process, and the proteomic techniques proved to be a valuable tool for the elucidation of neuronal differentiation process and for target protein screening.

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“…The comparison of the results of our approach with those of proteomics screening of Maurer et al [14] in NSCs from the adult rat hippocampus shows only a few [4][5][6][7][8] .…”

Section: Differential Expression Of Proteins During Neural Differentimentioning

confidence: 84%

Section: Proteomic Investigations Of Neuronal (Stem) Cell Linesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

2-DE Proteomic Profiling of Neuronal Stem Cells

Hoffrogge

Beyer²,

Völker³

et al. 2006

Neurodegener Dis

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“…Differential display (DD) has been widely used to detect and isolate genes that respond to growth factors, developmentally regulated genes and genes whose expression correlates with certain diseases (Pazman et al. 2000; Fu, 2002).…”

Section: Methods For Studying Differential Gene Expressionmentioning

confidence: 99%

Strategies for identifying genes that play a role in spinal cord regeneration

et al. 2003

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A search for genes that promote or block CNS regeneration requires numerous approaches; for example, tests can be made on individual candidate molecules. Here, however, we describe methods for comprehensive identification of genes up-and down-regulated in neurons that can and cannot regenerate after injury. One problem concerns identification of low-abundance genes out of the 30 000 or so genes expressed by neurons. Another difficulty is knowing whether a single gene or multiple genes are necessary. When microchips and subtractive differential display are used to identify genes turned on or off, the numbers are still too great to test which molecules are actually important for regeneration. Candidates are genes coding for trophic, inhibitory, receptor and extracellular matrix molecules, as well as unknown genes. A preparation useful for narrowing the search is the neonatal opossum. The spinal cord and optic nerve can regenerate after injury at 9 days but cannot at 12 days after birth. This narrow window allows genes responsible for the turning off of regeneration to be identified. As a next step, sites at which they are expressed (forebrain, midbrain, spinal cord, neurons or glia, intracellular or extracellular) must be determined. An essential step is to characterize proteins, their levels of expression, and their importance for regeneration. Comprehensive searches for molecular mechanisms represent a lengthy series of experiments that could help in devising strategies for repairing injured spinal cord.

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“…Differentiation and maturation of stem cells comprises complex serial events, which guide the undifferentiated cells to different lineages via distinctive developmental programs (Chung et al, 2005;Hoffrogge et al, 2006;Kwak et al, 2006). Such regulatory changes can be studied systematically with transgenic technology and microarrays (Pazman et al, 2000;Böttcher et al, 2003;Gurok et al, 2004;Pahnke et al, 2004). The process of differentiation entails changes in types and amount of proteins expressed by the stem cell.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry based proteomic analysis of human stem cells: a brief review

Choi

An²,

Kim³

et al. 2007

Exp Mol Med

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Stem cells can give rise to various cell types and are capable of regenerating themselves over multiple cell divisions. Pluripotency and self-renewal potential of stem cells have drawn vast interest from different disciplines, with studies on the molecular properties of stem cells being one example. Current investigations on the molecular basis of stem cells pluripotency and self-renewal entail traditional techniques from chemistry and molecular biology. In this mini review, we discuss progress in stem cell research that employs proteomics approaches. Specifically, we focus on studies on human stem cells from proteomics perspective. To our best knowledge, only the following types of human stem cells have been examined via proteomics analysis: human neuronal stem cells, human mesenchymal stem cells, and human embryonic stem cells. Protein expression serves as biomarkers of stem cells and identification and expression level of such biomarkers are usually determined using two-dimensional electrophoresis coupled mass spectrometry or non-gel based mass spectrometry.

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Large-scale identification of differentially expressed genes during neurogenesis

Cited by 5 publications

References 8 publications

2-DE Proteomic Profiling of Neuronal Stem Cells

2-DE Proteomic Profiling of Neuronal Stem Cells

Strategies for identifying genes that play a role in spinal cord regeneration

Mass spectrometry based proteomic analysis of human stem cells: a brief review

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