Large-scale multi-omics analysis suggests specific roles for intragenic cohesin in transcriptional regulation

Abstract

Cohesin, an essential protein complex for chromosome segregation, regulates transcription through a variety of mechanisms. It is not a trivial task to genome-widely assign the diverse cohesin functions. Moreover, the context-specific roles of cohesin-mediated interactions, especially on intragenic regions, have not been thoroughly investigated. Here we performed a comprehensive characterization of cohesin binding sites in several human cell types. We integrated epigenomic, transcriptomic and chromatin interaction data with and without transcriptional stimulation, to explore context-specific functions of intragenic cohesin related to gene activation. We identified a new subset of cohesin binding sites, decreased intragenic cohesin sites (DICs), which have a different function from previously known ones. The intron-enriched DICs were negatively correlated with transcriptional regulation: a subgroup of DICs were related to enhancer markers and paused RNA polymerase II, whereas others contributed to chromatin architecture. We implemented machine learning and successfully isolated DICs with distinct genomic features. We observed DICs in various cell types, including cells from cohesinopathy patients. These results suggest a previously unidentified function of cohesin at intragenic regions for transcription regulation.

Chung–Jansen syndrome can mimic Cornelia de Lange syndrome: Another player among chromatinopathies?

“…(i) molecular findings demonstrate the increasing and substantial role of the cohesin complex in transcriptional regulation (Dorsett, 2011;Maya-Miles et al, 2019;Schwarzer et al, 2017;Wang et al, 2021);…”