2016
DOI: 10.1007/s11816-016-0397-4
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Large-scale production of foreign proteins via the novel plant transient expression system in Pisum sativum L.

Abstract: Transient expression of foreign genes byAgrobacterium infiltration is a versatile technique that can be used as a rapid tool for functional protein production in plants. A reproducible protocol of large-scale production of foreign proteins via the novel plant transient expression system in Pisum sativum L. was established in our study. Non-detached plants from soil-independent culture were used as the target organ, and vacuum infiltrating mediated by Agrobacterium tumefaciens harboring green fluorescent protei… Show more

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