Large-Scale Production of Rosmarinic Acid from Plant Cell Cultures of Coleus blumei Benth.

Ulbrich, Bernhard; Wiesner, W.; Arens, Hans

doi:10.1007/978-3-642-70717-9_28

Cited by 114 publications

(41 citation statements)

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“…Highest fresh biomass and RA content obtained from S. khuzistanica suspension culture were 353.0 g/L and 180.0 mg/g DW after 21 days, respectively. So far, the highest content of RA in suspension cultures was reported in Salvia officinalis (360.0 mg/g DW) by Hippolyte et al (1992) and Coleus blumei (210.0 mg/g DW) by Ulbrich et al (1985). Literature survey revealed that the growth rate of Ocimum basilicum L. and Lavandula vera cell cultures was higher than that of S. khuzistanica, and in contrast with our results, they reached the highest biomass production on days 14 and 8, respectively (Kintzios et al 2003;Georgiev et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

et al. 2015

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An in vitro approach to the production of rosmarinic acid (RA), a medicinally important caffeic acid ester, in a cell suspension culture (CSC) of Satureja khuzistanica Jamzad (Lamiaceae) has been investigated for the first time. The CSC was established from friable calli derived from shoot tip explants in Gamborg's B5 liquid medium supplemented with 30 g/L sucrose, 20 mg/L L-glutamine, 200 mg/L casein hydrolysate, 5 mg/L benzyladenine (BA) and 1 mg/L indole-3-butyric acid (IBA). The effect of nitrogen source (KNO 3 and (NH 4 ) 2 SO 4 ) and their different concentrations on the fresh and dry weight (g/L), as well as RA content (mg/g dry weight)were measured. CSC growth measurements indicated a maximum specific cell growth rate of 1.5/day, a doubling time of 7.6 days and a high percentage of cell viability (96.4 %) throughout the growth cycle. Maximum cell fresh weight (353.5 g/L), dry weight (19.7 g/L) and RA production (180.0 mg/g) were attained at day 21 of culture. Cell growth and RA content were affected by nitrogen deficiency. Media containing 8.3 mM of total nitrogen ( of B5 standard medium) led to a minimum cell fresh weight (243.0 g/ L), dry weight (17.4 g/L) and RA content (38.0 mg/g) after 21 days. The established CSC provided useful material for further optimization experiments aimed at a large-scale production of RA.

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Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

et al. 2015

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“…Thus, the RA content in hairy roots in bioreactor was two times higher compared to that in fl asks/Petri dishes. Rosmarinic acid production in laboratory bioreactors was investigated in cell cultures of C. blumei (42,43) and other species (44)(45)(46) while, to our knowledge, this is the fi rst report where a hairy root culture in a bioreactor has been ex- HPLC chromatograms (f) of the extracts from normal roots a er acid hydrolysis (NR NAA) compared to the hydrolysed extracts from hairy roots (HR 0) and hairy roots cultured on MS with 1 mg/L of NAA (HR NAA). The chromatograms were obtained using a gradient elution system (see Materials and Methods) and detection was carried out at 280 nm.…”

Section: The Eff Ect Of the Cultivation Methods On Growth And Rosmarinmentioning

confidence: 99%

Potential of Different Coleus blumei Tissues for Rosmarinic Acid Production

Bauer

Vuković

Likić

et al. 2015

Food Technol. Biotechnol.

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SummaryRosmarinic acid is one of the main active components of Coleus blumei and is known to have numerous health benefi ts. The pharmacological signifi cance of rosmarinic acid and its production through in vitro culture has been the subject of numerous studies. Here, the ability of diff erent tissues to accumulate rosmarinic acid and sustainability in production over long cultivation have been tested. Calli, tumours, normal roots and hairy roots were established routinely by application of plant growth regulators or by transformation with agrobacteria. The diff erences among the established tumour lines were highly heterogeneous. Hairy root lines showed the highest mean growth rate and consistency in rosmarinic acid production. Although some tumour lines produced more rosmarinic acid than the hairy root lines, over a long cultivation period their productivity was unstable and decreased. Further, the eff ects of plant growth regulators on growth and rosmarinic acid accumulation were tested. 2,4-Dichlorophenoxyacetic acid signifi cantly reduced tumour growth and rosmarinic acid production. 1-Naphthaleneacetic acid strongly stimulated hairy root growth whilst abscisic acid strongly enhanced rosmarinic acid production. Hairy roots cultured in an airli bioreactor exhibited the highest potential for mass production of rosmarinic acid.

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“…Thus in some cases expression of a pathway is evidently better Yamada (1986) All productivities were calculated from small-size experiments never exceeding culture volumes of 100m!. Zenk et al (1975), Bauch and Leistner (1978), Schulte et al (1984) Fujita et al (1981) Nagakawa et al (1984, Sato and Yamada (1984), Hinz and Zenk (1981), Breuling et al (1985) Most of these high-producing cell cultures have been grown in volumes of 10-800 I and their productivities were even better under the controlled conditions of fermentation than in shake flasks (see Schiel et al 1984a, b;Ulbrich et al 1985;Breuling et al 1985).…”

Section: De-novo Synthesis Of Known Drugs By Cell Culturesmentioning

confidence: 99%

“…Rosmarinic acid is found spontaneously at high levels in cultures of Anchusa, Coleus, Rosmarinus or Salvia. Ulbrich et al (1985) reported a production rate of 0.9 g rosmarinic acid/lid. As an antiphlogistic activity of rosmarinic acid has been demonstrated, this seemingly unimportant compound may become an interesting candidate for production by cell cultures.…”

Section: De-novo Synthesis Of Known Drugs By Cell Culturesmentioning

confidence: 99%

“…The first commercial process, the production of shikonins, is also performed in stirred tanks (Fujita et al 1982). The productivity of culture systems can often be further improved under the better controlled conditions of fermentation (Schiel et al 1984b;Ulbrich et al 1985). Thus, provided a rapidly growing, highly productive cell line is available, the technological problems of scale up seem to be resolvable.…”

Section: Introductionmentioning

confidence: 99%

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Formation of Secondary Metabolites in Cultured Plant Cells and Its Impact on Pharmacy

Berlin

1988

Biotechnology in Agriculture and Forestry

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Large-Scale Production of Rosmarinic Acid from Plant Cell Cultures of Coleus blumei Benth.

Cited by 114 publications

References 11 publications

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

Potential of Different Coleus blumei Tissues for Rosmarinic Acid Production

Formation of Secondary Metabolites in Cultured Plant Cells and Its Impact on Pharmacy

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