HPLC of Peptides and Proteins
DOI: 10.1385/1-59259-742-4:211
|View full text |Cite
|
Sign up to set email alerts
|

Large-Scale Protein Chromatography

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

0
3
0

Publication Types

Select...
3

Relationship

0
3

Authors

Journals

citations
Cited by 3 publications
(3 citation statements)
references
References 2 publications
0
3
0
Order By: Relevance
“…After the optional addition of charged groups ( 5), a fraction of remaining hydroxyl groups was esterified with 2-chloropro- pionoyl chloride to produce the ATRP initiator-functionalized surface (6). When using limiting quantities of 2-chlorpropionoyl chloride, incorporation of ATRP initiators via esterification had low reaction efficiency (13%), perhaps due to small amounts of water remaining on the base material.…”
Section: Aqueous Atrp Graft Polymerization Of Pdma Frommentioning
confidence: 99%
See 1 more Smart Citation
“…After the optional addition of charged groups ( 5), a fraction of remaining hydroxyl groups was esterified with 2-chloropro- pionoyl chloride to produce the ATRP initiator-functionalized surface (6). When using limiting quantities of 2-chlorpropionoyl chloride, incorporation of ATRP initiators via esterification had low reaction efficiency (13%), perhaps due to small amounts of water remaining on the base material.…”
Section: Aqueous Atrp Graft Polymerization Of Pdma Frommentioning
confidence: 99%
“…Therefore, there is considerable interest in new strategies for synthesizing SEC media that permit one to carefully “tune” the properties of the stationary phase to maximum separation efficiency over a desired molecular weight range. This need has intensified in recent years due to the growth of proteomics, which requires highly efficient strategies for separating complex mixtures, and by the shift in bioprocessing of blood products away from the classic cold ethanol precipitation process of Cohn to much more efficient and selective chromatography trains. , Human blood plasma is a source material for many life-saving therapeutics, including albumin, intravenous immune globulin, α-antiplasmin, α 1 -antitrypsin, and a number of coagulation proteins. As it is now thought to contain more than 1500 unique peptides, , human plasma has the potential to yield a number of new protein-based therapeutics.…”
Section: Introductionmentioning
confidence: 99%
“…Since then, it has been used at preparative scales in the manufacture of insulin (Janson, 1971) and in the purification of human serum albumin (HSA) from blood plasma (Lin et al, 2000;Curling, 1980). First reported by Friedli and Kistler (1972), the removal of ethanol from HSA purified by the Cohn-Oncley cold-ethanol fractionation process remains one of the largest and most well-documented applications of SEC (Johnston and Adcock, 2000;Bertolini et al, 2004).…”
Section: Introductionmentioning
confidence: 99%