Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography

Niu, Jianfeng; Wang, Guangce; X, Lin; Zhou, Biao

doi:10.1016/j.jchromb.2006.11.043

Cited by 87 publications

(52 citation statements)

References 28 publications

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“…In Japan, the use of phycocyanin as a food dye has been authorized for products like chewing gums, candies, fermented milk products, ice creams, soft drinks, alcoholic drinks, desserts, cake decorations, milk shakes and dairy products [2], Niu et al [16]. Moreover, it plays an important role in many biochemical techniques due to its fluorescent properties [15].…”

Section: Introductionmentioning

confidence: 99%

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

Silveira

Quines

Burkert

et al. 2008

Bioprocess Biosyst Eng

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This paper presents the evaluation of some important parameters for the purification of phycocyanin using ion exchange chromatography. The influences of pH and temperature on the equilibrium partition coefficient were investigated to establish the best conditions for phycocyanin adsorption. The equilibrium isotherm for the phycocyanin-resin system was also determined. The separation of phycocyanin using the Q-Sepharose ion exchange resin was evaluated in terms of the pH and elution volume that improved the increase in purity and recovery. The highest partition coefficients were obtained in the pH range from 7.5 to 8.0 at 25 degrees C. Under these conditions the equilibrium isotherm for phycocyanin adsorption was well described by the Langmuir model, attaining a Q (m) of 22.7 mg/mL and K (d) of 3.1 x 10(-2) mg/mL. The best conditions for phycocyanin purification using the ion exchange column were at pH 7.5 with an elution volume of 36 mL, obtaining 77.3% recovery and a 3.4-fold increase in purity.

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Section: Introductionmentioning

confidence: 99%

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

Silveira

Quines

Burkert

et al. 2008

Bioprocess Biosyst Eng

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“…Patil et al [77] S. platensis Chitosan adsorption; Two-phase aqueous extraction 5.1 66.0 PC Patil et al [77] S. platensis Chitosan adsorption; Two-phase aqueous extraction; Ion exchange chromatography 6.69 -PC Niu et al [78] S. platensis Expanded bed adsorption chromatography; Ion exchange chromatography 3.64 8.7…”

Section: Pcmentioning

confidence: 99%

Recent advances in production, purification and applications of phycobiliproteins

Sonani

Rastogi

Patel

et al. 2016

WJBC

159

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light-harvesting, PBPs are found to involve in several life sustaining phenomena including photoprotection in cyanobacteria. The unique spectral features (like strong absorbance and fluorescence), proteineous nature and, some imperative properties like hepato-protective, antioxidants, anti-inflammatory and anti-aging activity of PBPs enable their use in food, cosmetics, pharmaceutical and biomedical industries. PBPs have been also noted to show beneficial effect in therapeutics of some disease like Alzheimer and cancer. Such large range of applications increases the demand of PBPs in commodity market. Therefore, the large-scale and coast effective production of PBPs is the real need of time. To fulfil this need, many researchers have been working to find the potential producer of PBPs for the production and purification of PBPs. Results of these efforts have caused the inventions of some novel techniques like mixotrophic and heterotrophic strategies for production and aqueous two phase separation for purification purpose. Overall, the present review summarises the recent findings and identifies gaps in the field of production, purification and applications of this biological and economically important proteins. Core tip: The present review compiles the overall research that has been done so far in the field of produ ction, purification and application of phycobiliptoteins (PBPs). The comparative accounts for different methods for PBPs production, purification have been discussed. The potential applications of PBPs in various industries including food, cosmetics and pharmaceutical have been debated rationally. Remaining questions and rese arch gaps in respected field have been highlighted as conclusion. Recent advances in production, purification and applications of phycobiliproteins AbstractAn obligatory sunlight requirement for photosynthesis has exposed cyanobacteria to different quantity and quality of light. Cyanobacteria can exhibit efficient photosynthesis over broad region (450 to 650 nm) of solar spectrum with the help of brilliantly coloured pigment proteins called phycobiliproteins (PBPs). Besides REVIEW 100February 26, 2016|Volume 7|Issue 1|

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“…Niu et al [20] reported that with the use of an expanded bed, many of the concentration and partial isolation steps and procedures, such as precipitation in ammonium sulfate and gel-filtration used in conventional chromatographic purification protocols, are eliminated. A typical cycle of the isolation process by EBA chromatography including equilibration (30 min) ?…”

Section: Runmentioning

confidence: 99%

“…Niu et al [20] used hydrophobic interaction in an expanded bed to purify a crude extract of C-phycocyanin without cells, and obtained a C-phycocyanin extract that was four times purer with a recovery of 35% and yield of 31 mg g -1 . In the present paper, if the fraction collected were just more purified, a product with an increment of four times in purity and a recovery of about 50% would be obtained.…”

Section: Runmentioning

confidence: 99%

Recovery of C-Phycocyanin in the Presence of Cells Using Expanded Bed IEC

et al. 2011

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C-Phycocyanin is a natural blue dye that can be extracted from Spirulina platensis. In this paper, a crude extract of C-phycocyanin in the presence of cells was purified using ion exchange chromatography in an expanded bed. The elution was studied aiming at its optimization using a central composite rotatable design (CCRD), the independent variables being the pH and elution volume, and the responses purity, concentration, yield and recovery. An evaluation of the contour curves showed that the best conditions for purification were a pH value of 6.5 and elution volume of about 150 mL. A run was carried out under these conditions, combining a saline gradient (0-1 M) with step elution using 0.1 M NaCl. Under these conditions, it was possible to obtain C-phycocyanin with a purity increment of 4.6-fold compared with the initial extract working directly with unclarified extract.

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Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography

Cited by 87 publications

References 28 publications

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

Recent advances in production, purification and applications of phycobiliproteins

Recovery of C-Phycocyanin in the Presence of Cells Using Expanded Bed IEC

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