Large-scale screening of rhizobacteria to enhance the chickpea-Mesorhizobium symbiosis using a plant-based strategy

Alemneh, Anteneh Argaw; Zhou, Yi; Ryder, Maarten H.; Denton, Matthew D.

doi:10.1016/j.rhisph.2021.100361

Cited by 14 publications

(10 citation statements)

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“…Therefore, the positive effect of this strain might be related to its ability to solubilize inorganic phosphate up to 690.86 μg ml −1 and its ammonia production ( Table 2 ). Similar results were reported in Alemneh et al (2021) , who observed that several strains belonging to Bacillus spp. enhanced the growth and nodulation of chickpeas under gnotobiotic conditions.…”

Section: Discussionsupporting

confidence: 90%

“…Plant-related factors in the rhizosphere, such as the production of organic compounds in root exudates, might affect the survival and colonization of PGPR and their ability to express many PGP activities ( Drogue et al, 2012 ; Alemneh et al, 2021 ). Therefore, we used a plant-based strategy for screening PGPR regarding their PGP potential.…”

Section: Discussionmentioning

confidence: 99%

“…The cross-talk between IAA and ACCD is fundamental for PGPR to enhance root growth ( Glick, 2014 ). Several studies have reported that PGPR producing IAA higher than 40 μg ml −1 inhibited root growth and seed germination ( Pawlik et al, 2017 ; Alemneh et al, 2021 ) due to the stimulation of ethylene caused by the higher amount of IAA ( Glick, 2014 ). However, if the bacterium has both IAA and ACC deaminase activities, then the ACCD would mediate the decreasing of ethylene production, thus permitting IAA synthesis, which could continue to enhance root growth ( Glick, 2014 ; Kang et al, 2019 ; Alemneh et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported that PGPR producing IAA higher than 40 μg ml −1 inhibited root growth and seed germination ( Pawlik et al, 2017 ; Alemneh et al, 2021 ) due to the stimulation of ethylene caused by the higher amount of IAA ( Glick, 2014 ). However, if the bacterium has both IAA and ACC deaminase activities, then the ACCD would mediate the decreasing of ethylene production, thus permitting IAA synthesis, which could continue to enhance root growth ( Glick, 2014 ; Kang et al, 2019 ; Alemneh et al, 2021 ). Several other PGPR in our study that promoted root growth and produced both IAA and ACC deaminase included strains such as Microbacterium oxydans strain SB39, Pseudomonas mosselii strain SB45, Pseudomonas stutzeri strain SB38, P. plecoglossicida strain ET27, S. pavanii strain EB31, G. amicalis strain WT12, and Klebsiella oxytoca strain EA5 ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

“…enhanced the growth and nodulation of chickpeas under gnotobiotic conditions. The improvement of plant growth was mainly related to the ability of these strains to express PGP traits other than IAA and ACCD ( Alemneh et al, 2021 ). Interestingly, bacterial strains tested in this experiment showed growth-promoting potential for canola plants despite being isolated from different plant species.…”

Section: Discussionmentioning

confidence: 99%

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In-Depth Characterization of Plant Growth Promotion Potentials of Selected Alkanes-Degrading Plant Growth-Promoting Bacterial Isolates

2022

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The use of plant growth-promoting rhizobacteria (PGPR) as a bioremediation enhancer in plant-assisted phytoremediation requires several steps, consisting of the screening, selection, and characterization of isolates. A subset of 50 bacterial isolates representing a wide phylogenetic range were selected from 438 morphologically different bacteria that were originally isolated from a petroleum hydrocarbon (PHC)-polluted site of a former petrochemical plant. Selected candidate bacteria were screened using six conventional plant growth-promoting (PGP) traits, complemented with the genetic characterization of genes involved in alkane degradation, as well as other pertinent functions. Finally, the bacterial isolates were subjected to plant growth promotion tests using a gnotobiotic approach under normal and stressed conditions. Our results indicated that 35 bacterial isolates (70%) possessed at least four PGP traits. Twenty-nine isolates (58%) were able to utilize n-hexadecane as a sole carbon source, whereas 43 isolates (86%) were able to utilize diesel as the sole carbon source. The presence of catabolic genes related to hydrocarbon degradation was assessed using endpoint PCR, with the alkane monooxygenase (alkB) gene found in 34 isolates, the cytochrome P450 hydroxylase (CYP153) gene found in 24 isolates, and the naphthalene dioxygenase (nah1) gene found to be present in 33 isolates. Thirty-six strains (72%) promoted canola root elongation in the growth pouch assay. After several rounds of screening, seven bacterial candidates (individually or combined in a consortium) were tested for canola root and shoot growth promotion in substrates amended by different concentrations of n-hexadecane (0%, 1%, 2%, and 3%) under gnotobiotic conditions. Our results showed that Nocardia sp. (WB46), Pseudomonas plecoglossicida (ET27), Stenotrophomonas pavanii (EB31), and Gordonia amicalis (WT12) significantly increased the root length of canola grown in 3% n-hexadecane compared with the control treatment, whereas Nocardia sp. (WB46) and Bacillus megaterium (WT10) significantly increased shoot length compared to control treatment at the same concentration of n-hexadecane. The consortium had a significant enhancement effect on root length compared to all isolates inoculated individually or to the control. This study demonstrates that the combination of PGPR traits and the PHC degradation potential of bacteria can result in an enhanced beneficial effect in phytoremediation management, which could lead to the development of innovative bacterial inoculants for plants to remediate PHC-contaminated soils.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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In-Depth Characterization of Plant Growth Promotion Potentials of Selected Alkanes-Degrading Plant Growth-Promoting Bacterial Isolates

2022

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Combination of silicon and plant growth promoting rhizobacteria consortia promoted the growth of melon seedlings under salt stress

Guo,

Liu

et al. 2024

Hortic. Environ. Biotechnol.

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Evaluating the effectiveness of rhizobacteria producing 1-aminocyclopropane-1-carboxylic acid deaminase in inhibiting tumor formation by Agrobacterium tumefaciens

Amer,

Mikhail,

Youssef

et al. 2024

J Plant Dis Prot

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Crown gall is one of the most dangerous bacterial diseases affecting the production of fruit tree nurseries in Egypt and many countries of the world. In the present study, ten isolates of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing rhizobacteria were isolated from the rhizosphere of apricot (Prunus armeniaca L.) and plum (Prunus domestica L.) trees to evaluate their ability to decrease tumor formation by Agrobacterium tumefaciens (synonym Rhizobium radiobacter). The ten isolates were identified as Pseudomonas strains based on 16S rRNA gene sequence analysis and deduced protein sequences obtained from a partial ACC deaminase structural gene (acdS) sequence. Co-inoculating castor bean (Ricinus communis L.) and kalanchoe (Kalanchoe sp.) plants with A. tumefaciens and four ACC deaminase-producing Pseudomonas isolates decreased tumor formation. However, six ACC deaminase-producing Pseudomonas isolates produced varying results in these two plant species. The results showed that isolates of Pseudomonas vancouverensis reduced tumor formation when co-inoculated with A. tumefaciens in castor bean and kalanchoe plants. However, the isolate P. putida inhibited tumor formation in castor bean plants but did not achieve the same effect in kalanchoe plants. Additionally, isolates of P. frederiksbergensis and P. kilonensis decreased tumor formation in kalanchoe plants while increasing tumor formation in castor bean plants. The results showed that ACC deaminase-producing P. vancouverensis is a promising biocontrol agent against A. tumefaciens.

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Large-scale screening of rhizobacteria to enhance the chickpea-Mesorhizobium symbiosis using a plant-based strategy

Cited by 14 publications

References 57 publications

In-Depth Characterization of Plant Growth Promotion Potentials of Selected Alkanes-Degrading Plant Growth-Promoting Bacterial Isolates

In-Depth Characterization of Plant Growth Promotion Potentials of Selected Alkanes-Degrading Plant Growth-Promoting Bacterial Isolates

Combination of silicon and plant growth promoting rhizobacteria consortia promoted the growth of melon seedlings under salt stress

Evaluating the effectiveness of rhizobacteria producing 1-aminocyclopropane-1-carboxylic acid deaminase in inhibiting tumor formation by Agrobacterium tumefaciens

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