Large-scale study of blood markers in equine atypical myopathy reveals subclinical poisoning and advances in diagnostic and prognostic criteria

Renaud, Benoît; Kruse, Caroline-J.; François, Anne-Christine; Cesarini, Carla; van Loon, Gunther; Palmers, Katrien; Boemer, François; Luis, Géraldine; Gustin, Pascal; Votion, Dominique-Marie

doi:10.1016/j.etap.2024.104515

Environmental Toxicology and Pharmacology

2024

DOI: 10.1016/j.etap.2024.104515

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Large-scale study of blood markers in equine atypical myopathy reveals subclinical poisoning and advances in diagnostic and prognostic criteria

Benoît Renaud,

Caroline-J. Kruse,

Anne-Christine François

et al.

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2024

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Cited by 2 publications

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An ongoing concern: 20 years of research on equine atypical myopathy

François,

Renaud,

Kruse

et al. 2024

Veterinary Record

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An ongoing concern: 20 years of research on equine atypical myopathy

François,

Renaud,

Kruse

et al. 2024

Veterinary Record

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A Zebrafish Embryo Model to Screen Potential Therapeutic Compounds in Sapindaceae Poisoning

Wouters,

Klein,

Price

et al. 2024

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Hypoglycin A (HGA) and methylenecyclopropylglycine (MCPrG) are protoxins produced by Sapindaceae plants, particularly Acer pseudoplatanus, and are responsible for causing atypical myopathy (AM) in equids. These protoxins metabolise into toxic compounds, such as methylenecyclopropylacetyl-CoA (MCPA-CoA), which alters energy metabolism and induces severe rhabdomyolysis. Currently, no specific treatment exists for this poisoning, in vitro models fail to reproduce HGA’s toxic effects on equine primary myoblasts, and mammalian models are impractical for large-scale drug screening. This study aimed to develop a zebrafish embryo model for screening therapeutic compounds against AM. Zebrafish embryos were exposed to various concentrations of HGA, MCPrG, and methylenecyclopropylacetate (MCPA) for 72 h. MCPrG did not induce toxicity, while HGA and MCPA showed median lethal concentration (LC50) values of 1.7 µM and 1 µM after 72 h, respectively. The highest levels of the conjugated metabolite MCPA–carnitine were detected 24 h after HGA exposure, and the acylcarnitines profile was highly increased 48 h post-exposure. Isovaleryl-/2- methylbutyrylcarnitine levels notably rose after 24 h, suggesting potential exposition biomarkers. Glycine and carnitine effectively reduced mortality, whereas riboflavin showed no protective effect. These findings suggest that the zebrafish embryo represents a valuable model for identifying therapeutic compounds for Sapindaceae poisoning.

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Large-scale study of blood markers in equine atypical myopathy reveals subclinical poisoning and advances in diagnostic and prognostic criteria

Cited by 2 publications

References 35 publications

An ongoing concern: 20 years of research on equine atypical myopathy

An ongoing concern: 20 years of research on equine atypical myopathy

A Zebrafish Embryo Model to Screen Potential Therapeutic Compounds in Sapindaceae Poisoning

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