Large scale survey of enteric viruses in river and waste water underlines the health status of the local population

Prévost, Benoit; Lucas, Françoise; Gonçalves, Alexandre Leopoldo; Richard, Fanny; Moulin, Laurent; Wurtzer, Sébastien

doi:10.1016/j.envint.2015.03.004

Cited by 170 publications

(108 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For each effluent sample (n ϭ 100), one-step reverse transcription-quantitative PCR (RT-qPCR) assays for HAstV, NoV GI, and NoV II were performed in a reaction volume of 20 l containing 5 l of RNA, primers and probes specific for each virus, and TaqMan fast virus 1-step master mix (Life Technologies, Carlsbad, CA) according to the manufacturer's recommendations. These assays, primers, and probes were described previously (12). Each amplification run included a no-template control and a positive amplification control based on the plasmid used to prepare the standard curves.…”

Section: Methodsmentioning

confidence: 99%

“…A large quantity of viral particles is excreted in the feces of infected people (6,7), and they are finally circulated through the wastewater network (8,9). Viruses are generally not eliminated by wastewater treatment plants (WWTPs), and as a consequence, they can be released into rivers at noticeable levels (10)(11)(12). Consequently, we assume that the viruses in WWTP effluents could represent the viruses circulating not only in environmental waters but also in the human population.…”

mentioning

confidence: 99%

“…Recent studies suggested the existence of a relationship between the viruses spread in a sewerage network and the health status of the local population (12). In order to estimate this relationship, seasonal monitoring of the concentration and diversity of HAstV, NoV GI, and NoV GII strains in WWTP effluents from the Paris, France, urban area was performed over 1 year.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

Prévost

Lucas

Ambert-Balay³

et al. 2015

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

cAlthough clinical epidemiology lists human enteric viruses to be among the primary causes of acute gastroenteritis in the human population, their circulation in the environment remains poorly investigated. These viruses are excreted by the human population into sewers and may be released into rivers through the effluents of wastewater treatment plants (WWTPs). In order to evaluate the viral diversity and loads in WWTP effluents of the Paris, France, urban area, which includes about 9 million inhabitants (approximately 15% of the French population), the seasonal occurrence of astroviruses and noroviruses in 100 WWTP effluent samples was investigated over 1 year. The coupling of these measurements with a high-throughput sequencing approach allowed the specific estimation of the diversity of human astroviruses (human astrovirus genotype 1 [HAstV-1], HAstV-2, HAstV-5, and HAstV-6), 7 genotypes of noroviruses (NoVs) of genogroup I (NoV GI.1 to NoV GI.6 and NoV GI.8), and 16 genotypes of NoVs of genogroup II (NoV GII.1 to NoV GII.7, NoV GII.9, NoV GII.12 to NoV GII.17, NoV GII.20, and NoV GII.21) in effluent samples. Comparison of the viral diversity in WWTP effluents to the viral diversity found by analysis of clinical data obtained throughout France underlined the consistency between the identified genotypes. However, some genotypes were locally present in effluents and were not found in the analysis of the clinical data. These findings could highlight an underestimation of the diversity of enteric viruses circulating in the human population. Consequently, analysis of WWTP effluents could allow the exploration of viral diversity not only in environmental waters but also in a human population linked to a sewerage network in order to better comprehend viral epidemiology and to forecast seasonal outbreaks.T he epidemiology of viral gastroenteritis is mainly evaluated through the use of clinical data. However, clinical data provide only a partial vision of the gastroenteritis viruses circulating in the human population. Indeed, many viral infections do not require specialized consultations or hospitalizations and so are not directly included in clinical statistics. As a consequence, these data sets offer a limited view of the diversity and occurrence of enteric viruses in the human population.Human astroviruses (HAstVs) and noroviruses (NoVs) are among the main causes of human acute gastroenteritis worldwide (1, 2). HAstVs and NoVs are nonenveloped viruses and have a positive-sense single-stranded RNA. The genome of HAstV is composed of three open reading frames (ORFs), and ORF2, encoding the capsid protein precursor, allows discrimination of eight genotypes of HAstV, HAstV-1 to HAstV-8 (3). The genome of NoV is also composed of three ORFs, and genotyping based on the genetic similarity of ORF2 and ORF3 allows discrimination of 9 genotypes of NoV genogroup I (NoV GI) and 22 genotypes of NoV genogroup II (NoV GII), which are the most common genogroups associated with human infections.HAstVs and NoVs are able to infect i...

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

Prévost

Lucas

Ambert-Balay³

et al. 2015

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Além disso, a disseminação de vírus entéricos em ambientes aquáticos localizados no espaço urbano é preocupante, considerando a persistência ambiental desses agentes e a resistência que apresentam a processos convencionais de tratamento de água e esgoto 8,9 . Os vírus entéricos atingem as águas superficiais especialmente por meio do lançamento de efluentes domésticos e agropecuários, uma vez que, são eliminados em altas concentrações nas fezes de humanos e animais infectados 10,11 . Cabe destacar, que são escassos os estudos na literatura que relatam a presença de adenovírus e rotavírus em águas superficiais e subterrâneas bem como em esgoto bruto e tratado.…”

Section: Métodologia Introduçãounclassified

Adenovírus e rotavírus em águas superficiais do córrego Ribeirão Preto, São Paulo, Brasil

et al. 2016

View full text Add to dashboard Cite

ResumoCom o desenvolvimento humano, os recursos hídricos sofrem modificações derivadas da expansão demográfica e principalmente da falta de saneamento básico, que interferem diretamente na qualidade desses recursos com a introdução de substâncias químicas tóxicas e bioagentes patogênicos. Os vírus entéricos de veiculação hídrica, como adenovírus e rotavírus, merecem destaque considerando seus impactos na saúde pública. O objetivo deste estudo foi avaliar a presença de adenovírus e rotavírus em águas superficiais do Córrego Ribeirão Preto, SP. A coleta foi realizada em 11 pontos distintos, da nascente até a confluência com o Rio Pardo. O Teste de ELISA foi o método utilizado para a detecção dos vírus (RIDASCREEN® Adenovirus, RIDASCREEN® Rotavirus -R-Biopharm). De acordo com os resultados obtidos, 73% das amostras foram positivas para adenovírus, enquanto que 36% foram positivas para rotavírus. Considerando a virulência desses vírus entéricos e a vulnerabilidade de indivíduos imunossuprimidos, idosos e crianças, é preocupante a contaminação por esses agentes patogênicos de veiculação hídrica amplamente disseminados em águas superficiais. Palavras-chave: AbstractWater resources have been suffering with the changes in demographic expansion and the lack of basic sanitation. These factors directly interfere with the quality of these resources due to the discharge of toxic chemicals and pathogenic bio-agents. The waterborne enteric viruses, such as rotavirus and adenovirus, deserve attention considering its impact on public health. The aim of this study was to evaluate the presence of adenovirus and rotavirus in surface water of Ribeirão Preto stream. Samplings were conducted in 11 different points from the spring to the confluence with the Rio Pardo. The ELISA test was carried out for the detection of viruses (RIDASCREEN® adenovirus, rotavirus RIDASCREEN® -R-Biopharm). According to the results, 73% of the samples were positive for adenovirus, while 36% were positive for rotavirus. Given the virulence of these enteric viruses and the vulnerability of immunosuppressed individuals, i.e. the elderly and children, contamination with these waterborne pathogens widely disseminated in surface water is concerning.

show abstract

“…In addition, research has shown a lack of correlation in the presence of viral pathogens and faecal indicator bacteria [9,10] whose sole presence or absence cannot be used to accurately predict the presence of other pathogens such as protozoan parasites and enteric viruses which are highly infectious in low doses [11]. Most sewage treatment plants fail to completely remove enteric viruses from wastewater either due to the resistance of viruses to adverse conditions or simply because the treatment plants are generally not designed to remove viral pathogens as they are to reduce the biochemical oxygen demand levels of the wastewater [12].…”

Section: A Rt I C L E I N F O Abstractmentioning

confidence: 99%

Assessment of the prevalence of enteric viruses in the final effluents of two peri-urban wastewater treatment plants

Gcilitshana¹,

Sibanda²,

Zhou³

et al. 2017

Asian Pac J Trop Dis

View full text Add to dashboard Cite

Large scale survey of enteric viruses in river and waste water underlines the health status of the local population

Cited by 170 publications

References 35 publications

Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

Adenovírus e rotavírus em águas superficiais do córrego Ribeirão Preto, São Paulo, Brasil

Assessment of the prevalence of enteric viruses in the final effluents of two peri-urban wastewater treatment plants

Contact Info

Product

Resources

About