Large volume sample stacking of antiepileptic drugs in counter current electrophoresis performed in PAMAPTAC coated capillary

Tůma, Petr; Hložek, Tomáš; Sommerová, Blanka; Koval, Dušan

doi:10.1016/j.talanta.2020.121626

Cited by 17 publications

(14 citation statements)

References 44 publications

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“…In principle, the analyte is not lost during forcing out of the matrix using EOF as, if the analyte is to reach the detector, its electrophoretic speed must be greater than the speed of the EOF acting in the opposite direction and both are controlled by the intensity of the separation field. Contrary, the forcing out of the matrix by an external pressure is accompanied by sample losses, as was demonstrated in our previous work [ 49 ]. With PAMAPTAC coating, the magnitude of the EOF is controlled by the content of cationic APTAC components in the polymerisation mixture, where 6% PAMAPTAC coating was found suitable for the separation of methylarginines [ 50 ].…”

Section: Resultsmentioning

confidence: 77%

“…The capillary parameters are a 25 µm inner diameter (ID), 360 µm outer diameter (OD), total length of 39.45 cm and length to C 4 D 25.05 cm. The PAMAPTAC capillary was prepared in the laboratory according the procedure in [ 53 , 54 ] and described in detail in our recent publications [ 49 , 50 ]. The 6% PAMAPTAC capillary was characterised by an anodic EOF mobility with a value of (−17.3 ± 0.0) 10 −9 m 2 V −1 s −1 in 750 mM AcOH as background electrolyte (BGE).…”

Section: Methodsmentioning

confidence: 99%

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Electrophoretic Determination of Symmetric and Asymmetric Dimethylarginine in Human Blood Plasma with Whole Capillary Sample Injection

Tůma

Sommerová

Koval

et al. 2021

IJMS

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Asymmetric and symmetric dimethylarginines are toxic non-coded amino acids. They are formed by post-translational modifications and play multifunctional roles in some human diseases. Their determination in human blood plasma is performed using capillary electrophoresis with contactless conductivity detection. The separations are performed in a capillary covered with covalently bonded PAMAPTAC polymer, which generates anionic electroosmotic flow and the separation takes place in the counter-current regime. The background electrolyte is a 750 mM aqueous solution of acetic acid with pH 2.45. The plasma samples for analysis are treated by the addition of acetonitrile and injected into the capillary in a large volume, reaching 94.5% of the total volume of the capillary, and subsequently subjected to electrophoretic stacking. The attained LODs are 16 nm for ADMA and 22 nM for SDMA. The electrophoretic resolution of both isomers has a value of 5.3. The developed method is sufficiently sensitive for the determination of plasmatic levels of ADMA and SDMA. The determination does not require derivatization and the individual steps in the electrophoretic stacking are fully automated. The determined plasmatic levels for healthy individuals vary in the range 0.36–0.62 µM for ADMA and 0.32–0.70 µM for SDMA.

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Section: Resultsmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Electrophoretic Determination of Symmetric and Asymmetric Dimethylarginine in Human Blood Plasma with Whole Capillary Sample Injection

Tůma

Sommerová

Koval

et al. 2021

IJMS

Self Cite

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“…To avoid unwanted adsorption, coating of the capillary wall is a common strategy. Capillary coatings include polymer coatings (e.g., poly (diallyl dimethyl ammonium chloride) [63,65], polyvinyl alcohol [66], polybrene) or successive multiple ionic layer-coatings [67]. Capillary coating based on Si-C linkages to N-acryloylamido ethoxyethanol with a new synthetic strategy, LiAlH 4 surface reaction, is explained by Meixner et al [63].…”

Section: Capillary Technologiesmentioning

confidence: 99%

“…Fused-silica capillary covalently coated with the copolymer of acrylamide with 5% content of permanently charged 3-acrylamidopropyl trimethylammonium chloride (PAM-ATAC), was successfully developed for the analysis of antiepileptic drugs. The PAM-ATAC coating reduced sorption of the analytes on the capillary wall, which acted against the direction of the electrophoretic migration of the analytes resulting in baseline separation of vigabatrin, pregabalin, and gabapentin in human serum [65]. Yu et al developed a covalently connected capillary coating using diazotized poly(vinyl alcohol-b-styrene) as a photosensitive coating agent.…”

Section: Capillary Technologiesmentioning

confidence: 99%

Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Suntornsuk

Anurukvorakun

2022

Electrophoresis

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This review aims to illustrate sensitivity enhancement methods in capillary electrophoresis (CE) and their applications for pharmaceutical and related biochemical substance analyses. The first two parts of the article describe the introduction and principle of CE. The main part focuses on strategies for sensitivity improvement in CE including detector and capillary technologies and preconcentration techniques. Applications of these techniques for pharmaceutical and biomedical substance analyses are surveyed during the years 2018-2021.

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“…The concentration of samples and the detection sensitivity could be increased by changing the migration rate of the target analyte. The common online sample preconcentration techniques include sample stacking [22][23][24][25][26][27], sweeping [28][29][30], transient isotachophoresis [31][32][33][34], dynamic pH junction [35][36][37], and so on. In recent years, online sample preconcentration based on the capillary coated with charged polymer is also a promising way to enhance the detection sensitivity of protein.…”

Section: Introductionmentioning

confidence: 99%

Online preconcentration of lysozyme in hen egg white using responsive polymer coating in CE

Wang

et al. 2021

J of Separation Science

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A mixed polymer brushes material based on poly (2‐methyl‐2‐oxazoline)‐ and poly (acrylic acid)‐coated capillary with switchable protein adsorption/desorption properties was applied for online preconcentration of lysozyme in hen egg white during capillary electrophoresis performance. First, lysozyme in simulated egg white was successfully online preconcentrated and the detection signal of lysozyme was amplified. Ovalbumin, ovomucoid, and conalbumin in egg white were verified show negligible interference on the online preconcentration of lysozyme according to the study on electroosmotic flow mobility. Second, a series validation procedure was carried out to evaluate the proposed method performance. There was a good linearity behavior range from 0.1 to 5.0 ng/mL, limit of detection was 20 pg/mL, and limit of quantity was 50 pg/mL, the accuracy and robustness of this method were also excellent. Last, the proposed method has been successfully used to detect and analyze lysozyme in hen egg white, the determined amounts of lysozyme in hen egg white were consistent with reported normal levels and recoveries were in the range of 96.0‐99.2%. After 75 consecutive runs, this prepared capillary was still stable for online preconcentration and determination of lysozyme in hen egg white without being affected by complex matrix.

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Large volume sample stacking of antiepileptic drugs in counter current electrophoresis performed in PAMAPTAC coated capillary

Cited by 17 publications

References 44 publications

Electrophoretic Determination of Symmetric and Asymmetric Dimethylarginine in Human Blood Plasma with Whole Capillary Sample Injection

Electrophoretic Determination of Symmetric and Asymmetric Dimethylarginine in Human Blood Plasma with Whole Capillary Sample Injection

Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Online preconcentration of lysozyme in hen egg white using responsive polymer coating in CE

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