Large volume syringe pump extruder for desktop 3D printers

Pusch, Kira; Hinton, Thomas J.; Feinberg, Adam W.

doi:10.1016/j.ohx.2018.02.001

Cited by 117 publications

(114 citation statements)

References 8 publications

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“…The limitation of these bioprinters is typically the cost and size. Existing 3D bioprinters tend to be large and can cost anywhere from $10,000 to over $200,000 [26], restricting widespread application of this technology. Therefore, this proof-of-concept study was intended to show the feasibility of 3D bioprinting the breast cancer microenvironment using a small low-cost 3D extrusion printer modified as an extrusion-based 3D bioprinter.…”

Section: Introductionmentioning

confidence: 99%

Bioprinted Three-Dimensional Cell-Laden Hydrogels to Evaluate Adipocyte-Breast Cancer Cell Interactions

Chaji

Al-Saleh

Gomillion

2020

Gels

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Three-dimensional (3D) bioprinting, although still in its infancy as a fabrication tool, has the potential to effectively mimic many biological environments. Cell-laden 3D printed structures have demonstrated to be an improvement from the widely used monolayer platforms, largely because of recapitulation of native tissue architecture with the 3D structures. Thus, 3D in vitro models have been increasingly investigated for improved modeling of cell and disease systems, such as for breast cancer. In the present work, multicellular cell-laden hydrogels comprised of adipocytes and breast cancer cells were bioprinted and evaluated. An ideal bioink of 3:2 5% alginate was determined to mimic the tissue stiffness observed in a physiological breast cancer tumor environment. Rheological characterization and degradation studies were performed to verify the stability of the artificial breast hydrogel environment. It was found that both the breast cancer cells and adipocytes remained viable directly after printing and throughout the 10-day culture period within the printed hydrogels. Direct printing of the cells in co-culture resulted in morphology changes and variations in cell localization within printed structures. Overall, the feasibility of efficiently fabricating multicellular cell-laden bioprinted models of the breast tumor microenvironment was established.

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Section: Introductionmentioning

confidence: 99%

Bioprinted Three-Dimensional Cell-Laden Hydrogels to Evaluate Adipocyte-Breast Cancer Cell Interactions

Chaji

Al-Saleh

Gomillion

2020

Gels

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“…129 The nature of additive manufacturing enables fabrication of certain geometries and shapes that could not be achieved through traditional methods such as mold casting. Examples include manufacture of ceramic or metallic implants (with bone-like mechanical properties and architecture) 129,130 that are customized for a patient's specific anatomy. By virtue of the materials used, such scaffolds exhibit high modulus that are comparable or greater than native bone tissue, which are well-suited for load-bearing (long bone) applications.…”

Section: Scaffold-based Approachmentioning

confidence: 99%

Calvarial Versus Long Bone: Implications for Tailoring Skeletal Tissue Engineering

Wang

Gilbert

Zhang

et al. 2020

Tissue Engineering Part B: Reviews

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Tissue-engineered graft substitutes have shown great potential to treat large bone defects. While we usually assume that therapeutic approaches developed for appendicular bone healing could be similarly translated for application in craniofacial reconstruction and vice versa, this is not necessarily accurate. In addition to those more well-known healing-associated factors, such as age, lifestyle (e.g., nutrition and smoking), preexisting disease (e.g., diabetes), medication, and poor blood supply, the developmental origins and surrounding tissue of the wound sites can largely affect the fracture healing outcome as well as designed treatments. Therefore, the strategies developed for long bone fracture repair might not be suitable or directly applicable to skull bone repair. In this review, we discuss aspects of development, healing process, structure, and tissue engineering considerations between calvarial and long bones to assist in designing the tailored bone repair strategies.

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“…Computer (AutoAnalysis 5.0 software) [9] Computer (FIALab software) [10] 6. Bioprinting, embedded printing, and food printing) 3D printers system [11] 7. Micrometal microdeposition [4] Programmable Multi Axis Controller (PMAC) [12,13] Industrial personal computer [14] 8.…”

Section: [3] Dispersive Liquid-liquid Microextractionmentioning

confidence: 99%

“…Micro/nanopatterns with micro deposition techniques have been used in various applications such as flexible electronic devices, microfluidics [1]- [4], biological tissue engineering [5], surface micromachining [6], pharmaceutical application [7]- [8], dispersive liquid-liquid microextraction [9]- [10], bioprinting sensing [11], etc. For depositing a small size of droplets that can be controlled, structured and patterned precisely is a very important process for micro fabrication.…”

Section: Introductionmentioning

confidence: 99%

Programmable Syringe Pump for Selective Micro Droplet Deposition

Kurniawan¹,

Adam

Salik³

et al. 2019

indones.j.electron.telecommun.

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Micro/nanopatterns with micro deposition techniques have been used in various applications such as flexible electronic devices, biosensing, and biological tissue engineering. For depositing a small size of droplets that can be controlled, structured and patterned precisely is a very important process for microfabrication. In this study, we developed a low cost and simple system for fabricating micro/nanostructure by a selective micro deposition process using a syringe pump. This method is an additive fabrication method where selective droplet materials are released through a needle of the syringe pump. By translating the rotating stepper motor into a linear movement of the lead screw, it will press the plunger of the syringe and give a force to the fluid inside the syringe, hence a droplet can be injected out. The syringe pump system consists of a syringe, the mechanical unit, and the controller unit. A stepper motor, the lead screw, and the mechanical components are used for the mechanical unit. Arduino Uno microcontroller is used as the controller unit and can be programmed by the computer through GUI (Graphical User Interface). The input parameters, such as the push or pull of flow direction, flow rate, the droplet volume, and syringe size dimension can be inputted by the user as their desired value via keypad or the computer. The measurement results show that the syringe pump has characteristics: the maximum average error value of the measured volume is 2.5% and the maximum average error value of the measured flow rate is 14%. The benefits of a syringe pump for micro deposition can overcome photolithography weaknesses, which require an etching and stencil process in the manufacture of semiconductors. Combining two or more syringes into one system with different droplet materials can be used as a promising method for 3D microfabrication in the future.

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Large volume syringe pump extruder for desktop 3D printers

Cited by 117 publications

References 8 publications

Bioprinted Three-Dimensional Cell-Laden Hydrogels to Evaluate Adipocyte-Breast Cancer Cell Interactions

Bioprinted Three-Dimensional Cell-Laden Hydrogels to Evaluate Adipocyte-Breast Cancer Cell Interactions

Calvarial Versus Long Bone: Implications for Tailoring Skeletal Tissue Engineering

Programmable Syringe Pump for Selective Micro Droplet Deposition

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