Large yellow croaker peroxiredoxin IV protect cells against oxidative damage and apoptosis

Wang, Xianhui; Li, Qiuhua; Mu, Pengfei; Guan, Yanyu; Chen, Xinhua; Ao, Jingqun

doi:10.1016/j.molimm.2020.08.019

Cited by 8 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, studies on the impact of stress conditions on apoptosis in L. crocea are limited. Wang et al ( 2020 ) reported on the effects of hydrogen peroxide (H 2 O 2 ) on oxidative stress and apoptosis in large yellow croaker head kidney cells and we recently found that hypoxic stress can induce apoptosis in L. crocea via the intrinsic and extrinsic pathways (unpublished data).…”

Section: Introductionmentioning

confidence: 79%

Hif-1α/Hsf1/Hsp70 signaling pathway regulates redox homeostasis and apoptosis in large yellow croaker (<i>Larimichthys crocea</i>) under environmental hypoxia

Luo

Wang

Liu

et al. 2021

Zoological Research

View full text Add to dashboard Cite

Oxygen is an essential molecule for animal respiration, growth, and survival. Unlike in terrestrial environments, contamination and climate change have led to the frequent occurrence of hypoxia in aquatic environments, thus impacting aquatic animal survival. However, the adaptative mechanisms underlying fish responses to environmental hypoxia remain largely unknown. Here, we used large yellow croaker ( Larimichthys crocea ) and large yellow croaker fry (LYCF) cells to investigate the roles of the Hif-1α/Hsf1/Hsp70 signaling pathway in the regulation of cellular redox homeostasis, and apoptosis. We confirmed that hypoxia induced the expression of Hif-1α, Hsf1, and Hsp70 in vivo and in vitro . Genetic Hsp70 knockdown/overexpression indicated that Hsp70 was required for maintaining redox homeostasis and resisting oxidative stress in LYCF cells under hypoxic stress. Hsp70 inhibited caspase-dependent intrinsic apoptosis by maintaining normal mitochondrial membrane potential, enhancing Bcl-2 mRNA and protein expression, inhibiting Bax and caspase3 mRNA expression, and suppressing caspase-3 and caspase-9 activation. Hsp70 suppressed caspase-independent intrinsic apoptosis by inhibiting nuclear translocation of apoptosis-inducing factor (AIF) and disturbed extrinsic apoptosis by inactivating caspase-8. Genetic knockdown/overexpression of Hif-1α and dual-luciferase reporter assay indicated that Hif-1α activated the Hsf1 DNA promoter and enhanced Hsf1 mRNA transcription. Hsf1 enhanced Hsp70 mRNA transcription in a similar manner. In summary, the Hif-1α/Hsf1/Hsp70 signaling pathway plays an important role in regulating redox homeostasis and anti-apoptosis in L. crocea under hypoxic stress.

show abstract

Section: Introductionmentioning

confidence: 79%

Hif-1α/Hsf1/Hsp70 signaling pathway regulates redox homeostasis and apoptosis in large yellow croaker (<i>Larimichthys crocea</i>) under environmental hypoxia

Luo

Wang

Liu

et al. 2021

Zoological Research

View full text Add to dashboard Cite

show abstract

“…under oxidative stress and in response to high H 2 O 2 levels 97-99 and apoptosis. 99 Prx is found in plants, 100 bacteria, 101 mammals 102 and other organisms. It presents several isoforms such as Prx I, II, III, 103 IV, 99 V 104 and VI.…”

Section: Peroxiredoxinmentioning

confidence: 99%

“…Peroxiredoxin (Prx; EC 1.11.1.24) is an oxidoreductase that can dismutate H 2 O 2 (Equation ) with the help of thioredoxin‐like proteins under oxidative stress and in response to high H 2 O 2 levels 97–99 and apoptosis 99 .

\mathrm{Prx}\ \left(\mathrm{reduced}\right)+{\mathrm{H}}_2{\mathrm{O}}_2\to \mathrm{Prx}\ \left(\mathrm{oxidized}\right)+2{\mathrm{H}}_2\mathrm{O}

…”

Section: Antioxidant Enzymesmentioning

confidence: 99%

See 1 more Smart Citation

Enzymes for dermatological use

Nascimento,

Torres‐Obreque,

Oliveira

et al. 2024

Experimental Dermatology

View full text Add to dashboard Cite

Skin is the ultimate barrier between body and environment and prevents water loss and penetration of pathogens and toxins. Internal and external stressors, such as ultraviolet radiation (UVR), can damage skin integrity and lead to disorders. Therefore, skin health and skin ageing are important concerns and increased research from cosmetic and pharmaceutical sectors aims to improve skin conditions and provide new anti‐ageing treatments. Biomolecules, compared to low molecular weight drugs and cosmetic ingredients, can offer high levels of specificity. Topically applied enzymes have been investigated to treat the adverse effects of sunlight, pollution and other external agents. Enzymes, with a diverse range of targets, present potential for dermatological use such as antioxidant enzymes, proteases and repairing enzymes. In this review, we discuss enzymes for dermatological applications and the challenges associated in this growing field.

show abstract

“…103 106 Moreover, PRDX4 acts as a stable ROS scavenger in a large yellow croaker head kidney cell line that stably expressed peroxiredoxin IV and protected cells from oxidative damage, apoptosis and inflammation. 107 Notably, astragalus polysaccharide, an immune enhancer in aquaculture, can substantially augment the activities of SOD and CAT in primary head kidney macrophages of large yellow croaker. Additionally, it effectively suppresses ROS generation, enhancing the antioxidant capacity of the organism.…”

Section: Lemmens Et Al Had Shown That Overexpression Of Sod1 In Mutantsmentioning

confidence: 99%

Signalling regulation of reactive oxygen species in fish inflammation

Gao,

Zhao,

Shi

et al. 2024

Reviews in Aquaculture

View full text Add to dashboard Cite

Reactive oxygen species (ROS), which are key cellular signalling molecules, are reactive chemicals containing oxygen. Cell survival or death is a critical issue in the inflammatory response. Accumulation of ROS involves ROS generation and scavenging, which determine ROS homeostasis. Understanding the roles of ROS in modulating the inflammatory response in fish is vital for helping protect fish from the damage of water pollutants in harsh environmental conditions. ROS‐related key genes and signalling pathways are relatively conserved in fish but vary among different species. Recent frequent incidences of fish diseases have posed a considerable challenge to large‐scale aquaculture. ROS is important in stress perception, integration of diverse stress‐responsive signalling networks, and activation of animal defence mechanisms, which frequently occur during inflammation in fish. This review summarises recent studies on ROS signalling pathways during inflammation in fish. Furthermore, it examines the relationship between ROS and inflammation in fish. This review may contribute to the understanding of the underlying mechanisms by which ROS regulate inflammation in fish and provide suggestions for sustainable development in aquaculture.

show abstract

Large yellow croaker peroxiredoxin IV protect cells against oxidative damage and apoptosis

Cited by 8 publications

References 41 publications

Hif-1α/Hsf1/Hsp70 signaling pathway regulates redox homeostasis and apoptosis in large yellow croaker (<i>Larimichthys crocea</i>) under environmental hypoxia

Hif-1α/Hsf1/Hsp70 signaling pathway regulates redox homeostasis and apoptosis in large yellow croaker (<i>Larimichthys crocea</i>) under environmental hypoxia

Enzymes for dermatological use

Signalling regulation of reactive oxygen species in fish inflammation

Contact Info

Product

Resources

About