“…Several families inhabit freshwaters and more than one colonization by a family is possible. Families with members that may be found in freshwater are the Achiridae (Lovejoy et al, ; Munroe, ), the Soleidae (Chapleau & Desoutter, ; Evseenko & Bolshakov, ; Munroe, ), the Cynoglossidae (Munroe, ), the Scophthalmidae (Bailly & Chanet, ; Chanet & Branellec, ), the Pleuronectidae (Munroe, ), the Paralichthyidae (Díaz de Astarloa, ; Carnikián, Acuña, & Viana, ; Cortez, Balbontín, & Landaeta, ) and the Rhombosoleidae (McDowall, ; Munroe, ). In this study, we had representatives of three families that provide the greatest number of species that inhabit estuary and freshwater environments (Munroe, ): Achiridae, Soleidae and Cynoglossidae (Appendix ).…”