Larval sex identification in the paper wasp Polistes dominulus (Vespidae, Hymenoptera)

Abstract

Identifying the sex of larvae is important in social Hymenoptera. Until now for Polistes wasps it has been necessary to genotype larvae at microsatellite loci, and assign their sex based on homozygosity at these loci. In our study on the paper wasp Polistes dominulus we have found morphological differences between larval sexes that can be used for larvae from the 3 rd instar on to easily and cheaply identify larval sex: the external gonopore and the shape and size of larval gonads. The robustness of these indicators was supported by genotype data at four microsatellite loci. Using gonopore and gonad features for sex assignment will assign diploid males as males, unlike techniques based on genetic loci or chromosomes.

Biogenic Amines and Collective Organization in a Superorganism: Neuromodulation of Social Behavior in Ants

“…Eggs produced by the queen develop into nutritionally dependent larvae that increase in size from worker provisioning, molt through a number of instars, and subsequently pupate. Each developmental stage likely has a chemical signature identifying colony identity and perhaps instar and nutritional state [Brian, 1975;den Boer and Duchateau, 2006;Cotoneschi et al, 2007]. Immatures must be maintained in temperature and humidity regimes favorable to the requirements for development of each stage, and eggs, larvae, and pupae are transported accordingly among nest chambers [Anderson and Munger, 2003].…”

Computers, Literature and Philology CLiP 2003

“…8 The text choice from the Portuguese Informal collection, with an higher MLU, represent the more typical genre in that collection, that is interviews. 9 Moreover the in all convergent positions not defined as terminal where an empty choice occurs, almost never transcribers choose a terminal sign (5 cases on 1178 positions).…”

“…8 Incluso los títulos son tratados con mucha libertad "Fingir conformidad": Ostentar rassegnazione 88a, Fingere rassegnazione 88b. 9 Vemos "atildamiento melindroso": "eleganza esagerata" 126a; "ornamento affettato" 113b. 10 Notamos también algún despiste: "zerza russa" (curiosa la creación de un doble error a partir probablemente de "berza roja"); en la edidición de 1928 "zerza rossa".…”

“…(Contini, 1970: 5). 9 Ma in termini altrettanto espliciti si era già espressa Giaveri (1993) in un contributo che ripercorre, in modo assai più sistematico e preciso di quanto si è potuto fare qui, i legami storici e teorici fra critica delle varianti italiana e critica genetica francese. 10 "[…] because the concept of a stable work and a stable text is fundamentally flawed."…”

Sociogenomics of social organization: mechanistic and evolutionary underpinnings of caste development and facial recognition in paper wasps

“…Both males and females may be produced during the late summer. We removed males from the experiment based on the patterns and coloration of the eighth and ninth abdominal segments on the thawed bodies (laboratory protocol based in part on Cotoneschi et al (2007)). Larvae were flash frozen in liquid nitrogen and stored at -80°C until RNA extraction.…”