Larval ultrastructure of Parasarcophaga dux (Thomson) (Diptera: Sarcophagidae)

Sukontason, Kom; Sukontason, Kabkaew L.; Piangjai, Somsak; Chaiwong, Tarinee; Boonchu, Noppawan; Kurahashi, Hiroki; Vogtsberger, Roy C.

doi:10.1016/j.micron.2003.07.001

Cited by 27 publications

(29 citation statements)

References 13 publications

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“…Many characteristics of the puparium of the flesh fly, L. dux (i.e., anterior spiracle, D shape of posterior spiracular disc with pronounced medial projection, three long, narrow spiracular slits oriented more or less vertically, small button below the medial projection, and lack of extensive branching of posterior spiracular hairs), all correspond with those reported in the third instar of this species using SEM (Sukontason et al 2003). However, it was discovered that the pupal respiratory horns of L. dux could not be clearly observed in this study, and tapering cavities containing convoluted structures were discovered in segments 5-11, but their function is still undetermined.…”

Section: Discussionsupporting

confidence: 73%

Surface ultrastructure of the puparia of the blow fly, Lucilia cuprina (Diptera: Calliphoridae), and flesh fly, Liosarcophaga dux (Diptera: Sarcophagidae)

et al. 2006

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Surface ultrastructure of the puparia of the blow fly, Lucilia cuprina, and flesh fly, Liosarcophaga dux, are presented utilizing scanning electron microscopy (SEM). Emphasis was focused on characteristic features of the puparia that could be used for differentiation from other forensically important fly species. The puparium of L. cuprina typically measures 6.2+/-0.2 mm in length and 2.3+/-0.1 mm in width. Each anterior spiracle contains a single row of 5-7 papillae. The intersegmental spines between the prothorax and mesothorax are triangular with constricted tips. A clustered bubble membrane, comprising about 30 mammillate structures, is positioned dorsolaterally on each side of the first abdominal segment in young puparia but is replaced by short, tubular respiratory horns in aged puparia. The posterior end of the puparium is broadly truncate and bears a pair of medially positioned posterior spiracles that each contains three straight spiracular slits. The puparium of L. dux is larger in comparison to L. cuprina and typically measures 9.9+/-0.3 mm in length and 3.8+/-0.2 mm in width. An anterior spiracle of this species contains 14-17 papillae. The intersegmental spines between the prothorax and mesothorax are broad and triangular. A convoluted structure of unknown function was observed at the dorsolateral edge of segments 5-11. In L. dux, the caudal segment of the puparium is slightly tapered and abruptly truncated and contains a pair of posterior spiracles that are located within a deep concavity in the terminal end. Each posterior spiracular disc appears D-shaped, with a pronounced medial projection and three vertically oriented long, narrow spiracular slits. The anatomical features presented herein may be useful for identification of fly puparia of these two species in future forensic entomological investigations.

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Section: Discussionsupporting

confidence: 73%

Surface ultrastructure of the puparia of the blow fly, Lucilia cuprina (Diptera: Calliphoridae), and flesh fly, Liosarcophaga dux (Diptera: Sarcophagidae)

et al. 2006

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“…Examples of such specimens from various different families include: Calliphoridae, L. cuprina (Sandeman et al 1987), Muscidae, Musca domestica (Chu-Wang and Axtell 1971), Sepsidae, numerous species (Meier 1995), Piophilidae, Piophila casei (Sukontason et al 2001a), and Sarcophagidae, P. dux (Sukontason et al 2003c). From these studies, it seems that the appearance of the dorsal organ might be a relatively constant character among species of immature flies.…”

Section: Discussionmentioning

confidence: 95%

“…This feature is in agreement with similar morphological characters described in the first instars of L. cuprina (Sandeman et al 1987), C. megacephala (Sukontason et al 2003a) and C. rufifacies (Sukontason et al 2003b), further suggesting that the morphology of these spines may help them to anchor to the substrate during locomotion, rake in food during the feeding process, and provide the ability to abrade or irritate skin resulting in myiasis (Sandeman et al 1987). However, these spines are markedly different from the pair of maxillae found in the first instars of the bot fly, Dermatobia hominis (de Filippis and Leite 1997) or flesh fly, Parasarcophaga dux (Sukontason et al 2003c). The tripartite labium observed in the first instar of H. ligurriens is virtually identical to those labia seen in third instars of other blow fly species, such as L. cuprina (Sandeman et al 1987) and C. rufifacies (Sukontason et al 2003b).…”

Section: Discussionmentioning

confidence: 99%

Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications

et al. 2008

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In forensic investigations, all immature stages of flies (egg, larvae, and puparium) can serve as entomological evidence at death scenes. These insects are primarily used to estimate the post mortem interval (PMI), but can also be involved in the analysis of toxic substances, determining manner of death, and in indicating relocation of a corpse in homicide cases. In this study, we present the morphology of the egg, larvae, and puparium of Hemipyrellia ligurriens, a blow fly species of forensic importance in Thailand. Examination was conducted using scanning electron microscopy (SEM). The egg stage was found to display a relatively wide plastron region (or median hatch line area) that spans almost the entire length of the egg. The median hatch line is oriented in an upright position. External chorionic sculpture of the egg is present in a hexagonal pattern whose reticular boundaries are slightly elevated. In the larval stages, the most prominent morphological changes were detected upon comparison of the first to the second instar; whereas, the differences between second and third instar larvae were less obvious outside of the increase in number of posterior spiracular slits. Most of the major differences involve body size and structure of the anterior and posterior spiracles. Each anterior spiracle in both the second and third instars projects five to seven papillae apically. Each posterior spiracular disc of a third instar exhibits a complete peritreme, three spiracular slits, and a prominent button that is ventromedially located. The puparium is coarctate and features a clustered bubble membrane comprised of approximately 57 mammillate structures positioned dorsolaterally on each side of the first abdominal segment in young puparia. This feature is replaced by short, tubular respiratory horns in aged puparia. This study provides more detailed exposure of important morphological features that can be used for accurate identification of immature stages of H. ligurriens. Information presented can aid in forensic investigations involving this fly species.

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“…Life cycle of Sarcophaga dux from the first instar to the adult was 312.0 ±3.0 h under uncontrolled indoor temperature in Malaysia [26]. Report from study in north Thailand in the year 2002-2003 under natural temperature indicates seasonal variation in development of fly with rapid larval development in summer at around 72 hrs, while it took 72-96 hrs.in rainy season and 96 hrs.in winter [27][28]. The life cycle of Sarcophaga cultellata at 25 0 C and 50% humidity showed a total development time from larvae position to first adult emergence was 330 ± 12 hr [29].…”

Section: Discussion:-mentioning

confidence: 99%

Effect of Seasonal Temperature Variation on the Duration of Life Cycle Stages of the Fly of Forensic Importance, Parasarcophga Dux (Thomson) (Diptera: Sarcophagidae).

Siddiki¹,

Sp²

2017

IJAR

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Sarcophagid flies belonging to carrion insect communities provide the information about the corps. Parasarcophaga dux (Thomson) (Diptera: Sarcophagidae) collected from Latur district of Maharashtra (India) has been studied for the duration of different life cycle stages in three different seasons i.e. rainy, winter and summer seasons under the room temperature. The female released I st instar larvae which after two larval mounts undergo prepupation and then pupation in the soil. The adult emerged out from pupae. During rainy season the life cycle duration from larvaeposite to emergence of adult was 315.15 ± 3.28 hrs, during winter season in 359.56 ± 2.44 hrs, while in summer season it took 265.51 ± 6.28 hrs, under the fluctuating diurnal temperature and humidity of the laboratory. The time durations of life cycle stages of P. dux in the different seasons can be used to determine the PMI using the meterological data and P. dux life cycle stages collected from the cadaver.

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Larval ultrastructure of Parasarcophaga dux (Thomson) (Diptera: Sarcophagidae)

Cited by 27 publications

References 13 publications

Surface ultrastructure of the puparia of the blow fly, Lucilia cuprina (Diptera: Calliphoridae), and flesh fly, Liosarcophaga dux (Diptera: Sarcophagidae)

Surface ultrastructure of the puparia of the blow fly, Lucilia cuprina (Diptera: Calliphoridae), and flesh fly, Liosarcophaga dux (Diptera: Sarcophagidae)

Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications

Effect of Seasonal Temperature Variation on the Duration of Life Cycle Stages of the Fly of Forensic Importance, Parasarcophga Dux (Thomson) (Diptera: Sarcophagidae).

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