Larvicidal activity of granulated pharmaceutical products using Indonesian holy basil leaf extract

Ikhsanudin, Azis; Lolita, Lolita; Ramadani, Zayyana Septya

doi:10.11591/ijphs.v10i4.21004

Cited by 4 publications

(6 citation statements)

References 29 publications

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“…aegypti yang berbeda bila dibuat larutan atau ekstrak. 18 Pelarut etanol digunakan dalam penelitian ini karena bersifat polar sehingga mampu mengekstraksi senyawa fenolik. Etanol memiliki kelebihan mampu menemukan senyawa kimia lebih banyak dibandingkan dengan methanol dan air.…”

Section: Pembahasanunclassified

Efektivitas Ekstrak Daun Kemangi (Ocimum sanctum Linn) Terhadap Mortalitas Larva Aedes aegypti

Tamtama

Wardani²,

Almanfaluthi³

et al. 2023

blb

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Basil leaf (Ocimum sanctum Linn) are known to have the ability as natural larvicides because there are various compounds including tannins, eugenol, flavonoids, essential oils, hexauronic acid, saponins, pentose, xylose, metal homocytic acid, mulludistin, ursolic acid and methyl clavical. This study aims to determine the effectiveness of basil leaf extract (O. sanctum Linn) on mortality of Aedes agypti larvae. This research is a true experimental type with posttest only with control group design. Aedes aegypti larvae obtained from Balitbangkes Banjarnegara were used in this study as many as 450 larvae. This study consisted of 6 groups with 2 control groups (positive control/temefos 1% and negative control/distilled water) and 4 other groups receiving treatment with basil leaf extract concentrations of 1.5%, 1.8%, 2.1%, and 2.4%. Each group contained 25 larvae of Ae. aegypti with three repetitions and observation time with 10th, 40th, 60th, 120th, 240, 720, and 1440 minute intervals. Larval mortality of Ae. aegypti occurred from the 120th minute with a concentration of 2.4% with a mortality percentage of 5.6%. The results of the Kruskal Wallis test showed that there were differences in the mortality of Ae. aegypti at 60, 120, 240, 720, and 1440 minutes. The LC50 value was 2.329% at 720 minutes while the LC90 test was 7.043% at 720 minutes. Basil leaf extract has not been effective as a larvicide because mortality occurred from the 120th minute.

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Section: Pembahasanunclassified

Efektivitas Ekstrak Daun Kemangi (Ocimum sanctum Linn) Terhadap Mortalitas Larva Aedes aegypti

Tamtama

Wardani²,

Almanfaluthi³

et al. 2023

blb

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“…Waktu dispersi yang lebih pendek dapat menghasilkan lebih banyak zat aktif dalam air sebagai medium penggunaan granul, yang akan mempengaruhi aktivitas larvasida. 23 Waktu terdispersi granul yang baik kurang dari 5 menit. Sudut diam menggambarkan sifat aliran granul.…”

Section: Tabel 3 Aktivitas Larvasida Ekstrak Dan Granul Daun Komba-kombaunclassified

“…Amilum juga dapat memfasilitasi pengangkutan cairan ke dalam pori-pori granul untuk meningkatkan penyerapan zat cair yang memasuki pori-pori granul. 23 Hasil pengujian menunjukkan GET memenuhi semua parameter standar stabilitas fisik yang diujikan.…”

Section: Tabel 3 Aktivitas Larvasida Ekstrak Dan Granul Daun Komba-kombaunclassified

Ujı aktıvıtas sedıaan granul darı ekstrak etanol daun komba-komba (Chromolaena odorata L.) sebagaı larvasıda

Armadany

Solo

Utama

et al. 2022

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Komba-komba (Chromolaena odorata L.) merupakan salah satu tumbuhan yang dapat digunakan sebagai larvasida alami. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak daun komba-komba berbunga kuning dan bentuk sediaan granulnya sebagai larvasida. Daun komba-komba diekstraksi secara maserasi menggunakan etanol 96% kemudian didelipidasi menggunakan n-heksan. Ekstrak diidentifikasi metabolit sekundernya melalui skrining fitokimia secara kualitatif mengikuti metode Harborne. Pengujian aktivitas larvasida dilakukan pada ekstrak etanol dan ekstrak etanol terdelipidasi pada konsentrasi 250 ppm, 500 ppm, 1000 ppm dan 2000 ppm untuk mengetahui nilai Lethal Concentration/LC50. Ekstrak diformulasi menjadi sediaan granul menggunakan metode granulasi basah. Hasil skrining fitokimia menunjukkan metabolit sekunder ekstrak etanol daun komba-komba yaitu alkaloid, flavonoid, saponin dan tannin. Uji aktivitas larvasida menunjukkan bahwa ekstrak etanol terdelipidasi memiliki aktivitas lebih baik dibandingkan ekstrak etanol dengan nilai LC50 ekstrak etanol terdelipidasi sebesar 261,6 ppm dan ekstrak etanol sebesar 317,1 ppm. Ekstrak tanol terdelipidasi dengan konsentrasi 1000 ppm ke atas berpotensi untuk dikembangkan dalam bentuk sediaan granul karena memiliki aktivitas larvasida setara dengan kontrol positif (temefos 1000 ppm). Ekstrak etanol terdelipidasi daun komba-komba diformulasikan dalam bentuk sediaan granul menggunakan kombinasi eksipien amilum, PVP dan laktosa. Uji aktivitas larvasida pada granul menunjukkan nilai LC50 sebesar 225,8 ppm.

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“…In another study, treatment with granu-lated formulations of O. tenuiflorum led to up to 90% mortality of the Ae. aegypti larvae at 6000 ppm [26].…”

Section: Introductionmentioning

confidence: 99%

Larvicidal Activity and Phytochemical Profiling of Sweet Basil (Ocimum basilicum L.) Leaf Extract against Asian Tiger Mosquito (Aedes albopictus)

Chan

Sit

2022

Horticulturae

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Applying larvicides to interrupt a mosquito’s life cycle is an important strategy for vector control. This study was conducted to evaluate the larvicidal properties of the hexane extract of sweet basil (Ocimum basilicum L.; family Lamiaceae) leaves against the wild strain of Asian tiger mosquito, Aedes albopictus (Skuse). Third instar larvae (20 larvae/replicate, n = 3) were exposed to different concentrations of the extract (6.25–200 µg/mL), and the mortality rate was recorded. Probit analysis showed that the median lethal concentration and 95% lethal concentration of the extract were 16.0 (10.9–22.1) and 53.0 (34.6–136.8) µg/mL, respectively, after 24 h exposure. Only the fractions F3, F4, and F5 from the column chromatography displayed high mortality rates of 91.7–100% at 25.0 µg/mL after 24 h exposure. Subsequent column chromatography from the pooled fraction yielded two active subfractions, H-F345-S2 and H-F345-S3, with mortality rates of 100% and 98.3 ± 2.9%, respectively, at 12.5 µg/mL. Gas chromatography–mass spectrometry analysis unveiled that methyl chavicol, 2-(2-butoxyethoxy)ethanol, cedrelanol, methyl eugenol, 2,4,di-tert-butylphenol, and phytol were the major components in both subfractions with some of them being reported as larvicidal compounds. The results suggest that sweet basil has substantial larvicidal activity against Ae. albopictus mosquito and is a potential source of naturally derived larvicide.

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Larvicidal activity of granulated pharmaceutical products using Indonesian holy basil leaf extract

Cited by 4 publications

References 29 publications

Efektivitas Ekstrak Daun Kemangi (Ocimum sanctum Linn) Terhadap Mortalitas Larva Aedes aegypti

Efektivitas Ekstrak Daun Kemangi (Ocimum sanctum Linn) Terhadap Mortalitas Larva Aedes aegypti

Ujı aktıvıtas sedıaan granul darı ekstrak etanol daun komba-komba (Chromolaena odorata L.) sebagaı larvasıda

Larvicidal Activity and Phytochemical Profiling of Sweet Basil (Ocimum basilicum L.) Leaf Extract against Asian Tiger Mosquito (Aedes albopictus)

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