Larvicidal and irritant activities of hexane leaf extracts of Citrus sinensis against dengue vector Aedes aegypti L.

Warikoo, Radhika; Ray, Ankita; Sandhu, Jasdeep Kaur; Samal, Roopa Rani; Wahab, Naim; Kumar, Sarita

doi:10.1016/s2221-1691(11)60211-6

Cited by 48 publications

(33 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results confirm our preparation as an active larvicide, with a reproducible mode of delivery [30][31][32]. This is particularly important due to the high variation in cidal activity of orange oil described in previous studies, including variations in LD 50 from < 100 mg/l against mosquito larvae [5,7,30,33] to a complete lack of larvicidal activity [6,8,34]. In some studies, these differences may be attributed to the methodology used for extraction of the EO, as in the case of larvicidal activities reported for hexane extracts of Citrus limetta compared to petroleum ether extracts of the same plant [35].…”

Section: Discussionsupporting

confidence: 82%

“…7 Department of General Preventive Medicine, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA. 8 Loyola University Stritch School of Medicine, Maywood, IL, USA.…”

Section: Abbreviationsmentioning

confidence: 99%

“…In recent years, larvicidal activities of EOs from a number of plants [4,5], including Citrus essential oils [6][7][8][9], Cryptomeria japonica (Japanese cedar) [10], Lippia sidoides (pepper-rosmarin) [11], Cinnamomum osmophloeum (cinnamon) [12], Syzygium aromaticum (clove) [13] and Cymbopogon citratus (lemongrass) [13,14] have been reported. EOs have been shown to exert larvicidal effects through at least three different mechanisms: neurotoxicity [15,16], growth inhibition, and interruption of metabolic pathways [17][18][19].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Yeast-encapsulated essential oils: a new perspective as an environmentally friendly larvicide

et al. 2020

View full text Add to dashboard Cite

Background: Effective mosquito control approaches incorporate both adult and larval stages. For the latter, physical, biological, and chemical control have been used with varying results. Successful control of larvae has been demonstrated using larvicides including insect growth regulators, e.g. the organophosphate temephos, as well as various entomopathogenic microbial species. However, a variety of health and environmental issues are associated with some of these. Laboratory trials of essential oils (EO) have established the larvicidal activity of these substances, but there are currently no commercially available EO-based larvicides. Here we report on the development of a new approach to mosquito larval control using a novel, yeast-based delivery system for EO. Methods: Food-grade orange oil (OO) was encapsulated into yeast cells following an established protocol. To prevent environmental contamination, a proprietary washing strategy was developed to remove excess EO that is adsorbed to the cell exterior during the encapsulation process. The OO-loaded yeast particles were then characterized for OO loading, and tested for efficacy against Aedes aegypti larvae. Results: The composition of encapsulated OO extracted from the yeast microparticles was demonstrated not to differ from that of un-encapsulated EO when analyzed by high performance liquid chromatography. After lyophilization, the oil in the larvicide comprised 26-30 percentage weight (wt%), and is consistent with the 60-65% reduction in weight observed after the drying process. Quantitative bioassays carried with Liverpool and Rockefeller Ae. aegypti strains in three different laboratories presented LD 50 of 5.1 (95% CI: 4.6-5.6) to 27.6 (95% CI: 26.4-28.8) mg/l, for L1 and L3/L4 mosquito larvae, respectively. LD 90 ranged between 18.9 (95% CI: 16.4-21.7) mg/l (L1 larvae) to 76.7 (95% CI: 69.7-84.3) mg/l (L3/L4 larvae). Conclusions: The larvicide based on OO encapsulated in yeast was shown to be highly active (LD 50 < 50 mg/l) against all larval stages of Ae. aegypti. These results demonstrate its potential for incorporation in an integrated approach to larval source management of Ae. aegypti. This novel approach can enable development of affordable control strategies that may have significant impact on global health.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Abbreviationsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Yeast-encapsulated essential oils: a new perspective as an environmentally friendly larvicide

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The eggs were collected in an ovitrap lined with Whatman filter paper strips which were then transferred into the enamel trays filled with at least 1.5-2.0 L of dechlorinated water. The hatched larvae were fed on powdered dog biscuits and yeast in a ratio of 3:1 [26]. The pupae were collected on regular basis and were kept in clothed cages for adult emergence.…”

Section: Culture Of Mosquitoesmentioning

confidence: 99%

Susceptibility Status of Aedes aegypti L. Against Different Classes of Insecticides in New Delhi, India to Formulate Mosquito Control Strategy in Fields

Samal¹,

Kumar²

2018

TOPARAJ

Self Cite

View full text Add to dashboard Cite

Background: Mosquito control is a major concern throughout the world because of rising cases of mosquito-borne diseases. The outbreak of Zika, Dengue and Chikungunya has caused grave situations raising urgent need to control Aedes aegypti. Moreover, extensive use of synthetic insecticides in mosquito control programs has resulted in high levels of insecticide resistance leading to the use of magnified concentrations, impacting human health and environment adversely. The knowledge about current status of the insecticide susceptibility against Ae. aegypti could help to devise mosquito control strategy. Objective: Present study evaluates the larvicidal potential of thirteen insecticides belonging to seven different classes; organochlorines, organophosphates, carbamates, pyrethroids, neonicotinoids, avermectins and secondary metabolites; against early fourth instars of Ae. aegypti. Materials and Methods: The insecticide susceptibility was evaluated as per WHO protocol. Fatality counts were made after 24h of exposure; and the LC50, LC90 and other statistical parameters were computed by probit-regression analysis. Results: The data reveals the maximum efficacy of pyrethroids and fenitrothion, with lethal values less than 0.001 ppm. Avermectins, organochlorines and carbamates were moderately toxic, while neonicotinoid posed appreciable toxicity. In contrast, berberine, a secondary plant metabolite was found inefficient. The larvicidal efficacy of tested insecticides against Ae. aegypti was found in the decreasing order of pyrethroids > organophosphates > avermectins > organochlorines > carbamates > neonicotinoids > secondary metabolites. Conclusion: Present investigations explore various toxicants as Dengue vector control agents in order to devise a suitable control strategy for mosquito control in fields.

show abstract

“…7,8,9,10 Leaf extracts have shown antioxidant, antifungal, anti-Helicobacter pylori and larvicidal activity against the dengue vector and negative ionotropic activities in experimental studies. 11,12,13,14,15 The leaf oil has insecticidal activity against larvae of Culex pipiens molestus mosquito. 16 Citrus paradisi (Macf.)…”

Section: Introductionmentioning

confidence: 99%

Comparative Pharmacognostic Evaluation of Leaves of Citrus sinensis Var. Jaffa and Citrus paradisi var. Redblush

Sohi¹,

Shri²

2018

View full text Add to dashboard Cite

Background:The genus Citrus (family: Rutaceae), a native to South East Asia and China, comprises aromatic shrubs and trees which are highly acknowledged by folk medicine of various tropical tribes and cultures and also by modern science for the treatment of various ailments. Citrus sinensis var. jaffa and Citrus paradisi var. redblush are two medicinally important members of this genus. However, the first step in utilization of a medicinal plant is authentication which involves pharmacognostic examination of the plant material. Systematic pharmacognostic studies for leaves of these Citrus species have not yet been done. Objective: To study and compare pharmacognostic profile of leaves of Citrus sinensis var. jaffa and Citrus paradisi var. redblush. Methods: Leaves were subjected to detailed macroscopic, microscopic (qualitative and quantitative), physiochemical, fluorescence and preliminary phytochemical analysis as per standard pharmacopoeial procedures and WHO guidelines. Results: Macroscopic examination showed that leaves of the two species can be differentiated based on nature of petiole, size and shape. Diagnostic microscopic features including the size of the epidermal cells, the type of stomata, stomatal index and location of secretory cavities help to distinguish the two species. Results for physiochemical and fluorescence analysis were recorded which will serve as reference standards. Preliminary phytochemical screening showed the presence of alkaloids, flavonoids, tannins, saponins, steroids, volatile oils, proteins and sugars. Conclusion: Pharmacognostic standards for leaves have been generated for the first time. These may prove useful to establish identity, quality and purity of these medicinally important Citrus species.

show abstract

Larvicidal and irritant activities of hexane leaf extracts of Citrus sinensis against dengue vector Aedes aegypti L.

Cited by 48 publications

References 26 publications

Yeast-encapsulated essential oils: a new perspective as an environmentally friendly larvicide

Yeast-encapsulated essential oils: a new perspective as an environmentally friendly larvicide

Susceptibility Status of Aedes aegypti L. Against Different Classes of Insecticides in New Delhi, India to Formulate Mosquito Control Strategy in Fields

Comparative Pharmacognostic Evaluation of Leaves of Citrus sinensis Var. Jaffa and Citrus paradisi var. Redblush

Contact Info

Product

Resources

About