Larvicidal, biological, and histopathological alterations induced by pomegranate peel extract, Punica granatum against Culex pipiens L. (Diptera: Culicidae)

Abstract

Dipterous insects cause serious public health problems for both humans and animals (Linthicum, 2012). In Egypt, Culex pipiens (Diptera: Culicidae), has been investigated and declared as a vector of several diseases (El-Zayyat et al., 2017). It transmits Rift valley fever virus (Dodson et al., 2017), Japanese encephalitis (Chancey et al., 2015), Wuchereria bancrofti accredited for human lymphatic filariasis transmission (Joseph et al., 2011), and West Nile virus (Bassal et al., 2017). Culex pipiens was incriminated as the filarial vector in Egypt (El-Naggar et al., 2017) and has been recorded by all governorates without exception (Abdel-Shafi et al., 2016). Traditional insecticides were used to control mosquitoes (Killeen et al., 2017) but introduced problems in the environment by leaving undesirable residues in food, toxicological implications to human health, increased cost and handling hazards (Bonner and Alvanja, 2017). Flies have developed a certain level of resistance to most available insecticides ARTICLE INFO

Phytochemical Investigation of Three Cystoseira Species and Their Larvicidal Activity Supported with In Silico Studies

“…All tested extracts showed a significant reduction in protein, lipids, and carbohydrate contents of treated larvae compared to untreated larvae, which may impair the survival and development of the larvae. Our results were in accordance with previous reports that reported that larvicides have a deleterious impact on larval growth and development, causing alterations in their metabolic and biochemical processes [ 62 ].…”

Anti‐Culex pipiens activity of different pomegranate cultivars and determination of their bioactive compounds using LC‐MS profiling

“…Previous studies reported that the ethanol extract of pomegranate fruits exhibited larvicidal activity against C. pipiens 17 . In addition, larvicidal activity against C. pipiens was reported for the acetone and petroleum ether extracts of pomegranate peels 44,45 . Interestingly, the methanol extract of pomegranate rind exhibited the highest toxicity on the fourth instar larvae of Culex quinquefasciatus and Cricotopus gelidus when compared to other extracts (hexane and ethyl acetate) 46 .…”

“…42 In this regard, the metabolic profiles of seven petroleum ether extracts of pomegranate peels. 44,45 Interestingly, the methanol extract of pomegranate rind exhibited the highest toxicity on the fourth instar larvae of Culex quinquefasciatus and Cricotopus gelidus when compared to other extracts (hexane and ethyl acetate). 46 Therefore, in this study several concentrations of the peel methanol extracts of seven pomegranate cultivars were evaluated against newly molted third instar C. pipiens larvae and their larvicidal effects were determined.…”

“…Mortality rates, LC 50 , the toxicity index, and relative potency values of peel extracts are provided in Larvicidal activity of pomegranate extracts may be attributed to the phenolic groups contained in the tannins. 45 Tannins inhibit cell protein synthesis through the irreversible formation of a complex with cellular proteins. 48 Furthermore, many studies have demonstrated the crucial role of alkaloids in controlling mosquito larvae.…”

The Proficiency of Silver Nanoparticles in Controlling Cotton Leafworm, Spodoptera Littoralis (Boisd.), Under the Laboratory Conditions

“…In addition, in treated larvae of Tuta absoluta with phenthoate, imidacloprid, and dinotefuran, Radwan and Taha [48] found a correlation between the loss of various proteins and the reduction of band intensity when using SDS PAGE. In addition, Farag et al [49] observed the disappearance of normal protein bands in the treated Cx. pipiens larvae with pomegranate peel extracts.…”