Larvicidal, Histopathological Efficacy of Penicillium daleae against Larvae of Culex quinquefasciatus and Aedes aegypti Plus Biotoxicity on Artemia nauplii a Non-target Aquatic Organism

Ragavendran, Chinnasamy; Mariappan, T

doi:10.3389/fphar.2017.00773

Cited by 41 publications

(29 citation statements)

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“…Many histological changes in most insects due to the infection with different entomopathogenic fungi were revealed using light and electron microscopy by many investigators (Scheneider et al, 2013;Gabarty et al, 2014;Khaleil et al, 2016;Ragavendran et al, 2017). The insects cuticle, with its underlying epidermis, forms the integument.…”

Section: Results Of Histological Studymentioning

confidence: 99%

Histological changes in the larvae of the domestic mosquito Culex pipiens treated with the entomopathogenic fungus Beauveria bassiana

Farida¹,

Sonia²,

Hakima³

et al. 2018

Sci. Res. Essays

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The Culicidae are biting insects that are most harmful to people. They are almost all blood-suckers, and are responsible for the spread of many important diseases such as malaria, yellow fever and elephantiasis. Entomopathogenic microorganisms occupy an important place among the alternative methods of fighting against pests insect. The fungus, Beauveria bassiana is an entomopathogenic agent naturally present in the ecosystems and offers a very interesting potential for controlling populations of mosquitoes. Our study aims at showing the 4 th stage larvae histological changes of the domestic mosquito Culex pipiens, treated with the entomopathogenic fungus B. bassiana in suspension which corresponds to a dose of 10 7 spore/mL. In fact, after developing the individuals in the fungal solution, histological study was carried out on the following parts; cuticle, intestine, adipose tissue and hemolymph. The histological studies showed many alterations and malformation in the treated 4 th instar larvae body and tissues. The infection affected the different parts of the body, especially the cuticle, the adipose cells, and midgut, in addition to the development and the colonization of B. bassiana inside tissues. The application of B. bassiana on the cuticle of the larvae was dependent on an apparent disturbance in the structure of the cuticle or the degeneration of its different parts. The fungus infection does not stop at the level of the body walls, so it even goes to adipose tissue, epidermal cells and intestine. Based on these results, B. bassiana could be suggested as suitable biocontrol agent against C. pipiens.

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Section: Results Of Histological Studymentioning

confidence: 99%

Histological changes in the larvae of the domestic mosquito Culex pipiens treated with the entomopathogenic fungus Beauveria bassiana

Farida¹,

Sonia²,

Hakima³

et al. 2018

Sci. Res. Essays

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“…quinquefasciatus. Recently, Ragavendran et al (2017) reported that the midgut cells of Ae. aegypti (fourth-instar larvae) had swelling in the gut lumen, reducing intercellular contacts and degeneration of nuclei, after treated with P. daleae mycelium extract.…”

Section: Discussionmentioning

confidence: 99%

“…The present investigation reveals that the uptake with A. terreus mycelium ethyl acetate extract treated with A. nauplii shown an accumulation of toxin in the mid-gut region leads to the brine shrimp death. Recently, Ragavendran et al (2017) identified Penicillium daleae mycelia metabolites and tested for A. nauplii alterations of body structures and loss of antenna with the LC 50 and LC 90 value 0.290 and 0.609 μg/ml, respectively. Similarly, Ruhul Amin et al (2003) reported the LC 50 value (17.78 μg/ml) of Penicillium sp.…”

Section: Discussionmentioning

confidence: 99%

“…The evaluation of mortality and swimming speed alteration of non-target organism ( A. nauplii ) was assessed by the modified methods of Ragavendran et al (2017) and Benelli et al (2018) . Five different concentrations of extract (100, 200, 300, 400, and 500 μg/ml) were used for this study.…”

Section: Methodsmentioning

confidence: 99%

“…A. terreus acts as a biocontrol agent against Biomphalaria alexandrina snails ( Saad et al, 2014 ), and promoter for plant growth, and adverse effects of stem rot disease caused by Sclerotium rolfsii ( Waqas et al, 2015 ). The toxicity of the soil-borne fungal extracts from Aspergillus species ( A. flavus, A. niger , and A. parasiticus ) ( Maurya et al, 2011 ), Beauveria tenella ( Balaraman et al, 1979 ), B. bassiana ( Ragavendran et al, 2017 ), Lagenidium giganteum ( Lacey et al, 1988 ; Vyas et al, 2007 ), and Chrysosporium lobatum ( Mohanty and Prakash, 2008 ) are widely reported as against mosquito larvae. Earlier, Ravindranath and Kapadnis (1991) investigated the isolation of Fusarium oxysporum and Fusarium pallidoroseum from mosquito larvae, and test their potential against stages of mosquito larvae.…”

Section: Introductionmentioning

confidence: 99%

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Aspergillus terreus (Trichocomaceae): A Natural, Eco-Friendly Mycoinsecticide for Control of Malaria, Filariasis, Dengue Vectors and Its Toxicity Assessment Against an Aquatic Model Organism Artemia nauplii

2018

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Vector-borne diseases like malaria, filariasis, and dengue are transmitted by mosquitoes and they cause global mortality and morbidity due to an increased resistance against commercial insecticides. The present study was aimed to evaluate the neurobehavioral toxicity, knock-down effect, histopathology, ovicidal, adulticidal, and smoke toxicity effect of Aspergillus terreus extract against three mosquito species, namely Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti (Diptera: Culicidae). The isolated fungal strain was identified as A. terreus (GenBank accession no: KX694148.1) through morphological and molecular (phylogenetic) analysis. The morphological changes in the treated fourth instar larvae shown the demelanization of cuticle and shrinkage of the internal cuticle of anal papillae. The time duration of extract exposure against the larvae determines the level of toxicity. The extract treated larvae were displayed excitation, violent vertical and horizontal movements with aggressive anal biting behavior as the toxic effect on the neuromuscular system. The results of the biochemical analysis indicated that a decrease in the level of acetylcholinesterase, α-carboxylesterase, and β-carboxylesterase in extract treated fourth instar larvae of all tested mosquito species. The findings of histopathological investigation shown the disorganization of the abdominal region, mainly in mid, hindgut, and gastric caeca, loss of antenna, lateral hair, caudal hair, upper and lower head hairs in the mycelium extract treated An. stephensi, Cx. quinquefasciatus, and Ae. aegypti. The ovicidal bioassay test results showed the mosquito hatchability percentage was directly related to the concentrations of mycelium extract. Nil hatchability of mosquito eggs was noticed at 500 μg/ml concentration. The adulticidal activity of fungal mycelia ethyl acetate extract resulted in a dose-dependent activity (15 and 30 min recovery periods). The higher concentration of extract (1000 mg/L) acted as a repellent, the adult mosquitoes showed restless movement, uncontrolled/anesthetic flight at last died. The better adulticidal activity was observed in the ethyl acetate extract against An. stephensi, Cx. quinquefasciatus followed by Ae. aegypti with the best score of LD50 and LD90 values and nil mortality was found in the control. The results of smoke toxicity assay of the mycelia extract exhibited significant mortality rate against Ae. aegypti (91%), Cx. quinquefasciatus (89%), and An. stephensi (84%). In addition, the present investigation reported the stability and toxic effects of A. terreus mycelium ethyl acetate extract on Artemia nauplii. The swimming speed (0.88 mm s-1) of A. terreus was reduced with ethyl extract 24 h treatment whereas, the control A. nauplii showed the normal speed of 2.96 mm s-1. Altered behavior and swimming movement were observed in the 8 h A. terreus mycelium extract treated A. nauplii. A pale yellow color substance (metabolites) was found in the mid-gut region of the mycelial extract exposed A. nauplii....

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Larvicidal and histopathological efficacy of inhabitant pathogenic bacterial strains to reduce the dengue vector competence

Rajagopal¹,

Jeyavani²,

Ilango³

2020

Pest Management Science

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BACKGROUND: Aedes aegypti is a primary vector of dengue virus, and the causative agent of dengue is emerging globally as one of the most important arboviral diseases currently threatening human populations. Therefore, vector control is presently the primary intervention method of population reduction, in which natural A. aegypti populations would be reduced with inhabitant bacterial strains that are unable to transmit dengue virus. RESULT: Based on the pathogenicity of strains, only four isolates effectively show larvicidal activity. The 16S rRNA gene sequences and the phylogeny depicted that the potential isolates were Bacillus firmus (MK791255), Bacillus paramycoides (MK788268), Bacillus siamensis (MK788212), and Bacillus licheniformis (MK791256). After 24 and 48 hours exposure, the B. licheniformis strain (cell mass of 2.2 × 10 7 CFU mL-1) showed potent larvicidal activity with LC 50 of 16.22 ∼g mL-1 and 9.57 ∼g mL-1 and the B. paramycoides (cell mass of 3.1 × 10 7 CFU mL-1) strain inhibits the larval and pupal development with LC 50 of 42.62 ∼g mL-1 and 26.97 ∼g mL-1. Intermittent stages and causes of abscess in the gut and siphon regions were observed through histopathological studies. These two bacterial strains extend larval duration up to 15-16 days as well as reduce development. CONCLUSION: These studies demonstrate the challenge for dengue vector in reducing developmental and reproduction competence.

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Larvicidal, Histopathological Efficacy of Penicillium daleae against Larvae of Culex quinquefasciatus and Aedes aegypti Plus Biotoxicity on Artemia nauplii a Non-target Aquatic Organism

Cited by 41 publications

References 77 publications

Histological changes in the larvae of the domestic mosquito Culex pipiens treated with the entomopathogenic fungus Beauveria bassiana

Histological changes in the larvae of the domestic mosquito Culex pipiens treated with the entomopathogenic fungus Beauveria bassiana

Aspergillus terreus (Trichocomaceae): A Natural, Eco-Friendly Mycoinsecticide for Control of Malaria, Filariasis, Dengue Vectors and Its Toxicity Assessment Against an Aquatic Model Organism Artemia nauplii

Larvicidal and histopathological efficacy of inhabitant pathogenic bacterial strains to reduce the dengue vector competence

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