Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study

Karakaidos, Panagiotis; Kryou, Christina; Simigdala, Nikiana; Klinakis, Apostolos; Zergioti, I.

doi:10.3390/bioengineering9080378

Cited by 16 publications

(9 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The low energy of photons with λ = 1053 nm and the relatively long duration of laser pulses (τ < 7 ns), which do not provide multiphoton absorption, do not lead to the direct destruction of chemical bonds [23]. The studies carried out in [24] showed that even the use of shorter wavelength ultraviolet radiation of 355 nm with a pulse duration of 10 ns (approximately as in our work) leads to negligible genotoxic stress during laser bioprinting. Two optical elements-a telescopic lens with a magnification range of 2×-8× and a motorized beam expander with a magnification range of 1×-3× (Optogama, Vilnius, Lithuania) were used to adjust the beam diameter and change its divergence.…”

Section: Experimental Setup For Bioprinting With Cell Spheroidssupporting

confidence: 54%

Laser Bioprinting with Cell Spheroids: Accurate and Gentle

et al. 2023

View full text Add to dashboard Cite

Laser printing with cell spheroids can become a promising approach in tissue engineering and regenerative medicine. However, the use of standard laser bioprinters for this purpose is not optimal as they are optimized for transferring smaller objects, such as cells and microorganisms. The use of standard laser systems and protocols for the transfer of cell spheroids leads either to their destruction or to a significant deterioration in the quality of bioprinting. The possibilities of cell spheroids printing by laser-induced forward transfer in a gentle mode, which ensures good cell survival ~80% without damage and burns, were demonstrated. The proposed method showed a high spatial resolution of laser printing of cell spheroid geometric structures at the level of 62 ± 33 µm, which is significantly less than the size of the cell spheroid itself. The experiments were performed on a laboratory laser bioprinter with a sterile zone, which was supplemented with a new optical part based on the Pi-Shaper element, which allows for forming laser spots with different non-Gaussian intensity distributions. It is shown that laser spots with an intensity distribution profile of the “Two rings” type (close to Π-shaped) and a size comparable to a spheroid are optimal. To select the operating parameters of laser exposure, spheroid phantoms made of a photocurable resin and spheroids made from human umbilical cord mesenchymal stromal cells were used.

show abstract

Section: Experimental Setup For Bioprinting With Cell Spheroidssupporting

confidence: 54%

Laser Bioprinting with Cell Spheroids: Accurate and Gentle

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Lastly, stereolithography involves polymerisation of lightsensitive polymers by finely controlled light projected from digital nano-mirrors [29] . It offers low-cost but high quality (resolution of 100 µm), speed, and 90% cell viability [30][31] . It's unlimited by viscosity (1-300 mPa/s) [32] , but its use of ultraviolet light has been shown to damage cell DNA [30][31] .…”

Section: Printing Methodsmentioning

confidence: 99%

Three-dimensional bioprinting in ophthalmic care

Al-Atawi

2023

Int J Ophthalmol

View full text Add to dashboard Cite

Three-dimensional (3D) bioprinting is widely used in ophthalmic clinic, including in diagnosis, surgery, prosthetics, medications, drug development and delivery, and medical education. Articles published in 2011–2022 into bioinks, printing technologies, and bioprinting applications in ophthalmology were reviewed and the strengths and limitations of bioprinting in ophthalmology highlighted. The review highlighted the trade-offs of printing technologies and bioinks in respect to, among others, material type cost, throughput, gelation technique, cell density, cell viability, resolution, and printing speed. There is already widespread ophthalmological application of bioprinting outside clinical settings, including in educational modelling, retinal imaging/visualization techniques and drug design/testing. In clinical settings, bioprinting has already found application in pre-operatory planning. Even so, the findings showed that even with its immense promise, actual translation to clinical applications remains distant, but relatively closer for the corneal (except stromal) tissues, epithelium, endothelium, and conjunctiva, than it was for the retina. This review similarly reflected on the critical on the technical, practical, ethical, and cost barrier to rapid progress of bioprinting in ophthalmology, including accessibility to the most sophisticated bioprinting technologies, choice, and suitability of bioinks, tissue viability and storage conditions. The extant research is encouraging, but more work is clearly required for the push towards clinical translation of research.

show abstract

“…In addition, it has been extensively shown that LIFT is one of the bioprinting techniques with the highest cell viability, even better than the widely applied extrusion or inkjet printing methods. It guarantees in general the DNA integrity after the transfer process, despite the fact that the cells could suffer some mechanical stresses due to the dynamics of the liquid transfer process, has it has been published in a very recent work even for cancer cell lines [ 63 ] . However, the fact that direct laser irradiation and/or heating of the media containing the cells could affect cell functionality in any extent beyond DNA integrity has been a constant concern in this field, and a number of approaches envisaged the 78 problem of avoiding direct laser irradiation of the cells.…”

Section: Lasers In Ctcs Researchmentioning

confidence: 99%

Laser transfer for circulating tumor cell isolation in liquid biopsy

Molpeceres¹,

Ramos-Medina²,

Márquez³

et al. 2024

IJB

View full text Add to dashboard Cite

Cancer research has found in the recent years a formidable ally in liquid biopsy, a noninvasive technique that allows the study of circulating tumor cells (CTCs) and biomolecules involved in the dynamics of cancer spread like cell-free nucleid acids or tumor-derived extracellular vesicles. However, single-cell isolation of CTCs with high viability for further genetic, phenotypic, and morphological characterization remains a challenge. We present a new approach for single CTC isolation in enriched blood samples using a liquid laser transfer (LLT) process, adapted from standard laser direct write techniques. In order to completely preserve the cells from direct laser irradiation, we used an ultraviolet laser to produce a blister-actuated laser-induced forward transfer process (BA-LIFT). Using a plasma-treated polyimide layer for blister generation, we completely shield the sample from the incident laser beam. The optical transparency of the polyimide allows direct cell targeting using a simplified optical setup, in which the laser irradiation module, standard imaging, and fluorescence imaging share a common optical path. Peripheral blood mononuclear cells (PBMCs) were identified by fluorescent markers, while target cancer cells remained unstained. As a proof of concept, we were able to isolate single MDA-MB-231 cancer cells using this negative selection process. Unstained target cells were isolated and culture while their DNA was sent for single-cell sequencing (SCS). Our approach appears to be an effective approach to isolate single CTCs, preserving cell characteristics in terms of cell viability and potential for further SCS.

show abstract

Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study

Cited by 16 publications

References 70 publications

Laser Bioprinting with Cell Spheroids: Accurate and Gentle

Laser Bioprinting with Cell Spheroids: Accurate and Gentle

Three-dimensional bioprinting in ophthalmic care

Laser transfer for circulating tumor cell isolation in liquid biopsy

Contact Info

Product

Resources

About