Laser Capture Microdissection in Dentistry

Abstract

Laser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue specimens in dental research. For example, the real-time polymerase-chain reaction (PCR) can be performed from the formaldehyde-fixed, paraffin-embedded, and immunostained sections. Thus, the advance of immuno-LCM method allows us to improve the validity of molecular biological analysis and to get more accurate diagnosis in pathological field in contrast to conventional LCM. This paper is focused on the presentation and discussion of the existing literature that covers the fields of RNA analysis following LCM in dentistry.

A novel whole tooth‐in‐jaw‐bone culture of rat molars: Morphological, immunohistochemical, and laser capture microdissection analysis

“…Laser capture microdissection (LCM) allows microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis (Chokechanachaisakul et al,2010b; Emmert‐Buck et al,1996; Murray,2007). Recently, we have reported a method for immune‐LCM in the formaldehyde‐fixated paraffin embedded (FFPE) tissue sections (Kaneko et al,2009) or nonstained FFPE tissue sections that had been demineralized (Kaneko et al,2010, 2011).…”

Exploring the potential of laser capture microdissection technology in integrated oral biosciences

“…Various modifications of this basic working have been developed. The PixCell system (Arcturus, MDS Analytical Technology, Sunnyvale, CA, USA) uses a dual laser system: the ultraviolet (UV) laser to incise and infrared (IR) laser to gather cells (Chokechanachaisakul et al , ). The PixCell LCM system is the most preferred LCM system in use (Chokechanachaisakul et al , ).…”

“…The PixCell system (Arcturus, MDS Analytical Technology, Sunnyvale, CA, USA) uses a dual laser system: the ultraviolet (UV) laser to incise and infrared (IR) laser to gather cells (Chokechanachaisakul et al , ). The PixCell LCM system is the most preferred LCM system in use (Chokechanachaisakul et al , ). Zeiss's PALM system (a unit of Carl Zeiss Micro Imaging, Jana, Germany) uses a UV laser to incise the tissues via inverted microscope and gather cells by photonic pressure (Chokechanachaisakul et al , ), diagrammatically represented in Figure b.…”

“…The PixCell LCM system is the most preferred LCM system in use (Chokechanachaisakul et al , ). Zeiss's PALM system (a unit of Carl Zeiss Micro Imaging, Jana, Germany) uses a UV laser to incise the tissues via inverted microscope and gather cells by photonic pressure (Chokechanachaisakul et al , ), diagrammatically represented in Figure b. Leica AS LMD system (Mannheim, Germany) uses a UV laser to incise, and then the dismembered cells fall into a collecting tube by gravity (Chokechanachaisakul et al , ), diagrammatically represented in Figure c.…”

“…With the emergence of high‐throughput methods such as DNA microarrays, differential display–comparative genomic hybridization (DD‐CGH), sequential elongation processes, and proteomics, over the last decade, molecular biology has refined the basic understanding of these biological processes and broadened our approach in dealing with the disease. The process of acquiring the pertinent cell or tissue is, however, shrouded by an assorted background of stromal and inflammatory cells at the tissue level, thus raising questions about the precision of these tools (Curran et al , ; Domazet et al , ; Chokechanachaisakul et al , ). The molecular understanding of pathologic processes in the oral cavity is further compounded as the surgical specimens provided are small and often contain an admixture of various cells.…”