Laser Capture Microdissection: Molecular Analysis of Tissue

Abstract

tion and the resulting contaminating , debris are avoided. In addition, only Laser Capture Microdissection: sion the targeted of transfer cells that are affected, can approach with a 1 preci-pm. Molecular Analysis of Tissuen u s , fullv accessible the remaining for further tissue capture, on the allowing slide is coA~arative molecular analisis of adiac& cells: The exact morphology bf the pr&ured Robert F. Bonner, Michael Emmert-Buck, Kristina Cole, Thomas cells is retained andheldonthe transferfilm, Pohida, Rodrigo Chuaqui, Seth Goldstein, Lance A. Liotta so transfer images constitute a diagnostic record of the cells undergoing molecular analysis. In contrast, with manual microdissection, cells may be pulverized or lost (5).A s the list of expressed human genes ex-tion of the cells of interest. A second cat-With LCM, the procurement ofspecific cells pan&, a major scientific and medical chal-egory involves a tool under manual guidance from a complex tissue section is reduced to a lenge is to underused to mechanically separate cells of inter-routine method amenable to widespread re-TE 1 stand the molecular est from the histologic section. The tool can search and clinical diagnostic use.events that drive be a pipette, pointed probe, fine needle, or The thermoplastic polymer film contains normal tissue morphogenesis and the pro-blade (3) either hand-held or connected to a special infrared (IR) absorbing dyes to allow gression ofpathologic lesions in actual tissue. micromanipulator arm (5). Boehm et al. (6) use with near-IR gallium arsenide laser di-With the advent of polymerase chain reac-placed the tissue section on an ultrathin film odes, which are easily and economically intion (PCR) and the development of high-and then used an ultraviolet laser to cut out tegrated into normal microscopes. The polythroughput, automated microhybridization the tissue of interest. mer and its substrate do not absorb the visarrays and mutation screening methods,

Impacts of excision repair cross-complementing gene 1 (ERCC1), dihydropyrimidine dehydrogenase, and epidermal growth factor receptor on the outcomes of patients with advanced gastric cancer

“…Before microdissection, sections were stained with nuclear fast red (American MasterTech Scientific, Lodi, CA, USA). The sections of interest were selectively isolated by laser-captured microdissection (PALM Microsystem, Leica, Wetzlar, Germany), according to standard procedures (Bonner et al, 1997). The dissected particles of tissue were transferred to a reaction tube containing 400 ml of RNA lysis buffer.…”

Clonal relationships between epidermotropic metastatic melanomas and their primary lesions: a loss of heterozygosity and X-chromosome inactivation-based analysis

“…Laser-assisted microdissection of primary and metastatic melanoma was performed ( Figure 2) on unstained sections using a PixCell II Laser Capture Microdissection (LCM) system (Arcturus Engineering, Mountain View, CA, USA) as described previously. [23][24][25][26] Approximately 400-1000 cells of primary and metastatic melanoma were microdissected from the 5-mm histologic sections. Normal tissue from each case was microdissected as a control.…”

Single-Cell Gene Expression Analysis: Implications for Neurodegenerative and Neuropsychiatric Disorders

“…Two widely used microdissection methodologies are laser capture microdissection (LCM) and microaspiration. LCM employs a high-energy laser source that separates desired cells from the rest of a tissue section and facilitates transfer of the identified cells to a microfuge tube for genetic analysis (35,36). Single cells as well as hundreds of cells can be acquired via LCM in a relatively short time.…”