Laser Correlation Spectrometer for Assessing the Size and Dynamics of Changes in the Size of Structures in Biological Fluids

Velichko, Elena; Nepomnyashchaya, Elina; Соколов, А. В.; Kudryashova, Tatyana

doi:10.1134/s0030400x20070255

Cited by 11 publications

(2 citation statements)

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“…We used an original modification of the classical approach of dynamic light scattering measurements by laser correlation spectroscopy, an approbation and detailed description of which were presented in [ 56 , 57 , 58 ]. Our main modification consists of an original scheme shown in Figure 1 and the data processing algorithm adopted for the investigation of polydisperse solutions.…”

Section: Methodsmentioning

confidence: 99%

Aggregation Properties of Albumin in Interacting with Magnetic Fluids

Velichko

Nepomnyashchaya

Baranov

et al. 2021

IJMS

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In this study, interactions of Fe3O4 magnetic nanoparticles with serum albumin biomolecules in aqueous solutions were considered. The studies were conducted with the laser correlation spectroscopy and optical analysis of dehydrated films. It was shown that the addition of magnetite to an albumin solution at low concentrations of up to 10−6 g/L led to the formation of aggregates with sizes of up to 300 nm in the liquid phase and an increase in the number of spiral structures in the dehydrated films, which indicated an increase in their stability. With a further increase in the magnetite concentration in the solution (from 10−4 g/L), the magnetic particles stuck together and to albumin, thus forming aggregates with sizes larger than 1000 nm. At the same time, the formation of morphological structures in molecular films was disturbed, and a characteristic decrease in their stability occurred. Most stable films were formed at low concentrations of magnetic nanoparticles (less than 10−4 g/L) when small albumin–magnetic nanoparticle aggregates were formed. These results are important for characterizing the interaction processes of biomolecules with magnetic nanoparticles and can be useful for predicting the stability of biomolecular films with the inclusion of magnetite particles.

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Section: Methodsmentioning

confidence: 99%

Aggregation Properties of Albumin in Interacting with Magnetic Fluids

Velichko

Nepomnyashchaya

Baranov

et al. 2021

IJMS

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“…В идеале лабораторный метод диагностики АИГ должен позволять детектировать не один тип биохимических маркеров, как это происходит при применении большинства лабораторных тестов, а весь их интегральный спектр. Таким методом может явиться монохромный анализатор наночастиц (МАН), который является усовершенствованной модификацией метода лазерно-корреляционной спектроскопии (ЛКС) [28], что обусловливает их широкое применение в приборах различных фирм для медицинских и биологических исследований [29]. По сравнению с другими методами медико-биологических исследований, метод МАН обладает рядом существенных преимуществ: широким диапазоном исследуемых фракций, возможностью одновременного анализа частиц разных гидродинамиических диаметров (структурнофункциональный анализ молекулярных ингредиентов биологических жидкостей), учётом характера межмолекулярных взаимодействий отдельных ингредиентов, достаточностью минимального количества исследуемого биоматериала, простой процедурой подготовки образцов к анализу, высокой скоростью измерений изучаемого образца и получения качественной и количественной информации [30].…”

Section: резюмеunclassified

Algorithm of rapid diagnosis of chronic autoimmune hepatitis by saliva by the method of monochrome analysis of nanoparticles

Choi¹

2022

jour

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Сhronic autoimmune hepatitis is an urgent problem of modern medicine due to the widespread prevalence among all autoimmune diseases (more than 25%) and the diffi culty of its diagnosis due to the lack of reliable serological tests for its detection. The purpose of this work was to study the possibility of the method of monochrome analysis of nanoparticles in noninvasive detection of AIG and to develop an algorithm for rapid diagnosis of chronic autoimmune hepatitis by saliva.The goal was solved by performing a number of tasks: the development of a diagnostic algorithm for monochrome analysis of nanoparticles to determine the severity and pathophysiological orientation of homeostatic shifts in patients with a verifi ed diagnosis of chronic autoimmune hepatitis using samples of oropharyngeal fl ushes (saliva), as well as the calculation of diagnostic specifi city and sensitivity of the test. Materials and methods:The studies were conducted at the Center for European and Oriental Medicine from 2019 to 2022, and 14 patients with a verifi ed diagnosis of autoimmune hepatitis were examined. It was found that the most typical saliva spectra of patients with autoimmune liver damage were characterized by three-modality and predominance of light scattering on particles of super-large diameter (over 1000 nm) and a signifi cant increase in the contribution to light scattering of nanoparticles of small and medium hydrodynamic size, which was statistically signifi cant (p<0.001) when conducting a comparative analysis with the saliva spectra of practically healthy individuals and patients with general somatic liver diseases of non-autoimmune genesis. The indicators of diagnostic sensitivity for autoimmune hepatitis were 95%, diagnostic specifi city -93%.Conclusions: the results of the conducted scientifi c research were the developed algorithm for the use of laser spectroscopy of saliva for noninvasive rapid diagnosis of chronic autoimmune hepatitis, when with a timely diagnosis, therapeutic measures will be maximally eff ective and aimed at preventing complications and systemic manifestations of the disease, and the calculation of diagnostic sensitivity and specifi city of the method of monochrome analysis of nanoparticles.

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