Laser desorption ionization mass spectrometry of protein tryptic digests on nanostructured silicon plates

Dupré, Mathieu; Coffinier, Yannick; Boukherroub, Rabah; Cantel, Sonia; Martinez, Jean; Enjalbal, Christine

doi:10.1016/j.jprot.2011.12.039

Cited by 34 publications

(42 citation statements)

References 48 publications

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“…Indeed, nanostructured silicon substrates, [8][9][10][11][12][13][14][15] nanostructured diamond, 16,17 germanium nanodots, 18 gold 19-21 and silver nanoparticles, 22,23 carbon nanotubes, 24,25 platinum nanoflowers, 26 zinc oxide (ZnO) nanowires, 27 and porous alumina 28 have successfully been used as highly efficient matrices in SALDI-MS. Indeed, nanostructured silicon substrates, [8][9][10][11][12][13][14][15] nanostructured diamond, 16,17 germanium nanodots, 18 gold 19-21 and silver nanoparticles, 22,23 carbon nanotubes, 24,25 platinum nanoflowers, 26 zinc oxide (ZnO) nanowires, 27 and porous alumina 28 have successfully been used as highly efficient matrices in SALDI-MS.…”

Section: Introductionmentioning

confidence: 99%

Surface-assisted laser desorption–ionization mass spectrometry on titanium dioxide (TiO2) nanotube layers

Piret

Kim

Drobecq

et al. 2012

Analyst

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The paper reports on the use of a titanium oxide (TiO(2)) nanotube layer as a sensitive substrate for surface-assisted laser desorption-ionization mass spectrometry (SALDI-MS) of peptides and small molecules. The nanotube layers were prepared by electrochemical anodization of titanium foil. The optimized TiO(2) nanotubes morphology coupled to a controlled surface chemistry allowed desorption-ionization (D/I) of a peptide mixture (Mix1) with a detection limit of 10 femtomoles for the neurotensin peptide. The performance of the TiO(2) nanotubes for the D/I of small molecules was also tested for the detection of sutent, a small tyrosine kinase inhibitor, and verapamil. A detection limit of 50 fmol was obtained for these molecules, as compared to 500 fmol using classical matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS). Both amorphous and anatase TiO(2) layers displayed a comparable performance for D/I of analyte molecules. In a control experiment, we have performed D/I of analyte molecules on a flat TiO(2) layer. The absence of signal emphasizes the role of the nanostructured substrate in the D/I process.

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Section: Introductionmentioning

confidence: 99%

Surface-assisted laser desorption–ionization mass spectrometry on titanium dioxide (TiO2) nanotube layers

Piret

Kim

Drobecq

et al. 2012

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“…It has shown that real biological samples (BSA digest, carnitine metabolites) can also be analyzed on those surfaces as a proof of concept. Dupre et al (2012) has applied a similar approach for high throughput identification of tryptic digests, which is the starting point of MS-based protein identification studies. They showed that tryptic peptides from cytochrome C, beta casein, BSA, and fibrinogen can be analyzed with femtomolar sensitivity and improved sequence coverage can be obtained.…”

Section: Nanostructured Surfacesmentioning

confidence: 99%

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Kobeissy

Gülbakan

Alawieh

et al. 2014

OMICS: A Journal of Integrative Biology

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The post-genomics era has brought about new Omics biotechnologies, such as proteomics and metabolomics, as well as their novel applications to personal genomics and the quantified self. These advances are now also catalyzing other and newer post-genomics innovations, leading to convergences between Omics and nanotechnology. In this work, we systematically contextualize and exemplify an emerging strand of post-genomics life sciences, namely, nanoproteomics and its applications in health and integrative biological systems. Nanotechnology has been utilized as a complementary component to revolutionize proteomics through different kinds of nanotechnology applications, including nanoporous structures, functionalized nanoparticles, quantum dots, and polymeric nanostructures. Those applications, though still in their infancy, have led to several highly sensitive diagnostics and new methods of drug delivery and targeted therapy for clinical use. The present article differs from previous analyses of nanoproteomics in that it offers an in-depth and comparative evaluation of the attendant biotechnology portfolio and their applications as seen through the lens of post-genomics life sciences and biomedicine. These include: (1) immunosensors for inflammatory, pathogenic, and autoimmune markers for infectious and autoimmune diseases, (2) amplified immunoassays for detection of cancer biomarkers, and (3) methods for targeted therapy and automatically adjusted drug delivery such as in experimental stroke and brain injury studies. As nanoproteomics becomes available both to the clinician at the bedside and the citizens who are increasingly interested in access to novel post-genomics diagnostics through initiatives such as the quantified self, we anticipate further breakthroughs in personalized and targeted medicine.

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“…Other efficient mass spectrometric based approaches have been reported for biofluids analysis such as time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS), desorption electrospray ionization (DESI)‐MS or direct analysis in real time (DART), to name but a few . Nevertheless, in contrast to such mainstream analytical methods, we have already investigated, as have others, inert substrates to replace conventional organic matrices to promote ion formation in so‐called surface‐assisted laser desorption/ionization mass spectrometry (SALDI‐MS) . Substitution of an organic matrix by a UV‐absorbing nanomaterial represents an attractive alternative to matrix‐assisted laser desorption/ionization (MALDI)‐MS.…”

Section: Introductionmentioning

confidence: 99%

Fast and facile preparation of nanostructured silicon surfaces for laser desorption/ionization mass spectrometry of small compounds

Hamdi

Enjalbal

Drobecq

et al. 2018

Rapid Comm Mass Spectrometry

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Laser desorption ionization mass spectrometry of protein tryptic digests on nanostructured silicon plates

Cited by 34 publications

References 48 publications

Surface-assisted laser desorption–ionization mass spectrometry on titanium dioxide (TiO2) nanotube layers

Surface-assisted laser desorption–ionization mass spectrometry on titanium dioxide (TiO2) nanotube layers

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Fast and facile preparation of nanostructured silicon surfaces for laser desorption/ionization mass spectrometry of small compounds

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