Laser Diagnostics of the Mesoscale Heterogeneity of Aqueous Solutions of Polar Organic Compounds

Bunkin, N. F.; Lyakhov, G. A.; Shkirin, A. V.; Krivokhizha, S. V.; Aleksey, Afonin; Kobelev, A. V.; Penkov, Nikita V.; Фесенко, Е. Е.

doi:10.3103/s1541308x18010041

Cited by 16 publications

(13 citation statements)

References 31 publications

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“…Because of the elimination of large particles and a decrease in the total scattering intensity by 116 times, individual molecules (12 nm) become visible. Furthermore, there can be seen a peak at ∼150 nm, which is characteristic for pure ethanol mesodroplets in an EWM, 32,33 and a peak at d = 12 nm, corresponding to monomeric IgG molecules. To trace the behavior of monomer and aggregate peaks, we applied the shaking procedure (described in Section 5.1) to the filtered IgG solution (Figure 11b); the resulting distribution is shown in Figure 11c.…”

Section: Resultsmentioning

confidence: 96%

“…The height of the profile Δh makes it possible to assign the observed inhomogeneities to mesodroplets enriched with ethanol, see our recent studies. 32,33 However, we cannot exclude that in that case, we deal with artifacts associated with interference noise. 34 Thus, we conclude that in this case, the scatterers are liquid mesodroplets, whose volume number density is not changed in the process of shaking.…”

Section: Resultsmentioning

confidence: 99%

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Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water–Ethanol Mixtures

et al. 2020

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Structural characterization by three complementary methods of laser diagnostics (dynamic light scattering, laser phase microscopy, and laser polarimetric scatterometry) has established that shaking of immunoglobulin G (IgG) dispersions in water and ethanol–water mixtures (36.7 vol %) results in two effects. First, it intensifies the aggregation of IgG macromolecules. Second, it generates bubbles with a size range that is different in each solvent. The aggregation is enhanced in ethanol–water mixtures because of IgG denaturation. IgG aggregates have a size of ∼300 nm in water and ∼900 nm in ethanol–water mixtures. The flotation of IgG is much more efficient in water. This can be explained by a better adsorption of IgG particles (molecules and aggregates) on bubbles in water as compared to ethanol–water mixtures. Bulk nanobubbles and their association with IgG aggregates were visualized by laser phase microscopy in water but were not detected in ethanol–water mixtures. Therefore, the nanobubble flotation mechanism for IgG aggregates acting in water is not feasible for ethanol–water mixtures.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water–Ethanol Mixtures

et al. 2020

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Section: Experimental Section and Computational Methodsmentioning

confidence: 99%

“…White light microphotographs of the powders were made on a substrate in an air atmosphere. A laser phase microscope, which displays a 2D distribution of the OPD in a plane transverse to the laser beam, was used to measure the refractive index of dispersed submicron particles …”

Section: Experimental Section and Computational Methodsmentioning

confidence: 99%

“…A laser phase microscope, which displays a 2D distribution of the OPD in a plane transverse to the laser beam, was used to measure the refractive index of dispersed submicron particles. 67 The light scattering matrices of the suspensions were measured using a laser polarimeter in a scattering angle range from 20 to 150°. 68 A single-mode He−Ne laser with a wavelength of 0.63 μm and a power of 7 mW was used as a radiation source.…”

Section: Study Implications and Outlookmentioning

confidence: 99%

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Identification of Organic Matter Dispersions Based on Light Scattering Matrices Focusing on Soil Organic Matter Management

et al. 2020

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The origin of organic matter, its spread, scattering, and functioning are influenced by the physical structure of liquid or dispersed media of organic matter. Refractive indices of fodder yeast grown on paraffin oil (paprin) and natural gas (gaprin) as well as Lycoperdon spore and organelles were measured by laser phase microscopy. The scattering matrices of aqueous suspensions of paprin, gaprin, and Lycoperdon spores were measured using a laser polarimeter with the scattering angle ranging from 20 to 150°. The experimentally measured scattering matrices have been approximated by the weighted sum of theoretically calculated scattering matrices using the T-matrix code developed by Mishchenko. Most of the particle radii in the filtered fraction of paprin and gaprin were within the range of about 0.05−0.12 μm. Particle radii of the Lycoperdon spore suspension were within the range of 0.4−2.4 μm, which corresponded to both whole spores and their separate organelles. A possibility of identifying a suspension by its scattering matrices was shown for a small difference in the real parts of the refractive index in the example of paprin and gaprin. The measurements of the light scattering matrix showed that for a small size parameter of about 1, the identification of paprin and gaprin can be based only on a difference in the particle shape. Refractive index difference is manifested for the size parameter values higher than 3. An example of a suspension consisting of micron-sized spores and their submicron organelles shows high sensitivity of the scattering matrix to the composition of the dispersed material. The presented data and models help to extrapolate the results of the light scattering matrix study to a vast spectrum of media of organic matter origin and functioning. This study focused on the Biogeosystem Technique (BGT*) transcendental methodology to manage soil as an arena of biodegradation and organic synthesis. A BGT*-based robotic system for intra-soil pulse continuous−discrete water and matter supply directly into the dispersed−aggregated physical structure of the soil media was developed. The system enables transformation of soil into a stable highly productive organic chemical bioreactor for better controlled nanoparticle biomolecular interactions and adsorption by biological and mineral media. The scattering matrix measurement unit is supposed to be used in the robotic system as a diagnostic tool for the dispersion composition of soil organic components.

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Synthesis and characterization of novel poly(ionic liquid)s and their viscosity-increasing effect

Zang

Chang

Zheng

et al. 2020

Journal of Molecular Liquids

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Laser Diagnostics of the Mesoscale Heterogeneity of Aqueous Solutions of Polar Organic Compounds

Cited by 16 publications

References 31 publications

Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water–Ethanol Mixtures

Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water–Ethanol Mixtures

Identification of Organic Matter Dispersions Based on Light Scattering Matrices Focusing on Soil Organic Matter Management

Synthesis and characterization of novel poly(ionic liquid)s and their viscosity-increasing effect

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