Laser diode thermal desorption–positive mode atmospheric pressure chemical ionization tandem mass spectrometry for the ultra-fast quantification of a pharmaceutical compound in human plasma

Heudi, Olivier; Barteau, Samuel; Picard, Pierre; Tremblay, Patrice; Picard, Franck; Kretz, Olivier

doi:10.1016/j.jpba.2010.11.025

Cited by 24 publications

(17 citation statements)

References 26 publications

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“…Analysis times were reduced from minutes by current LC-MS/MS approaches to seconds, and more than 1000 LC-MS/MS analyses may be performed in less than 1.5 h [24]. Laser diode thermal desorption has been used for the analysis of drugs and metabolites such as SMAs, BZE and synthetic cannabinoids, in quantitative applications [25]. In addition, accurate mass (high-resolution) MS and MS/MS instruments such as those used by some investigators [26,27] have improved specificity for drug and metabolite analyses when compared with the quadrupole instruments that predominate in laboratories today.…”

Section: Technological Improvementsmentioning

confidence: 99%

Oral Fluid for The Detection of Drugs of Abuse Using Immunoassay and LC–MS/MS

Moore

Crouch²

2013

Bioanalysis

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The utility of oral fluid as a sample matrix for the analysis of drugs has been increasing in popularity over the last few years. This is largely because of collection advantages over other matrices, but also due to the rapid improvements in analytical assays including highly sensitive liquid reagent format enzyme immunoassays and LC-MS/MS. This review will highlight improvements in assay formats, sensitivity, laboratory equipment and sample processing using low sample volumes to expand drug test profiles.

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Section: Technological Improvementsmentioning

confidence: 99%

Oral Fluid for The Detection of Drugs of Abuse Using Immunoassay and LC–MS/MS

Moore

Crouch²

2013

Bioanalysis

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“…Analytical runs are thus reduced by about twenty to fifty-fold compared with conventional LC-MS/MS methods. LDTD has been used in a number of analytical applications, for instance, in the determination of pharmaceutical residues in wastewater [19], dairy milk [20] and human plasma [21], steroid hormones [22] and pesticides [23] in wastewater, and cyanobacterial toxins in surface water [24]. However, since LDTD acquisition runs are about 9-s long, this usually does not allow for more than 6-8 transitions to be properly acquired at once when interfaced to a triple quadrupole (QqQ) mass spectrometer, due to dwell time limitations [23].…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of poly- and perfluoroalkyl compounds in water matrices using high resolution mass spectrometry: Optimization for a laser diode thermal desorption method

Munoz

Duy

Budzinski

et al. 2015

Analytica Chimica Acta

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“…The LDTD-APCI ion source has been applied for qualitative and quantitative analyses of different compounds in environmental science [13][14][15][16] as well as in the food area [17][18][19]. More recently, the use of the LDTD-APCI ion source has gained importance in bioanalysis and several authors have demonstrated its benefits in the field of quantitative analysis of small molecules in various biological fluids [20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Laser diode thermal desorption atmospheric pressure chemical ionization tandem mass spectrometry applied for the ultra-fast quantitative analysis of BKM120 in human plasma

et al. 2014

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A sensitive and ultra-fast method utilizing the laser diode thermal desorption ion source using atmospheric pressure chemical ionization coupled to tandem mass spectrometry (LDTD-APCI-MS/MS) was developed for the quantitative analysis of BKM120, an investigational anticancer drug in human plasma. Samples originating from protein precipitation (PP) followed by salting-out assisted liquid-liquid extraction (SALLE) were spotted onto the LazWell™ plate prior to their thermal desorption and detection by tandem mass spectrometry in positive mode. The validated method described in this paper presents a high absolute extraction recovery (>90 %) for BKM120 and its internal standard (ISTD) [D8]BKM120, with precision and accuracy meeting the acceptance criteria. Standard curves were linear over the range of 5.00 to 2000 ng mL(-1) with a coefficient of determination (R (2)) >0.995. The method specificity was demonstrated in six different batches of human plasma. Intra- and inter-run precision as well as accuracy within ±20 % at the lower limit of quantification (LLOQ) and ±15 % (other levels) were achieved during a three-run validation for quality control (QC) samples. The post-preparative stability on the LazWell™ plate at room temperature was 72 h and a 200-fold dilution of spiked samples was demonstrated. The method was applied successfully to three clinical studies (n = 847) and cross-checked with the validated LC-ESI-MS/MS reference method. The sample analysis run time was 10 s as compared to 4.5 min for the current validated LC-ESI-MS/MS method. The resultant data were in agreement with the results obtained using the validated reference LC-ESI-MS/MS assay and the same pharmacokinetic (PK) parameters were calculated for both analytical assays. This work demonstrates that LDTD-APCI-MS/MS is a reliable method for the ultra-fast quantitative analysis of BKM120 which can be used to speed-up and support its bioanalysis in the frame of the clinical trials.

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Laser diode thermal desorption–positive mode atmospheric pressure chemical ionization tandem mass spectrometry for the ultra-fast quantification of a pharmaceutical compound in human plasma

Cited by 24 publications

References 26 publications

Oral Fluid for The Detection of Drugs of Abuse Using Immunoassay and LC–MS/MS

Oral Fluid for The Detection of Drugs of Abuse Using Immunoassay and LC–MS/MS

Quantitative analysis of poly- and perfluoroalkyl compounds in water matrices using high resolution mass spectrometry: Optimization for a laser diode thermal desorption method

Laser diode thermal desorption atmospheric pressure chemical ionization tandem mass spectrometry applied for the ultra-fast quantitative analysis of BKM120 in human plasma

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