Laser Effects on Blood, Blood Vessels and Blood Vessel Tumors

Goldman, Leon

doi:10.1007/978-3-642-85797-3_13

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1994

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“…This implies either that Ca2+ extrusion was incompletely blocked or that other mechanisms, such as mitochondria (44) or TG-insensitive SR Ca2+-ATPase (8,11), buffer the rise in [Ca2ecyt. In contrast, application of 10 mM caffeine to fura-2-loaded A7r5 cells failed to elicit a significant rise in [Ca2W1,t in either the Ca2+-confining medium or PSS ( A caffeine-induced decrease in [Ca2+]kt in A7r5 cells has been reported by others (37). Also, the response to caffeine (and ryanodine) may be down-regulated in cultured VSM cells (45,46 (Fig.…”

Section: Methodsmentioning

confidence: 65%

Functionally and spatially distinct Ca2+ stores are revealed in cultured vascular smooth muscle cells.

Tribe

Borin²,

Blaustein³

1994

Proc. Natl. Acad. Sci. U.S.A.

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sequester Ca2+ was inhibited. This suggests that the caffeinesensitive store may have a thapsigargin-insensitive Ca2+-sequestration mechanism. To complement these fura-2 experiments, chlortetracycline was used to visualize the Ca2+ stores directly. The latter studies revealed spatial differences in the location of the thapsigargin-sensitive and caffeine-sensitive Ca2+ stores (measured as thapsigargin-sensitive and caffeinesensitive chlortetracyclin fluorescence). Thus, these two types of stores appear to be both functionally and spatially distinct.

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Section: Methodsmentioning

confidence: 65%