Laser excited fluorescence spectroscopic studies of cellular macromolecular damage from chemical carcinogens

Lu, Peiqi

doi:10.31274/rtd-180813-11048

Cited by 1 publication

(1 citation statement)

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“…For example, it was shown that the major stable DNA adduct from the liver of fish exposed to high dosage levels of BP is derived from syn-BPDE (20), in agreement with previous work (23). At low dosage levels, the major adduct was found to originate from (+)-anti-BPDE (23,24). FLNS was used to prove (22) that the major human globin adduct from BPDE is a carboxylic ester and that this adduct is of the interior type (i.e., undetectable in the intact protein by monoclonal antibody approaches).…”

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confidence: 89%

Comparative laser spectroscopic study of DNA and polynucleotide adducts from the (+)-anti-diol epoxide of benzo[a]pyrene

Lu¹,

Jeong²,

Jankowiak³

et al. 1991

Chem. Res. Toxicol.

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A recently developed methodology [Jankowiak, R., Lu, P., Small, G. J., and Geacintov, N. E. (1990) Chem. Res. Toxicol. 3, 39-46], which combines fluorescence line narrowing spectroscopy at 4.2 K with non-line-narrowed (S2----S0 laser excitation) fluorescence spectroscopy at 77 K and fluorescence quenching, is used to characterize adducts formed from (+)-anti-BPDE and the alternating copolymers poly(dG-dC).poly(dG-dC) and poly(dA-dT).poly(dA-dT), the nonalternating poly (dG).poly(dC), single-strand poly(dG), and the oligonucleotide d(ATATGTATA). Detailed comparisons of the fluorescence spectra and quenching (with acrylamide) of the properties of the adducts with those of (+)-anti-BPDE-DNA adducts are made. Fluorescence spectra of the trans and cis isomers of the adduct formed from guanosine monophosphate and the adducts of d(ATATGTATA) are used to assign the stereochemistry of the two major DNA adducts as trans-N2-dG moieties which occupy two different DNA sites. Evidence for the existence of minor cis-type guanine adducts is provided. Finally, a fourth type of DNA adduct (minor) is identified and assigned as trans-N6-dA.

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