Laser-Excited Time-Resolved Shpol'skii Spectroscopy for the Direct Analysis of Dibenzopyrene Isomers in Liquid Chromatography Fractions

Yu, Shenjiang; Campiglia, Andrés D.

doi:10.1366/0003702042641416

Cited by 21 publications

(18 citation statements)

References 15 publications

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“…In previous studies, Campiglia and co-workers developed methodology for the analysis of DBalP, DBaeP, DBaiP, DBelP, DBahP and N23aP in environmental extracts on the bases of spectral and lifetime data. 7,20,21,32–34 Fluorescence spectra and lifetimes were recorded from n -octane solutions at liquid nitrogen (77 K) and liquid helium (4.2 K) temperatures. The spectral resolution achieved under Shpol’skii conditions provided vibrational fingerprints for the determination of the six isomers with or without previous RPLC separation.…”

Section: Resultsmentioning

confidence: 99%

“…PAH detection was carried out in the UV detection mode at 254 nm, which is commonly used in the literature for the determination of PAHs via RPLC/UV. 17,19,20 The RPLC/UV chromatogram obtained for the 23 MM 302 PAH isomers is shown in Fig. S3.…”

Section: Resultsmentioning

confidence: 99%

“…Gas chromatography/mass spectrometry (GC/MS) 6, 9–11, 13, 16–18 and reverse-phase liquid chromatography with ultraviolet absorption (RPLC/UV) 17–20 or fluorescence detection (RPLC/FL) 7,8,12,14 are popular techniques for the analysis of PAHs in combustion-related samples. Similar chromatographic behaviors make the analysis of MM 302 PAHs a particularly challenging task.…”

Section: Introductionmentioning

confidence: 99%

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Determination of polycyclic aromatic hydrocarbons with molecular mass 302 in standard reference material 1597a by reversed-phase liquid chromatography and stop-flow fluorescence detection

et al. 2018

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The identification of isomeric polycyclic aromatic hydrocarbons (PAHs) in complex samples via reversed-phase liquid chromatography (RPLC) with fluorescence detection (FL) is normally based on matching the chromatographic retention times of suspected peaks of interest with reference standards. Since no spectral information is obtained during the chromatographic run, the accurate identification of co-eluting PAHs with similar chromatographic behaviors requires confirmation with additional chromatographic methods. This is particularly true for the analysis of PAH isomers with the relative molecular mass (MM, g/mol) 302. The work presented here explores the information content of room-temperature fluorescence spectra for the analysis of PAHs with MM 302 in the Standard Reference Material (SRM) 1597a. Fluorescence spectra were recorded under stop-flow conditions with the aid of a commercial HPLC system. Of the 21 MM 302 PAHs known to be present in the SRM 1597a, 20 were tentatively identified based on retention times and the presence of 18 was confirmed based on excitation and emission spectral profiles.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of polycyclic aromatic hydrocarbons with molecular mass 302 in standard reference material 1597a by reversed-phase liquid chromatography and stop-flow fluorescence detection

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“…These include liquid-liquid extraction (LLE) [24, 34, 37], solid-phase extraction (SPE) [24, 34, 37], evaporation (EVAP) [1, 33] and SPNE [25]. This article compares the analytical performance of EVAP and SPNE for the LETRSS analysis of the five targeted isomers.…”

Section: Resultsmentioning

confidence: 99%

Determination of high-molecular weight polycyclic aromatic hydrocarbons in high performance liquid chromatography fractions of coal tar standard reference material 1597a via solid-phase nanoextraction and laser-excited time-resolved Shpol'skii spectroscopy

Wilson

Alfarhani

Moore

et al. 2016

Talanta

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This article presents an alternative approach for the analysis of high molecular weight – polycyclic aromatic hydrocarbons (HMW-PAHs) with molecular mass 302 Da in complex environmental samples. This is not a trivial task due to the large number of molecular mass 302 Da isomers with very similar chromatographic elution times and similar, possibly even virtually identical, mass fragmentation patterns. The method presented here is based on 4.2 K laser-excited time-resolved Shpol'skii spectroscopy, a high resolution spectroscopic technique with the appropriate selectivity for the unambiguous determination of PAHs with the same molecular mass. The potential of this approach is demonstrated here with the analysis of a coal tar standard reference material (SRM) 1597a. Liquid chromatography fractions were submitted to the spectroscopic analysis of five targeted isomers, namely dibenzo[a,l]pyrene, dibenzo[a,e]pyrene, dibenzo[a,i]pyrene, naphtho[2,3-a]pyrene and dibenzo[a,h]pyrene. Prior to analyte determination, the liquid chromatographic fractions were pre-concentrated with gold nanoparticles. Complete analysis was possible with microliters of chromatographic fractions and organic solvents. The limits of detection varied from 0.05 (dibenzo[a,l]pyrene) to 0.24 μg L−1 (dibenzo[a,e]pyrene). The excellent analytical figures of merit associated to its non-destructive nature, which provides ample opportunity for further analysis with other instrumental methods, makes this approach an attractive alternative for the determination of PAH isomers in complex environmental samples.

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“…(vi) Other methods of quantification Laser-excited time-resolved Shpol'skii spectroscopy has recently been reported as a method for the unambiguous determination of dibenzo [a,e]pyrene, dibenzo [a,h]pyrene, dibenzo [a,i]pyrene, dibenzo [a,l]pyrene and dibenzo [e,l]pyrene in HPLC fractions (Yu & 40 IARC MONOGRAPHS VOLUME 92 Campiglia, 2004). The use of multidimensional GC, LC, coupled LC-GC and supercritical fluid chromatography have been reported (Sonnefeld et al, 1982;Benner, 1998;Poster et al, 1998;Marriott et al, 2003).…”

Section: (Iv) Liquid Chromatography (Lc)mentioning

confidence: 99%

Integrating Field Analyses with Laboratory Exposures to Assess Ecosystems Health

Hellou¹,

Beach²,

Leonard³

et al. 2012

Polycyclic Aromatic Compounds

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Laser-Excited Time-Resolved Shpol'skii Spectroscopy for the Direct Analysis of Dibenzopyrene Isomers in Liquid Chromatography Fractions

Cited by 21 publications

References 15 publications

Determination of polycyclic aromatic hydrocarbons with molecular mass 302 in standard reference material 1597a by reversed-phase liquid chromatography and stop-flow fluorescence detection

Determination of polycyclic aromatic hydrocarbons with molecular mass 302 in standard reference material 1597a by reversed-phase liquid chromatography and stop-flow fluorescence detection

Determination of high-molecular weight polycyclic aromatic hydrocarbons in high performance liquid chromatography fractions of coal tar standard reference material 1597a via solid-phase nanoextraction and laser-excited time-resolved Shpol'skii spectroscopy

Integrating Field Analyses with Laboratory Exposures to Assess Ecosystems Health

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