Laser Induced Autofluorescence Diagnosis of Bladder Tumors: Dependence on the Excitation Wavelength

Anidjar, Maurice; Ettori, Dominique; Cussenot, Olivier; Méria, P.; Desgrandchamps, François; Cortesse, A.; Teillac, P.; Duc, A. Le; Avrillier, Sigrid

doi:10.1016/s0022-5347(01)65455-7

Cited by 61 publications

(41 citation statements)

References 16 publications

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“…4,8,9,12,19,[23][24][25][26][27][28][29] All methods were performed on the basis of leave-one-out classification, to ensure that no overly positive results were obtained. We investigated all six excitation wavelengths.…”

Section: Classification Of (Pre-)malignant Versus Benign Lesionsmentioning

confidence: 99%

Clinical study for classification of benign, dysplastic, and malignant oral lesions using autofluorescence spectroscopy

et al. 2004

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Abstract. Autofluorescence spectroscopy shows promising results for detection and staging of oral (pre-)malignancies. To improve staging reliability, we develop and compare algorithms for lesion classification. Furthermore, we examine the potential for detecting invisible tissue alterations. Autofluorescence spectra are recorded at six excitation wavelengths from 172 benign, dysplastic, and cancerous lesions and from 97 healthy volunteers. We apply principal components analysis (PCA), artificial neural networks, and red/green intensity ratio's to separate benign from (pre-)malignant lesions, using four normalization techniques. To assess the potential for detecting invisible tissue alterations, we compare PC scores of healthy mucosa and surroundings/contralateral positions of lesions. The spectra show large variations in shape and intensity within each lesion group. Intensities and PC score distributions demonstrate large overlap between benign and (pre-)malignant lesions. The receiver-operator characteristic areas under the curve (ROC-AUCs) for distinguishing cancerous from healthy tissue are excellent (0.90 to 0.97).

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Section: Classification Of (Pre-)malignant Versus Benign Lesionsmentioning

confidence: 99%

Clinical study for classification of benign, dysplastic, and malignant oral lesions using autofluorescence spectroscopy

et al. 2004

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“…Autofluorescence has been used to distinguish adenomatous polyps and normal colon 8 . Other sites for autofluorescence detection are, for example, the urinary bladder and the bronchus 9,10 . Using a 460-nm excitation wavelength, squamous intraepithelial lesions of the portio uteri can be differentiated from normal squamous tissue with an average sensitivity and specificity of 91% and 75.5%, respectively 11 .…”

Section: Introductionmentioning

confidence: 99%

Autofluorescence spectroscopy for the diagnosis of cervical intraepithelial neoplasia

Weingandt

2002

BJOG: An International Journal of Obstetrics and Gynaecology

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Objective To assess the feasibility of autofluorescence spectroscopy in the diagnosis of cervical intraepithelial neoplasia (CIN) using broadband light excitation. Design Feasibility study.Setting Colposcopy clinic of an university hospital.Population Sixty-eight patients at risk for CIN.Methods After excitation with a broadband light between 375 and 440 nm, spectral distribution of native tissue fluorescence (autofluorescence) was acquired from 685 cervical sites for the localisation and differentiation of CIN, and compared with colposcopically directed biopsy and human papillomavirus (HPV) DNA testing. Main outcome measure Detection of CIN.Results The evaluation of spectral measurements revealed significantly lower autofluorescence values for CIN 3 lesions compared with normal tissue ( P < 0.001), and compared with CIN 1 or CIN 2 ( P < 0.002). High grade CIN lesions (CIN 2/3) presented with a significant reduced autofluorescence compared with CIN 1 ( P < 0.002). Patients with a positive HPV DNA testing showed a significantly lower autofluorescence than patients tested negative for HPV DNA ( P < 0.05). Severe inflammation such as chronic cervicitis may lead to false positive results. Conclusions Autofluorescence spectroscopy represents an interesting approach for the detection of cervical neoplasia. Using an excitation wavelength band between 375 and 440 nm, significant differences between normal and precancerous lesions of the cervix can be seen.

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“…Some of literatures mentioned that a pulsed N 2 laser with emission wavelength 337.1 nm was used for photodynamic detection of tumor tissues and mentioned about the using of N2 laser as a source in the photodynamic therapy of brain tumors [9,10].TEA(Transverse Excited Atmospheric) N 2 laser transverse arrangement mode of electrodes is used for the discharge because of its more efficient. An ignition system in the form of spark gap is used to short the Blumlein circuit.…”

Section: Introductionmentioning

confidence: 99%

Study the Effect of the Homemade Nitrogen Laser System in Medical Field

B. Mohammed,

M. Najim,

N. Mohameed

2021

Iraqi Journal of Science

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A high power N 2 laser of the three stage-Blumlein circuit designed, which consist two identical electrodes, four capacitors and three spark gaps which designed discharge geometry with minimum inductance. This system gives a maximum energy 18 mJ with a pulse time 6.65 ns and wave length 337.1 nm. The distance between spark gap terminals has large effect on the output energy. The ideal distance were 11 mm between the terminals for the first spark gap and 4 mm between the terminals for the second spark gap, the distance between the terminals of the spark gaps has a significant effect on the resulting energy, where the energy decreases when the distance between the terminals of the first and second spark gaps exceeds or Less than from the optimum distances. The effect of N 2 laser on the viability of Staphylococcus that isolated from wounds, the samples of bacteria were irradiated with 337.1 nm .N 2 laser using 10 pulses/second repetition rates at 10,15 minutes exposure times. The results showed that the viability of bacteria decreased with increasing the exposure time to N 2 laser.Keywords: Nitrogen laser, Staphylococcus aureus, Bacterial susceptibility. ‫الطبي‬ ‫المجال‬ ‫في‬ ‫الصنع‬ ‫محلية‬ ‫نيتروجين‬ ‫ليزر‬ ‫منظومة‬ ‫تأثير‬ ‫اسة‬ ‫در‬ ‫محمد‬ ‫باسم‬ ‫سيف‬

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Laser Induced Autofluorescence Diagnosis of Bladder Tumors: Dependence on the Excitation Wavelength

Abstract: This spectroscopic study could be particularly useful to design a simplified autofluorescence imaging device for detection of occult urothelial neoplasms.

Cited by 61 publications

References 16 publications

Clinical study for classification of benign, dysplastic, and malignant oral lesions using autofluorescence spectroscopy

Clinical study for classification of benign, dysplastic, and malignant oral lesions using autofluorescence spectroscopy

Autofluorescence spectroscopy for the diagnosis of cervical intraepithelial neoplasia

Study the Effect of the Homemade Nitrogen Laser System in Medical Field

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