Laser-Induced Fluorescence Studies of Hematoporphyrin Derivative (HPD) in Normal and Tumor Tissue of Rat

Ankerst, Jaro; Montán, S.; Svanberg, Katarina; Svanberg, Sune

doi:10.1366/0003702844554738

Cited by 62 publications

(15 citation statements)

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“…The ratio is higher for tumour than for any other of the studied organs. This is because of a decrease of the autofluorescence intensity in the tumour, as has been shown previously (Ankerst et al, 1984;Hung et al, 1991).…”

Section: Materials and Methods Chemicalssupporting

confidence: 62%

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Nilsson

Johansson²,

Svanberg³

et al. 1997

Br J Cancer

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Summary The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.

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Section: Materials and Methods Chemicalssupporting

confidence: 62%

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Nilsson

Johansson²,

Svanberg³

et al. 1997

Br J Cancer

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“…When employing HPD-PDT in liver tumours it is important to consider that normal liver tissue accumulates HPD to a very high degree (Gomer and Dougherty, 1979;Bugelski et al, 1981;Ankerst et al, 1984;Svanberg et al, 1986). For clinical use the dose-dependent transient skin sensitisation and the non-optimal light absorption profile have been considered as other obstacles.…”

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confidence: 99%

Photodynamic therapy using intravenous δ-aminolaevulinic acid-induced protoporphyrin IX sensitisation in experimental hepatic tumours in rats

Svanberg

Liu²,

Wang³

et al. 1996

Br J Cancer

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Summary The efficacy of photodynamic therapy (PDT) using 6-aminolaevulinic acid (ALA)-induced protoporphyrin IX (PpIX) sensitisation and laser light at 635 nm was investigated in the treatment of experimental hepatic tumours. The model of liver tumours was induced either by local inoculation or by administration of tumour cells through the portal vein in rats. ALA at a dose of 60 mg kg-' b.w. was intravenously administered 60 min before PDT. PpIX accumulation in tumour, normal liver and abdominal wall muscle was detected by means of laser-induced fluorescence (LIF). Laser Doppler imaging (LDI) was used to determine changes in the superficial blood flow in connection with PDT. Histopathological examinations were performed to evaluate the PDT effects on the tumour and the surrounding liver tissue, including pathological features in the microvascular system. The accumulation of PpIX, as monitored by LIF, showed high fluorescence intensities at about 635 nm in both the hepatic tumour tissue and normal liver and low values in the abdominal wall. LDI demonstrated that the blood flow in the treated tumour and its surrounding normal liver tissue decreased immediately after the PDT, indicating an effect on the vascular system. A large number of thrombi in the irradiated tumour were found microscopically 3 h after the PDT. The tumour growth rate showed a marked decrease when evaluated 3 and 6 days after the treatment. These results show that the ALA-PDT is effective in the inhibition of growth of experimental hepatic tumours.

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“…Fluorescence-monitoring systems for endoscopic applications 2 ' 3 normally detect the total red fluorescence in a selected wavelength band. In a recent investigation, 4 ' 5 we demonstrated that, by monitoring the full laser-induced fluorescence (LIF) spectrum and by forming simple functions of fluorescence intensities in selected wavelength bands, it is possible to achieve a strong contrast enhancement in cancer-tumor detection, which is particularly valuable when a high level of natural HPD-nonrelated LIF is present. In this Letter we show how such procedures, which were used in point measurements, can be extended to the practically and clinically more interesting case of instantaneous imaging of the tissue area under consideration.…”

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confidence: 99%

“…4 A tumor on one hind leg of white Wistar-Furth rats, induced through subcutaneous in- killed and studied, they were intravenously injected with a HPD solution (Photofrin I, ORD Inc., Cheektowaaga, New York), normally at 5 mg/kg of body weight.…”

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confidence: 99%

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Multicolor imaging and contrast enhancement in cancer-tumor localization using laser-induced fluorescence in hematoporphyrin-derivative-bearing tissue

Montán

Svanberg

1985

Opt. Lett.

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Laser-Induced Fluorescence Studies of Hematoporphyrin Derivative (HPD) in Normal and Tumor Tissue of Rat

Cited by 62 publications

References 9 publications

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Photodynamic therapy using intravenous δ-aminolaevulinic acid-induced protoporphyrin IX sensitisation in experimental hepatic tumours in rats

Multicolor imaging and contrast enhancement in cancer-tumor localization using laser-induced fluorescence in hematoporphyrin-derivative-bearing tissue

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