Laser/LED phototherapy on the repair of tibial fracture treated with wire osteosynthesis evaluated by Raman spectroscopy

Abstract

The aim of the present study was to assess, by means of Raman spectroscopy, the repair of complete surgical tibial fractures fixed with wire osteosynthesis (WO) treated or not with infrared laser (λ780 nm) or infrared light emitting diode (LED) (λ850 ± 10 nm) lights, 142.8 J/cm per treatment, associated or not to the use of mineral trioxide aggregate (MTA) cement. Surgical tibial fractures were created on 18 rabbits, and all fractures were fixed with WO and some groups were grafted with MTA. Irradiated groups received lights at every other day during 15 days, and all animals were sacrificed after 30 days, being the tibia removed. The results showed that only irradiation with either laser or LED influenced the peaks of phosphate hydroxyapatite (~ 960 cm). Collagen (~ 1450 cm) and carbonated hydroxyapatite (~ 1070 cm) peaks were influenced by both the use of MTA and the irradiation with either laser or LED. It is concluded that the use of either laser or LED phototherapy associated to MTA cement was efficacious on improving the repair of complete tibial fractures treated with wire osteosynthesis by increasing the synthesis of collagen matrix and creating a scaffold of calcium carbonate (carbonated hydroxyapatite-like) and the subsequent deposition of phosphate hydroxyapatite.

A nontoxic strontium nanoparticle that holds the potential to act upon osteocompetent cells: An in vitro and in vivo characterization

“…Finally, the mineral structure deposited over the pPMMA cement was studied by Raman spectroscopy (Figure 8), which is an essential technique for determining the mineral crystal structure 64,65 . The RAMAN spectrum of the bone reveals the presence of characteristic bands at ~960 cm −1 (ν 1 PO 4 3− ) and ~ 1050 cm −1 , (ν 3 PO 4 3− ) stretching of hydroxyapatite as described previously 64–67 . The RAMAN spectra of the minerals deposited on the cement after implantation share similarities with the spectrum of bone, revealing tissue deposition on the biomaterial.…”

“…64,65 The RAMAN spectrum of the bone reveals the presence of characteristic bands at $960 cm À1 (ν 1 PO 4 3À ) and $ 1050 cm À1 , (ν 3 PO 4 3À ) stretching of hydroxyapatite as described previously. [64][65][66][67] The RAMAN spectra of the minerals deposited on the cement after implantation share similarities with the spectrum of bone, revealing tissue deposition on the biomaterial.…”

The Use of Near Infrared Light Emitting Diode for Kienbock’s Disease: A Case Study

“…With the addition of laser photo biomodulation, in the form of a low-level laser or LED, marked bone regeneration, integration, and decreased inflammation have been observed [19]. In the article lead by Pinheiro, beneficial outcomes were observed with the use of either a laser or LED by increasing collagen matrix creation [20].…”