Laser Microdissection

Frost, Andra R.; Eltoum, Isam; Siegal, Gene P.; Emmert‐Buck, Michael R.; Tangrea, Michael A.

doi:10.1002/0471142727.mb25a01s112

Cited by 12 publications

(4 citation statements)

References 99 publications

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“…The POL membrane slides with collected sections were dehydrated in a desiccation chamber with Drierite (with indicator) for 30–45 min as described (Morrison, Bailey, & Kulesa, 2012; Morrison, Box, McKinney, McLennan, & Kulesa, 2015). Laser microdissection (Frost, Eltoum, Siegal, Emmert‐Buck, & Tangrea, 2015) was performed using a Leica LMD 600 in the Cambridge Advanced Imaging Centre (University of Cambridge, Cambridge, UK).…”

Section: Methodsmentioning

confidence: 99%

Insights into olfactory ensheathing cell development from a laser‐microdissection and transcriptome‐profiling approach

Perera

Williams

Lyne

et al. 2020

Glia

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Olfactory ensheathing cells (OECs) are neural crest-derived glia that ensheath bundles of olfactory axons from their peripheral origins in the olfactory epithelium to their central targets in the olfactory bulb. We took an unbiased laser microdissection and differential RNA-seq approach, validated by in situ hybridization, to identify candidate molecular mechanisms underlying mouse OEC development and differences with the neural crest-derived Schwann cells developing on other peripheral nerves. We identified 25 novel markers for developing OECs in the olfactory mucosa and/or the olfactory nerve layer surrounding the olfactory bulb, of which 15 were OEC-specific (that is, not expressed by Schwann cells). One pan-OECspecific gene, Ptprz1, encodes a receptor-like tyrosine phosphatase that blocks oligodendrocyte differentiation. Mutant analysis suggests Ptprz1 may also act as a brake on OEC differentiation, and that its loss disrupts olfactory axon targeting. Overall, our results provide new insights into OEC development and the diversification of neural crest-derived glia.

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Section: Methodsmentioning

confidence: 99%

Insights into olfactory ensheathing cell development from a laser‐microdissection and transcriptome‐profiling approach

Perera

Williams

Lyne

et al. 2020

Glia

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“…Laser microdissection is a technique that utilizes a focused beam to isolate cell populations or individual cells from tissue sections under microscopic guidance [ 1 , 2 ]. Prior to laser microdissection, tissues need to be embedded into blocks before they are sectioned by microtome.…”

Section: Introductionmentioning

confidence: 99%

Laser axial scanning microdissection for high-efficiency dissection from uneven biological samples

Yang,

Ji,

Luo

et al. 2024

Biomed. Opt. Express

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Fast and efficient separation of target samples is crucial for the application of laser-assisted microdissection in the molecular biology research field. Herein, we developed a laser axial scanning microdissection (LASM) system with an 8.6 times extended depth of focus by using an electrically tunable lens. We showed that the ablation quality of silicon wafers at different depths became homogenous after using our system. More importantly, for those uneven biological tissue sections within a height difference of no more than 19.2 µm, we have demonstrated that the targets with a size of microns at arbitrary positions can be dissected efficiently without additional focusing and dissection operations. Besides, dissection experiments on various biological samples with different embedding methods, which were widely adopted in biological experiments, also have shown the feasibility of our system.

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“…And several different collection methods have been developed combined with laser microdissection. Contact collection methods require customized tube caps, including the silicone-filled adhesive cap and the transfer cap covered with a layer of thermoplastic film [ 15 ]. Compared with contact collection, non-contact collection has greater potential to avoid pollution.…”

Section: Introductionmentioning

confidence: 99%

Picosecond laser capture microdissection based on edge catapulting combined with dielectrophoretic force

Yang,

Liu,

Huang

et al. 2024

Biomed. Opt. Express

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The spatial omics information analysis of heterogeneous cells or cell populations is of great importance for biomedical research. Herein, we proposed a picosecond laser capture microdissection boosted by edge catapulting combined with dielectrophoretic force (ps-LMED) that enables fast and non-invasive acquisition of uncontaminated cells and cell populations for downstream molecular assays. The target cells were positioned under a microscope and separated by a focused picosecond pulsed laser. The system employed the plasma expansion force during cutting to lift the target and captured it under dielectrophoretic force from the charged collection cap eventually. The principle of our system has been validated by both theoretical analysis and practical experiments. The results indicated that our system can collect samples ranging from a single cell with a diameter of a few microns to large tissues with a volume of 532,500 µm3 at the moment finishing the cutting, without further operations. The cutting experiments of living cells and ribonucleic acid (RNA) and protein omics analysis results of collected targets demonstrated the advantage of non-destructiveness to the samples and feasibility in omics applications.

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Laser Microdissection

Cited by 12 publications

References 99 publications

Insights into olfactory ensheathing cell development from a laser‐microdissection and transcriptome‐profiling approach

Insights into olfactory ensheathing cell development from a laser‐microdissection and transcriptome‐profiling approach

Laser axial scanning microdissection for high-efficiency dissection from uneven biological samples

Picosecond laser capture microdissection based on edge catapulting combined with dielectrophoretic force

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