Laser microporation facilitates topical drug delivery: a comprehensive review about preclinical development and clinical application

Zhao, Yong; Voyer, Jewel; Li, Yibo; Kang, Xinliang; Chen, Xinyuan

doi:10.1080/17425247.2023.2152002

Cited by 12 publications

(11 citation statements)

References 106 publications

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“…Varying the pulse energies of the laser also did not affect the outcomes significantly. This is in line with previous studies showing that while higher pulse energies influence the depth of the micropores, the total drug delivery does not necessarily increase. , Both laser ablation and MN have well-documented clinical safety profiles triggering no or minor local reactions such as itching or redness. − The skin barrier function typically regenerates within a few hours , while pore closure occurs within 24–48 h. , Further, phase 3 clinical trials did not show any elevated infection risks following pore induction …”

Section: Results and Discussionsupporting

confidence: 88%

Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe In Situ Gene Editing in Human Skin

Bolsoni,

Liu,

Mohabatpour

et al. 2023

ACS Nano

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Despite exciting advances in gene editing, the efficient delivery of genetic tools to extrahepatic tissues remains challenging. This holds particularly true for the skin, which poses a highly restrictive delivery barrier. In this study, we ran a head-to-head comparison between Cas9 mRNA or ribonucleoprotein (RNP)-loaded lipid nanoparticles (LNPs) to deliver gene editing tools into epidermal layers of human skin, aiming for in situ gene editing. We observed distinct LNP composition and cell-specific effects such as an extended presence of RNP in slow-cycling epithelial cells for up to 72 h. While obtaining similar gene editing rates using Cas9 RNP and mRNA with MC3-based LNPs (10−16%), mRNA-loaded LNPs proved to be more cytotoxic. Interestingly, ionizable lipids with a pK a ∼ 7.1 yielded superior gene editing rates (55%−72%) in two-dimensional (2D) epithelial cells while no single guide RNA-dependent off-target effects were detectable. Unexpectedly, these high 2D editing efficacies did not translate to actual skin tissue where overall gene editing rates between 5%−12% were achieved after a single application and irrespective of the LNP composition. Finally, we successfully base-corrected a disease-causing mutation with an efficacy of ∼5% in autosomal recessive congenital ichthyosis patient cells, showcasing the potential of this strategy for the treatment of monogenic skin diseases. Taken together, this study demonstrates the feasibility of an in situ correction of disease-causing mutations in the skin that could provide effective treatment and potentially even a cure for rare, monogenic, and common skin diseases.

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Section: Results and Discussionsupporting

confidence: 88%

Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe In Situ Gene Editing in Human Skin

Bolsoni,

Liu,

Mohabatpour

et al. 2023

ACS Nano

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“…Lasers act to enhance the transdermal permeation of drugs through three primary mechanisms: (i) direct ablation, where drug passage is facilitated through the creation of microchannels in the skin; (ii) photothermal ablation, where the water and other components in the skin are heated through the absorption of the laser energy, resulting in small burns and disruption of the microstructure of the skin; and (iii) through the creation of photomechanical waves, where mechanical waves are created through exposure of a material in contact with the skin, creating transient pores within the intercellular lipid layer of the SC [105,106]. There are a number of different types of lasers used to facilitate TD, with some common types being the carbon dioxide (CO 2 ) laser and the erbium-doped yttrium aluminium garnet (Er:YAG) laser [107]. Low-energy laser ablation has been used to enhance the TD of diclofenac through rat skin in vivo [108].…”

Section: Laser Ablationmentioning

confidence: 99%

Strategies to Improve the Transdermal Delivery of Poorly Water-Soluble Non-Steroidal Anti-Inflammatory Drugs

Balmanno,

Falconer,

Ravuri

et al. 2024

Pharmaceutics

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The transdermal delivery of non-steroidal anti-inflammatory drugs (NSAIDs) has the potential to overcome some of the major disadvantages relating to oral NSAID usage, such as gastrointestinal adverse events and compliance. However, the poor solubility of many of the newer NSAIDs creates challenges in incorporating the drugs into formulations suitable for application to skin and may limit transdermal permeation, particularly if the goal is therapeutic systemic drug concentrations. This review is an overview of the various strategies used to increase the solubility of poorly soluble NSAIDs and enhance their permeation through skin, such as the modification of the vehicle, the modification of or bypassing the barrier function of the skin, and using advanced nano-sized formulations. Furthermore, the simple yet highly versatile microemulsion system has been found to be a cost-effective and highly successful technology to deliver poorly water-soluble NSAIDs.

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“…The drug delivery capacity of implantable materials is an important factor in improving their performance. For effective drug delivery, the introduction of mediators such as drug carriers and hydrogels or physical methods such as creating micropores on a material surface have been investigated [5][6][7][8]. In particular, to store and deliver drugs, it has been reported that micropores can be formed on the material surface using laser-based physical surface modification methods such as ion beam and femtosecond laser irradiation or chemical surface modification methods such as anodic oxidation and polishing [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Drug release control and anti-inflammatory effect of biodegradable polymer surface modified by gas phase chemical functional reaction

Bae,

Kim

2024

Biomed. Mater.

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The plasma technique has been widely used to modify the surfaces of materials. The purpose of this study was to evaluate the probability of controlling the prednisolone delivery velocity on a polylactic acid (PLA) surface modified by plasma surface treatment. Surface modification of PLA was performed at a low-pressure radio frequency under conditions of 100 W power, 50 mTorr chamber pressure, 100~200 sccm of flow rate, and Ar, O2, and CH4 gases. The plasma surface-modified PLA was characterized using scanning emission microscope, X-ray photoelectron spectroscopy (XPS), and contact angle measurements. In vitro evaluations were performed to determine cellular response, drug release behavior, and anti-inflammatory effects. The PLA surface morphology was changed to a porous structure (with a depth of approximately 100 μm) and the surface roughness was also significantly increased. The XPS results demonstrated higher oxygenized carbon contents than those in the non-treated PLA group. The prednisolone holding capacity increased and the release was relatively prolonged in the surface-modified PLA group compared to that in the non-treated PLA group. In addition, cell migration and proliferation significantly increased after PLA treatment alone. The activity of cytokines such as COX-2, TNF-α, IL-1β, and IL-6 were considerably reduced in the plasma-treated and prednisolone holding group. Taken together, surface-modified PLA by plasma can provide an alternative approach to conventional physicochemical approaches for sustained anti-inflammatory drug release.

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Laser microporation facilitates topical drug delivery: a comprehensive review about preclinical development and clinical application

Cited by 12 publications

References 106 publications

Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe In Situ Gene Editing in Human Skin

Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe In Situ Gene Editing in Human Skin

Strategies to Improve the Transdermal Delivery of Poorly Water-Soluble Non-Steroidal Anti-Inflammatory Drugs

Drug release control and anti-inflammatory effect of biodegradable polymer surface modified by gas phase chemical functional reaction

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