Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging

Abstract

The visualization of optical phase can provide abundant information when imaging transparent specimen. We present a novel phase sensitive imaging design capable of obtaining phase contours of transparent biological cells through laser oblique scanning optical microscope (LOSOM). LOSOM is based on the introduction of a fluorescent medium behind the specimen to generate a differential phase-sensitive image, thus, the complicated phase retardation alignment procedure associated with differential interference contrast (DIC) microscopy can be eliminated. Moreover, multi-modality fluorescence and phase relief imaging can be attained in a single system with fluorescently labeled specimens.

Integrating light-sheet imaging with virtual reality to recapitulate developmental cardiac mechanics

“…Integration of Bessel beam (33) and 2-photon excitation (34) with our current cardiac LSFM will lead to better spatial resolution. Other imaging modalities (42)(43)(44)(45)(46), such as photoacoustic, computed tomography, and magnetic resonance imaging, are compatible with our developing workflow for the future translational VR platform of diagnosis and therapy. The methodology used for developing the VR-LSFM application will also be transferable to other VR head-mounted devices for enhancing user-interactivity features and improving the quantitative analysis.…”

Application of Low-Dose Laser Irradiation and 3D-Observation Methods for Detection and Assay of Features for Cell Differentiation Directions: A Pilot Study

“…So, unlike the 2D-images in white light are due to differences only in the volume of the tested objects due to absorption, the 3D-imaginations, have been proved to also indicate variations in their refractive index, which leads to changed phase of the light. [16][17][18][19][20]24 EXPERIMENTAL DESIGN Derivation of various normal mature cell lineages by appropriate incubation of cell progenitors with common origin: Mouse embryonic fibroblasts from line 3T3 are incubated in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma), supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS) (Sigma) and antibiotic mixture (100 IU/ml Penicillin, 0.25 mg/ml Streptomycin and 0.25 mg/ml Amphothericin) (Sigma), until formation of confluent monolayers, at 37 0 C in incubator with 5% CO2 and 95% humidity. Malignant mouse myeloma cells were also isolated, similarly incubated.…”

Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy

“…Alternatively, a scanning technique has been demonstrated based on oblique illumination produced by subsurface fluorescence. 24 We recently introduced oblique back-illumination microscopy (OBM) 25 as a fast phase-gradient contrast technique for imaging lateral index of refraction variations in thick, scattering tissues using incoherent illumination and standard widefield detection with a camera. OBM provides phase-gradient contrast based on transillumination through the focal plane of interest, even though it is configured in a reflection geometry.…”