Laser Printing of Pluripotent Embryonal Carcinoma Cells

Ringeisen, Bradley R.; Kim, Heung Soo; Barron, Jason A.; Krizman, David B.; Chrisey, Douglas B.; Jackman, Shawna M; Auyeung, R. Y. C.; Spargo, Barry J.

doi:10.1089/107632704323061843

Cited by 269 publications

(169 citation statements)

References 33 publications

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“…LIFT is an established technique for cell deposition, with postprinting cell viability reaching > 95% [40,41]. Furthermore, previous studies showed that cell suspensions could withstand laser-induced acceleration and deceleration of the order of 10 6 −10 7 g and authors speculated that only the cells at the front of the jet were damaged [42,43]. By analogy, our laser-assisted system could potentially be viable for sensitive cell-seeded liquids as we estimate from time-resolved imaging that the maximum deceleration at impact is of the order of 10 6 g.…”

Section: Application To Particle Deliverymentioning

confidence: 99%

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

et al. 2018

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Needle-free jet injection enables the delivery of drugs into skin or soft tissue by puncturing them with a high-velocity liquid jet. However, precise and efficient drug delivery requires generating such liquid jets with both a controlled velocity and a high throughput, which remains challenging with current spring-and gas-actuated jet injectors. Here, we propose a depth-controlled and high-throughput injection method by adapting laser-induced forward transfer (LIFT), a high-resolution two-dimensional printing technique, for direct three-dimensional liquid delivery into soft tissues.The velocity of thin liquid jets is laser actuated from 10 to 85 m/s so that doses as small as 10 pL, not achievable with other injectors, are injected at a 1 Hz repetition rate into a 300 μm thick soft gelatin substrate with a 25 μm depth precision and 12 μm lateral resolution. We further investigate the potential of this liquid delivery technique as a direct three-dimensional cell-delivery vehicle and show that depth-controlled particle delivery requires high-delivery efficiency. Our direct three-dimensional liquid delivery system opens up more possibilities for pinpoint drug delivery in soft tissues or tissue-engineered constructs.

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Section: Application To Particle Deliverymentioning

confidence: 99%

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

et al. 2018

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“…[67][68][69] The repetition rate of the laser (5 kHz) has showed high cell density (10 8 cells/mL), high-speed (200 mm/s), and highresolution (10 lm) patterning with multiple cell seeding on the scaffolds. 21 Gaebel et al compared the benefit of LIFT-based versus non-LIFT-based tissue engineered cardiac patches for the potential treatment of myocardial infarction.…”

Section: Biomaterials Printing Using Laser Induced Forward Transfermentioning

confidence: 99%

Laser-assisted biofabrication in tissue engineering and regenerative medicine

et al. 2016

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Controlling the spatial arrangement of biomaterials and living cells provides the foundation for fabricating complex biological systems. Such level of spatial resolution (less than 10 lm) is difficult to be obtained through conventional cell processing techniques, which lack the precision, reproducibility, automation, and speed required for the rapid fabrication of engineered tissue constructs. Recently, laserassisted biofabrication techniques are being intensively developed with the use of computer-aided processes for patterning and assembling both living and nonliving materials with prescribed 2D or 3D organization. In this review, we discuss laser-assisted fabrication methods, including laser tweezers, multi-photon polymerization, laser-induced forward transfer (LIFT), matrix assisted pulsed laser evaporation (MAPLE), and laser ablation as well as their applications in biological science and biomedical engineering. These advanced technologies enable the precise manipulation of in vitro cellular microenvironments and the ability to engineer functional tissue constructs with high complexity and heterogeneity, which serve in regenerative medicine, pharmacology, and basic cell biology studies.

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“…Further work enhanced the cell printing capabilities, showing that various cell types including human osteosarcoma and rat cardiac cells (Barron et al 2004b, Ringeisen et al 2004, could be printed with viability approaching 100% at near single-cell resolution using Matrigel™ as the absorptive layer. At the same time, this group developed the improved BioLP™ approach in an attempt to limit direct interaction between the laser and sensitive biomaterials (Barron et al 2004a).…”

Section: Current Progress Chrisey Et Al First Demonstrated the Maplementioning

confidence: 99%

Biofabrication: an overview of the approaches used for printing of living cells

Ferris

Gilmore

Wallace

et al. 2013

Appl Microbiol Biotechnol

210

132

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The development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable and reproducible printing of cells. This review outlines the general principles and current progress and compares the advantages and challenges for the most widely used biofabrication techniques for printing cells: extrusion, laser, microvalve, inkjet and tissue fragment printing. It is expected that significant advances in cell printing will result from synergistic combinations of these techniques and lead to optimised resolution, throughput and the overall complexity of printed constructs. AbstractThe development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable, reproducible printing of cells. This review outlines the general principles, current progress, and compares the advantages and challenges for the most widely used biofabrication techniques for printing cells: extrusion, laser, microvalve, inkjet and tissue fragment printing. It is expected that significant advances in cell printing will result from synergistic combinations of these techniques and lead to optimised resolution, throughput and the overall complexity of printed constructs.

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Laser Printing of Pluripotent Embryonal Carcinoma Cells

Cited by 269 publications

References 33 publications

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

Laser-assisted biofabrication in tissue engineering and regenerative medicine

Biofabrication: an overview of the approaches used for printing of living cells

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