Laser speckle imaging based on intensity fluctuation modulation

Zeng, Yaguang; Wang, Mingyi; Gao, Feng; Liang, Xianjun; Yang, Guojian

doi:10.1364/ol.38.001313

Cited by 35 publications

(31 citation statements)

References 17 publications

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“…Note that this AIFM imaging process is similar to our previous work on IFM imaging with laser speckle effect . Using fast Fourier transform (FFT) to transfer the raw temporal signal recorded at each pixel from the time domain to the frequency domain.…”

Section: Materials and Theorymentioning

confidence: 99%

In vivo full‐field functional optical hemocytometer

Zhang

Wang

Han

et al. 2017

Journal of Biophotonics

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We present an in vivo lab-free full-field functional optical hemocytometer (FFOH) for application to the capillaries of a live biological specimen, based on the absorption intensity fluctuation modulation (AIFM) effect. Because of the absorption difference between the red blood cells (RBCs) and background tissue under low-coherence light illumination, an endogenous instantaneous intensity fluctuation is generated by the AIFM effect when RBCs discontinuously traverse the capillary. The AIFM effect is used to highlight the RBC signal relative to the background tissue by computing the real-time modulation depth. FFOH can simultaneously provide a flow video, the flow velocity and the RBC count. Ourexperimental results can potentially be applied to study the physiological mechanisms of the blood circulation systems of near-transparent live biological samples.

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Section: Materials and Theorymentioning

confidence: 99%

In vivo full‐field functional optical hemocytometer

Zhang

Wang

Han

et al. 2017

Journal of Biophotonics

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“…To this end, we use intensity fluctuation modulation (IFM), which was first proposed in laser speckle angiography. 16,17 The main idea of IFM is that the information of blood flow can be obtained by separating the dynamic signal, which is related to the moving RBCs in the blood flow, from the stationary signal, which is related to the background tissue. In the present case, the dynamic signal is just I RBC ðtÞ and the stationary signal is I 0 in Eq.…”

Section: Red Blood Cell Length Calculationmentioning

confidence: 99%

Full-field velocity imaging of red blood cells in capillaries with spatiotemporal demodulation autocorrelation

et al. 2016

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We propose a full-field optical method for the label-free and quantitative mapping of the velocities of red blood cells (RBCs) in capillaries. It integrates spatiotemporal demodulation and an autocorrelation algorithm, and measures RBC velocity according to the ratio of RBC length to lag time. Conventionally, RBC length is assumed to be a constant and lag time is taken as a variable, while our method treats both of them as variables. We use temporal demodulation and the Butterworth spatial filter to separate RBC signal from background signal, based on which we obtain the RBC length by image segmentation and lag time by autocorrelation analysis. The RBC velocity calculated now is more accurate. The validity of our method is verified by an in vivo experiment on a mouse ear. Owing to its higher image signal-to-noise ratio, our method can be used for mapping RBC velocity in the turbid tissue case.

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“…In the present study, we achieved wide-field centerline ATFV measurement in a vessel based on the absorption intensity fluctuation modulation (AIFM) effect. 11 Because of the difference in absorption between the RBCs and the background tissue under low-coherence light illumination at a center wavelength of 540 nm, 12 an endogenous instantaneous intensity fluctuation is generated by the AIFM effect when RBCs discontinuously traverse the blood vessel. The AIFM effect is used to obtain the wide-field optical angiography image and to highlight the RBCs' signal relative to the background tissue by computing the average modulation depth (AMD) and the instantaneous modulation depth (IMD).…”

Section: Introductionmentioning

confidence: 99%

Wide-field absolute transverse blood flow velocity mapping in vessel centerline

Wang

Zhu

et al. 2018

J. Biomed. Opt.

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We propose a wide-field absolute transverse blood flow velocity measurement method in vessel centerline based on absorption intensity fluctuation modulation effect. The difference between the light absorption capacities of red blood cells and background tissue under low-coherence illumination is utilized to realize the instantaneous and average wide-field optical angiography images. The absolute fuzzy connection algorithm is used for vessel centerline extraction from the average wide-field optical angiography. The absolute transverse velocity in the vessel centerline is then measured by a cross-correlation analysis according to instantaneous modulation depth signal. The proposed method promises to contribute to the treatment of diseases, such as those related to anemia or thrombosis.

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Laser speckle imaging based on intensity fluctuation modulation

Cited by 35 publications

References 17 publications

In vivo full‐field functional optical hemocytometer

In vivo full‐field functional optical hemocytometer

Full-field velocity imaging of red blood cells in capillaries with spatiotemporal demodulation autocorrelation

Wide-field absolute transverse blood flow velocity mapping in vessel centerline

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