Laser trapping ionization of single human red blood cell

Pasquerilla, M; Kelley, Michele; Mushi, Robert; Aguinaga, Maria del Pilar; Erenso, Daniel

doi:10.1088/2057-1976/aac57d

Cited by 8 publications

(26 citation statements)

References 16 publications

(26 reference statements)

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“…In TIE and TRD studies for single cell, during the trapping process, we had to keep the cell isolated from the other cells until it is fully ionized and ejected from the trap. This was challenging as the ionization period is relatively longer for cancer cells compared to other small cells such as RBCs 18–20 . During this period, other nearby cells are attracted by the strong gradient force and jump into the trap at different times, which results in having multiple cells in the trap.…”

Section: Resultsmentioning

confidence: 99%

“…1. This experimental set-up is very similar to the set-up used in previous biomedical laser trapping application studies 18–20 . Thus, here we briefly discuss the basic elements of this set-up relevant to our study.…”

Section: Methodsmentioning

confidence: 94%

“…The TRD was then calculated for each of the 4T1 cells usinghere the TIE is the minimum radiation energy absorbed by the cell that creates a significant amount of charge through ionization that leads to irreversible damage to the cell. When the cell reaches this threshold charge, it experiences a stronger electrostatic force than the trapping force that results in the ejection of the cell from the trap 18–20 .…”

Section: Methodsmentioning

confidence: 99%

“…This study uses a new approach that uses laser trapping technique (LT) 17 for single cell ionization. We have recently demonstrated this approach in BT20 breast cancer cells 18 and human red blood cells 19,20 . This study uses a high-power infrared laser (at 1064 nm) to measure the radio sensitivity of 4T1 breast carcinoma cell lines treated by a naturally occurring compound, 2-Dodecyl-6-methoxycyclohexa-2, 5-diene-1, 4-dione (DMDD) extracted from the root of Averrhoa carambola L. Studies have shown that DMDD induces apoptosis of various human breast cancer cells through production of intracellular reactive oxygen species (ROS) and inhibition of NF-κB activation 21 .…”

Section: Introductionmentioning

confidence: 99%

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Chemo-treated 4T1 breast cancer cells radiation response measured by single and multiple cell ionization using infrared laser trap

Muhammed

Chen

Gao

et al. 2019

Sci Rep

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We present a study that uses a laser trapping technique for measurement of radiation sensitivity of untreated and chemo-treated cancer cells. We used a human mammary tumor cell line (4T1) treated by an antitumor compound, 2-Dodecyl-6-methoxycyclohexa-2, 5-diene-1,4-dione (DMDD), which was extracted from the root of Averrhoa carambola L. The untreated control group, and both 2-hour and 24-hour treated groups of 4T1 cells were used in this study. The absorbed threshold ionization energy (TIE) and the threshold radiation dose (TRD) were determined using a high-power infrared laser (at 1064 nm) trap by single and multiple cells trapping and ionization. The results were analyzed using descriptive and t-statistics. The relation of the TIE and TRD to the mass of the individual cells were also analyzed for different hours of treatment in comparison with the control group. Both TIE and TRD decrease with increasing treatment periods. However, the TRD decreases with mass regardless of the treatment. Analyses of the TRD for single vs multiple cells ionizations within each group have also consistently showed this same behavior regardless of the treatment. The underlying factors for these observed relations are explained in terms of radiation, hyperthermia, and chemo effects.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chemo-treated 4T1 breast cancer cells radiation response measured by single and multiple cell ionization using infrared laser trap

Muhammed

Chen

Gao

et al. 2019

Sci Rep

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“…Another study demonstrates the duration of the post-optical tweezers exposure effect on RBC morphology and its membrane rigidness in adult blood [373] and umbilical cord blood [374]. Lastly, the aspects of single RBC ionization using laser-trapped techniques have also been considered, highlighting the potentials for measuring hemoglobin charges and other biophysical properties [375][376][377].…”

Section: Optical Trappingmentioning

confidence: 99%

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

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