Laser Tweezer Deformation of Giant Unilamellar Vesicles

Poole, Cory; Mészáros, József; Senthil, K.; Losert, Wolfgang

doi:10.1385/1-59745-519-9:389

Cited by 2 publications

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“…GUV Preparation : GUVs used for Raman analysis were prepared by electroformation, essentially following the procedure described in ref. . Briefly, DOPC (by Avanti) dissolved in a 10:1 chloroform to methanol solution was spread over the conducting side of an indium tin oxide (ITO) slide and desiccated under reduced pressure.…”

Section: Methodsmentioning

confidence: 99%

Selective Localization of Hierarchically Assembled Particles to Plasma Membranes of Living Cells

et al. 2019

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Particles that preferentially partition to a specific cellular subunit, such as the nucleus, mitochondria, or the cytoskeleton, are of relevance to a number of applications, including drug delivery, genetic manipulation, or self‐assembly. Here, hierarchical assemblies of fully synthetic particles that selectively localize to the plasma membrane of mammalian cells are presented. A multimodal approach is used to create assemblies of polymer‐based carrier particles with amphiphilic gold nanoparticles immobilized on one hemisphere. These assemblies persist in the plasma membrane of cells for several days and undergo rearrangements and clustering, typically considered to be hallmarks of membrane‐bound receptors.

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Section: Methodsmentioning

confidence: 99%

Selective Localization of Hierarchically Assembled Particles to Plasma Membranes of Living Cells

et al. 2019

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“…Later, the same group used an AFM tip to apply indentations in GUVs . Other methods to induce morphological changes in giant vesicles include contact‐induced vesicle deformation, high pressure, dual optical beams, optically trapped beads, aqueous wetting, light irradiation, magnetic fields, and electric fields …”

Section: Introductionmentioning

confidence: 99%

Observations of Membrane Domain Reorganization in Mechanically Compressed Artificial Cells

Robinson

Dittrich

2019

ChemBioChem

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Giant unilamellar vesicles (GUVs) are considered to be the gold standard for assembling artificial cells from the bottom up. In this study, we investigated the behavior of such biomimetic vesicles as they were subjected to mechanical compression. A microfluidic device is presented that comprises a trap to capture GUVs and a microstamp that is deflected downwards to mechanically compress the trapped vesicle. After characterization of the device, we show that single‐phase GUVs can be controllably compressed to a high degree of deformation (D=0.40) depending on the pressure applied to the microstamp. A permeation assay was implemented to show that vesicle bursting is prevented by water efflux. Next, we mechanically compressed GUVs with co‐existing liquid‐ordered and liquid‐disordered membrane phases. Upon compression, we observed that the normally stable lipid domains reorganized themselves across the surface and fused into larger domains. This phenomenon, observed here in a model membrane system, not only gives us insights into how the multicomponent membranes of artificial cells behave, but might also have interesting consequences for the role of lipid rafts in biological cells that are subjected to compressive forces in a natural environment.

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Laser Tweezer Deformation of Giant Unilamellar Vesicles

Cited by 2 publications

References 0 publications

Selective Localization of Hierarchically Assembled Particles to Plasma Membranes of Living Cells

Selective Localization of Hierarchically Assembled Particles to Plasma Membranes of Living Cells

Observations of Membrane Domain Reorganization in Mechanically Compressed Artificial Cells

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