LASP-1, a Novel Urinary Marker for Detection of Bladder Cancer

Ardelt, Peter; Grünemay, Nadine; Strehl, Annette; Jilg, Cordula A.; Miernik, A.; Kneitz, Burkhard; Butt, Elke

doi:10.1016/j.urolonc.2012.02.002

Cited by 21 publications

(15 citation statements)

References 21 publications

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“…Our results taken together with these previous observations suggest that higher expression of LASP-1 is involved in the development of metastatic phenotype. This is supported by the recent observation that LASP-1 can be a promising urinary marker for detection of bladder cancer (13,14).…”

Section: Discussionsupporting

confidence: 57%

“…Several groups reported that overexpression of LASP-1 has been observed in a variety of malignant tumors including metastatic breast cancer (10), ovarian cancer (11) and medulloblastoma (12). Recent clinical studies have demonstrated that LASP is one of the useful biomarkers for bladder cancer detection (13,14) and that LASP-1 is involved in bladder cancer metastasis (15). The above studies on the LASP-1 function and its high expression in malignant bladder cancer led us to postulate that LASP-1 is involved in bladder cancer malignant phenotypes such as cell invasion through the regulation of actin cytoskeleton.…”

Section: Introductionmentioning

confidence: 99%

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The role of LIM and SH3 protein-1 in bladder cancer metastasis

Sato¹,

Yoneyama²,

Hatakeyama

et al. 2017

Oncology Letters

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Abstract. The LIM and SH3 protein-1 (LASP-1) is a multi-domain protein that is involved in several malignant cancers. The role of LASP-1 in malignant phenotypes including high invasive properties and unrestricted cell proliferation, remain to be elucidated. The present study reported the association of LASP-1 expression with bladder cancer malignancy and its role in cancer cell invasion and proliferation. The immunohistochemical analysis of the expression status of LASP-1 in radical cystectomy specimens from invasive bladder cancer patients revealed that the LASP-1-positive patients demonstrated a decreased survival rate compared with the LASP-1-negative patients. The expression level of LASP-1 was increased in invasive bladder cancer cell lines compared with the non-invasive bladder cancer cell lines. Invasive cancer cells form invadopodia, the filamentous actin-based membrane protrusions that are essential in cancer cell invasion. Knockdown of LASP-1 reduced the ability to form invadopodia, resulting in decreased invasive capacity of the LASP-1 knockdown cells. In addition, knockdown of LASP-1 reduced cell proliferation. These results suggest that LASP-1 is important in invadopodia formation and cell proliferation of bladder cancer cells, promoting the malignant properties and resulting in poor-prognosis.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

The role of LIM and SH3 protein-1 in bladder cancer metastasis

Sato¹,

Yoneyama²,

Hatakeyama

et al. 2017

Oncology Letters

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“…LIM and SH3 domain protein 1 (LASP‐1) is a component of focal adhesions, lamellipodia, membrane ruffles, and pseudopodia in normal cells that participates in cellular adhesion and migration via interactions with different cytoskeletal proteins (Orth, Cazes, Butt, & Grunewald, ). Recently, overexpression of LASP‐1 was found to contribute to various cancers, including breast carcinoma (Grunewald et al, ), prostate carcinoma (Nishikawa et al, ), colorectal carcinoma (Niu et al, ), bladder carcinoma (Ardelt et al, ), gallbladder cancer (Li et al, ). In addition, LASP‐1 might serve as a clinical prognostic marker that can be identified by immunohistochemistry (IHC) in different tumors such as breast cancer and medulloblastoma (Frietsch et al, ; Traenka et al, ).…”

Section: Introductionmentioning

confidence: 99%

HBX protein promotes LASP‐1 expression through activation of c‐Jun in human hepatoma cells

You

Kong²,

Zhou³

et al. 2018

Journal Cellular Physiology

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LIM and SH3 domain protein 1 (LASP-1) is known to participate in the progression of hepatocellular carcinoma (HCC). We previously showed that ectopic expression of hepatitis B virus (HBV) X protein (HBX) enhanced the expression of LASP-1, which promoted proliferation and migration of HCC cells. Here, we further demonstrated the molecular mechanism underlying upregulation of LASP-1, mediated by HBX, in HBV-infected HCC cells. Through a luciferase activity assay, we discovered that the LASP-1 promoter region regulated by HBX contained an AP-1 binding element in human hepatoma cells. Interestingly, c-Jun, one subunit of AP-1, was mainly responsible for activation, mediated by HBX, of the LASP-1 promoter. Furthermore, HBX was shown not only to interact with phosphorylated c-Jun in HCC cells but also to activate c-Jun by increasing the activation of PI3-K/JNK signaling. Chromatin immunoprecipitation (ChIP) assay demonstrated that HBX was capable of binding to the LASP-1 promoter with c-Jun. Further, the expression levels of HBX were shown to be significantly positively correlated with that of LASP-1 and phosphorylatedc-Jun in HBV-related HCC tissues by immunohistochemistry analysis. In addition, the N-terminus of HBX was found to be responsible for the activation of c-Jun, as well as the expression of LASP-1. Taken together, these results suggest that HBX contributes to LASP-1 expression via the activation of c-Jun to increase the promoter activity of LASP-1 in HBV-related HCC cells.

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“…But the two methods also have shortcomings [32], they are costly, unpleasant, time consuming and trained personnel requirement [33]. At present, it is still a hot point to find biomarkers for the diagnosis of bladder cancer [34,35]. HA is known to promote tumor metastasis and help avoid immune surveillance by forming a protective coat around the tumor cells [36].…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic acid/ Hyaluronidase as biomarkers for bladder cancer: a diagnostic meta-analysis

Liang¹,

Zhang²,

Ch³

et al. 2017

neo

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This study aimed to determine the value of HA/HAase for detecting bladder cancer on the basis of preceding statistical performance. PubMed, Springer Link, Web of Science and Cochrane Library were systematically searched to identify potentially relevant published articles by using the key words: "bladder cancer or bladder tumor or bladder carcinoma", "hyaluronic acid or hyaluronan", "hyaluronidase or HAase". The methodological quality of each study was assessed by QUADAS-2. According to the inclusive and exclusive criteria, 8 articles were identified and methodologically analyzed by STATA 12.0 software package.The results showed that the pooled sensitivity of HA and HAase was 0.832 (95% confidence interval [CI]: 0.798, 0.861) and 0.834 (95% CI: 0.756, 0.891) respectively, the pooled specificity was 0.886 (95% CI: 0.852, 0.913) and 0.860 (95% CI: 0.801, 0.904), and the area under the summary ROC cure (AUC) was 0.90 (95% CI: 0.87, 0.92) and 0.91 (95% CI: 0.88, 0.93), respectively. Simultaneously the diagnostic accuracy of the combination of HA and HAase showed that the pooled sensitivity was 0.908 (95% CI: 0.879, 0.931), the pooled specificity was 0.825 (95% CI: 0.789, 0.856) and AUC was 0.94 (95% CI: 0.91, 0.95), indicating a relatively higher accuracy than HA and HAase alone. This meta-analysis strongly suggests that HA/HAase could be used as biomarkers for the diagnosis of bladder cancer.

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LASP-1, a Novel Urinary Marker for Detection of Bladder Cancer

Cited by 21 publications

References 21 publications

The role of LIM and SH3 protein-1 in bladder cancer metastasis

The role of LIM and SH3 protein-1 in bladder cancer metastasis

HBX protein promotes LASP‐1 expression through activation of c‐Jun in human hepatoma cells

Hyaluronic acid/ Hyaluronidase as biomarkers for bladder cancer: a diagnostic meta-analysis

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