LASP-1—a promising urine marker for detection of bladder cancer

Payton, Sarah

doi:10.1038/nrurol.2012.84

Cited by 9 publications

(4 citation statements)

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“…Our results taken together with these previous observations suggest that higher expression of LASP-1 is involved in the development of metastatic phenotype. This is supported by the recent observation that LASP-1 can be a promising urinary marker for detection of bladder cancer (13,14).…”

Section: Discussionsupporting

confidence: 57%

“…Several groups reported that overexpression of LASP-1 has been observed in a variety of malignant tumors including metastatic breast cancer (10), ovarian cancer (11) and medulloblastoma (12). Recent clinical studies have demonstrated that LASP is one of the useful biomarkers for bladder cancer detection (13,14) and that LASP-1 is involved in bladder cancer metastasis (15). The above studies on the LASP-1 function and its high expression in malignant bladder cancer led us to postulate that LASP-1 is involved in bladder cancer malignant phenotypes such as cell invasion through the regulation of actin cytoskeleton.…”

Section: Introductionmentioning

confidence: 99%

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The role of LIM and SH3 protein-1 in bladder cancer metastasis

Sato¹,

Yoneyama²,

Hatakeyama

et al. 2017

Oncology Letters

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Abstract. The LIM and SH3 protein-1 (LASP-1) is a multi-domain protein that is involved in several malignant cancers. The role of LASP-1 in malignant phenotypes including high invasive properties and unrestricted cell proliferation, remain to be elucidated. The present study reported the association of LASP-1 expression with bladder cancer malignancy and its role in cancer cell invasion and proliferation. The immunohistochemical analysis of the expression status of LASP-1 in radical cystectomy specimens from invasive bladder cancer patients revealed that the LASP-1-positive patients demonstrated a decreased survival rate compared with the LASP-1-negative patients. The expression level of LASP-1 was increased in invasive bladder cancer cell lines compared with the non-invasive bladder cancer cell lines. Invasive cancer cells form invadopodia, the filamentous actin-based membrane protrusions that are essential in cancer cell invasion. Knockdown of LASP-1 reduced the ability to form invadopodia, resulting in decreased invasive capacity of the LASP-1 knockdown cells. In addition, knockdown of LASP-1 reduced cell proliferation. These results suggest that LASP-1 is important in invadopodia formation and cell proliferation of bladder cancer cells, promoting the malignant properties and resulting in poor-prognosis.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

The role of LIM and SH3 protein-1 in bladder cancer metastasis

Sato¹,

Yoneyama²,

Hatakeyama

et al. 2017

Oncology Letters

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“…genecards.org/cgi-bin/carddisp.pl?gene=LASP1, accessed on 10 October 2022). Since then, several authors have studied LASP1 expression, at the mRNA or protein level, in different cancer types [2][3][4][5][6][7][8], reporting that its overexpression is inversely correlated with poor prognosis in breast and prostate cancer, medulloblastoma and hepatocellular carcinoma (HCC). Concerning HCC, we have previously shown that LASP1 mRNA is significantly overexpressed in HCC tissues, mainly in HCC developed in cirrhotic liver, and that levels of LASP1 protein and mRNA expression are comparable and increase in recurrent HCC [9].…”

Section: Introductionmentioning

confidence: 99%

Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish

Grossi

Schiavone

Cannone

et al. 2022

Genes

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The LIM and SH3 domain protein 1 (LASP1) was originally identified in metastatic breast cancer and mainly characterized as a cytoskeleton protein overexpressed in various cancer types. At present, little is known about LASP1 expression in physiological conditions, and its function during embryonic development has not been elucidated. Here, we focused on Lasp1 and embryonic development, choosing zebrafish as a vertebrate model. For the first time, we identified and determined the expression of Lasp1 protein at various stages of development, at 48 and 72 h post-fertilization (hpf), at 6 days pf and in different organs of zebrafish adults by Western blotting, 3D light-sheet microscopy and fluorescent immunohistochemistry. Further, we showed that specific lasp1 morpholino (MO) led to (i) abnormal morphants with alterations in several organs, (ii) effective knockdown of endogenous Lasp1 protein and (iii) an increase in lasp1 mRNA, as detected by ddPCR. The co-injection of lasp1 mRNA with lasp1 MO partially rescued morphant phenotypes, thus confirming the specificity of the MO oligonucleotide-induced defects. We also detected an increase in apoptosis following lasp1 MO treatment. Our results suggest a significant role for Lasp1 in embryonic development, highlighting zebrafish as a vertebrate model suitable for studying Lasp1 function in developmental biology and organogenesis.

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“…LIM and SH3 protein 1 (Lasp-1) is a specific focal adhesion protein that serves an important role in the regulation of cell proliferation and migration ( 6 , 7 ). It has been reported to be upregulated in a number of malignant tumors, including clear cell renal ( 8 ), non-small cell lung ( 9 ), prostate ( 10 ), gallbladder ( 11 ), bladder ( 12 , 13 ), breast ( 14 ), hepatocellular ( 15 , 16 ) and colorectal ( 17 , 18 ) carcinomas. The role of Lasp-1 in metastasis and progression of colorectal carcinomas has been suggested previously ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway

Zhao

Liu

2018

Oncol Lett

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LIM and SH3 protein 1 (Lasp-1), a focal adhesion protein, serves a critical role in the regulation of cell proliferation and migration. The role of Lasp-1, as well as the intracellular signaling pathways involved in metastasis and progression of colorectal carcinoma, remains unclear. In the present study, the regulatory effect of Lasp-1 and the underlying molecular mechanism involved in migration and invasion of colorectal carcinoma were investigated. RNA interference and overexpression in SW480 cells were performed to elucidate the role of Lasp-1. Reverse transcription-quantitative polymerase chain reaction, western blotting and immunofluorescence were conducted to determine the mRNA and protein levels of Lasp-1 and extracellular-signal-regulated kinase 1/2 (ERK1/2) in SW480 cells as well as tumor and adjacent normal tissues obtained from 20 patients with colorectal carcinoma. Furthermore, a cell proliferation assay, flow cytometric analysis, and cell migration and invasion assays were performed to examine the effect of Lasp-1 on cell growth. The results of the present study demonstrated that Lasp-1 and ERK1/2 were upregulated in tumor tissue compared with adjacent normal colorectal mucosa. Cell-based in vitro assays demonstrated that Lasp-1 knockdown suppressed the expression and activation of ERK1/2, whereas Lasp-1 overexpression resulted in ERK1/2 upregulation. Additionally, Lasp-1 knockdown inhibited cell proliferation, migration, and invasion and induced cellular apoptosis and G0/G1 cell-cycle arrest. The results of the present study indicate that Lasp-1 serves a critical role in the progression of colorectal carcinoma regulating the activation of the mitogen-activated protein kinase signaling pathway.

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LASP-1—a promising urine marker for detection of bladder cancer

Cited by 9 publications

References 1 publication

The role of LIM and SH3 protein-1 in bladder cancer metastasis

The role of LIM and SH3 protein-1 in bladder cancer metastasis

Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish

LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway

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