Latanoprost-Loaded Nanotransfersomes Designed for Scalp Administration Enhance Keratinocytes Proliferation

Abstract

Latanoprost (LAT) has been shown to have a hypertrichotic effect, which makes it a promising candidate for alopecia treatments. For the first time, LAT has been encapsulated in nanotransfersomes in order to increase its efficacy. Ex vivo skin biodistribution was studied by confocal laser microscopy both in human scalp and pig skin. Results showed that nanotransfersomes increase the penetration of two different fluorochromes, with similar patterns in both species, compared with fluorochrome solutions containing no nanotransfersomes. Nanotransfersomes were stable under accelerated conditions (40 °C/75% RH) and long-term conditions (25 °C/60% RH) for up to 1 year, with no differences in vesicle size and polydispersity when LAT was loaded. Nanotransfersomes increased the LAT cell proliferation effect in HaCaT cell via MAPK signaling pathway. Collectively, our results demonstrate LAT-nanotransfersomes formulation could be a promising therapy for hair growth disorders.

Nanodrug Delivery Strategies to Signaling Pathways in Alopecia

“…Lines 1 and 2 correspond to multichannel intensity plot profiles as a function of the depth (μm). Reproduced from ref . Copyright 2023 American Chemical Society.…”

“…Pena-Rodri ́guez et al 185 manufactured a latanoprost-loaded transferosome using ultrasonication and evaluated its ex vivo skin biodistribution in human scalp and porcine skin (Figure 4). The process involved homogenizing cholesterol, α-tocopherol, phosphatidylcholine, hydrogenated phosphatidylcholine, Tween 80, and latanoprost in an ethanol aqueous solution.…”