Late and prolonged post-training memory modulation in entorhinal and parietal cortex by drugs acting on the cAMP/protein kinase A signalling pathway

Ardenghi, Patrícia Grolli; Barros, Daniela M.; Izquierdo, Luciana A.; Bevilaqua, Lia R.; Schröder, Nadja; Quevedo, Joao L De; Rodrigues, C.; Vicente, Miguel Madruga; Medina, Jorge H.; Izquierdo, Iván

doi:10.1097/00008877-199712000-00010

Cited by 78 publications

(74 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One hundred and sixty animals (80 with cannulae in the hippocampus, 80 with cannulae in the entorhinal cortex) were trained in one-trial step-down inhibitory avoidance as described elsewhere (3,10,14,20,21,31). Briefly, the animals were placed on a 2.5-cm high and 7-cm wide platform facing a 43 x 25 cm grid of bronze bars spaced 1 cm apart.…”

Section: Methodsmentioning

confidence: 99%

“…Infusions were carried out over 30 s, after which the cannulae were left in place for another 15 s, first on one side and then on the other. Thus, the infusion procedure took slightly over 90 s (5,10,20,21). Infusions like these reach an area of approximately 1 mm 3 and diffuse away from the infusion site in less than 60 min (10,20,21,34) (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

“…The chain of events culminates at 3-6 h with a second peak of activation of the cAMP/PKA/P-CREB signaling pathway (6,8,19). Both PKA/P-CREB peaks are regulated by dopamine D 1 receptors (10,20,21). Some of these molecular steps also take place in the entorhinal cortex and other brain regions (6,20).…”

Section: Introductionmentioning

confidence: 99%

“…Both PKA/P-CREB peaks are regulated by dopamine D 1 receptors (10,20,21). Some of these molecular steps also take place in the entorhinal cortex and other brain regions (6,20).…”

Section: Introductionmentioning

confidence: 99%

“…The drugs studied were the PKA inhibitor Rp-cAMPs, the CaMKII inhibitor KN-62, the MAPKK inhibitor PD098059, the dopaminergic D 1 antagonist SCH23390, and the glutamatergic NMDA receptor antagonist 2-amino-5-phosphono pentanoic acid (AP5). The specific doses used here were chosen precisely because they had all been previously shown to have clear effects on memory of inhibitory avoidance (3,6,10,11,17,18,20,21) or other tasks (5,16) when infused into the hippocampus or the entorhinal cortex.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Pharmacological differences between memory consolidation of habituation to an open field and inhibitory avoidance learning

Vianna

Izquierdo

Barros

et al. 2001

Braz J Med Biol Res

View full text Add to dashboard Cite

Rats implanted bilaterally with cannulae in the CA1 region of the dorsal hippocampus or the entorhinal cortex were submitted to either a one-trial inhibitory avoidance task, or to 5 min of habituation to an open field. Immediately after training, they received intrahippocampal or intraentorhinal 0.5-µl infusions of saline, of a vehicle (2% dimethylsulfoxide in saline), of the glutamatergic N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-5-phosphono pentanoic acid (AP5), of the protein kinase A inhibitor Rp-cAMPs (0.5 µg/side), of the calcium-calmodulin protein kinase II inhibitor KN-62, of the dopaminergic D 1 antagonist SCH23390, or of the mitogen-activated protein kinase kinase inhibitor PD098059. Animals were tested in each task 24 h after training. Intrahippocampal KN-62 was amnestic for habituation; none of the other treatments had any effect on the retention of this task. In contrast, all of them strongly affected memory of the avoidance task. Intrahippocampal Rp-cAMPs, KN-62 and AP5, and intraentorhinal Rp-cAMPs, KN-62, PD098059 and SCH23390 caused retrograde amnesia. In view of the known actions of the treatments used, the present findings point to important biochemical differences in memory consolidation processes of the two tasks.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Both PKA/P-CREB peaks are regulated by dopamine D 1 receptors (10,20,21). Some of these molecular steps also take place in the entorhinal cortex and other brain regions (6,20).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pharmacological differences between memory consolidation of habituation to an open field and inhibitory avoidance learning

Vianna

Izquierdo

Barros

et al. 2001

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

Hippocampal levels of phosphorylated protein kinase a (phosphor‐S96) are linked to spatial memory enhancement by SGS742

Sunyer

Shim

et al. 2008

Hippocampus

View full text Add to dashboard Cite

Cognitive enhancement by the GABA (B) receptor antagonist SGS742 has been well-documented, but mechanisms of action are not fully elucidated. Previous work has proposed involvement of somatostatin-14 and protein kinase C in cognitive enhancement; phospho-protein kinase A (p-PKA), fyn, and phospho-fyn are known signaling systems for spatial memory. It was the aim of the study to determine hippocampal levels of these proteins following SGS742-treatment and to correlate them with the outcome from the Morris water maze (MWM), represented by the parameter "time spent in the target quadrant" during the probe trial. OF1 mice were used for the experiments and divided into four groups: intraperitoneal SGS742 and saline solution treatment, both, tested in the MWM, and two yoked controls. Six hours following the probe trial, hippocampal protein levels were determined by immunoblotting. In the MWM, time spent in the target quadrant was significantly enhanced by SGS742 treatment. p-PKA levels were significantly increased only in the SGS742-treated group tested in the MWM as compared to saline treatment. In yoked controls, no significant differences in p-PKA levels between SGS742 and saline treatment were observed. Somatostatin-14 levels were significantly increased in both SGS742-treated groups. No statistically significant changes of other protein levels were observed. We propose that GABA (B) antagonism represented by SGS742 treatment led to cognitive enhancement involving p-PKA, because yoked controls treated with SGS742 were comparable to yoked saline-treated controls. The finding that somatostatin-14 was also induced in the SGS742-treated yoked controls points to a drug side effect, and therefore the role of somatostatin-14 for cognitive enhancement remains open.

show abstract

Time-Dependent Impairment of Inhibitory Avoidance Retention in Rats by Posttraining Infusion of a Mitogen-Activated Protein Kinase Kinase Inhibitor into Cortical and Limbic Structures

Walz¹,

Roesler²,

Quevedo³

et al. 2000

Neurobiology of Learning and Memory

View full text Add to dashboard Cite

Late and prolonged post-training memory modulation in entorhinal and parietal cortex by drugs acting on the cAMP/protein kinase A signalling pathway

Cited by 78 publications

References 0 publications

Pharmacological differences between memory consolidation of habituation to an open field and inhibitory avoidance learning

Pharmacological differences between memory consolidation of habituation to an open field and inhibitory avoidance learning

Hippocampal levels of phosphorylated protein kinase a (phosphor‐S96) are linked to spatial memory enhancement by SGS742

Time-Dependent Impairment of Inhibitory Avoidance Retention in Rats by Posttraining Infusion of a Mitogen-Activated Protein Kinase Kinase Inhibitor into Cortical and Limbic Structures

Contact Info

Product

Resources

About