Late expression of red cell membrane protein 4.2 in normal human erythroid maturation with seven isoforms of the protein 4.2 gene

Absence of CD47 in protein 4.2-deficient hereditary spherocytosis in man: an interaction between the Rh complex and the band 3 complex

“…55 In contrast, protein 4.2 is expressed very late in erythroid maturation, after band 3 and protein 4.1, at a point suggested to be after the assembly of the cytoskeleton on the membrane. 56 Our results show that normal protein 4.2 mRNA levels are much reduced in the reticulocytes of the proband's mother. Surprisingly, although the red cells of the proband's mother have a normal complement of protein 4.2, these cells have CD47 content reduced to about half normal and also show altered glycosylation of RhAG.…”

Structure, Function, and Trafficking of SLC4 and SLC26 Anion Transporters

“…Early studies using cultured human erythroid precursors indicated a staged assembly process, whereby Protein 4.2 was the last protein to be assembled onto the cytoskeleton (Wada et al, 1999). Spectrin was detected early on, followed by ankyrin and Protein 4.1, then AE1, and finally Protein 4.2 (Wada et al, 1999;Wickrema, Koury, Dai, & Krantz, 1994).…”

“…Early studies using cultured human erythroid precursors indicated a staged assembly process, whereby Protein 4.2 was the last protein to be assembled onto the cytoskeleton (Wada et al, 1999). Spectrin was detected early on, followed by ankyrin and Protein 4.1, then AE1, and finally Protein 4.2 (Wada et al, 1999;Wickrema, Koury, Dai, & Krantz, 1994). Early work using chicken erythroblasts showed synthesis and assembly of the cytoskeletal proteins (spectrin, ankyrin, and Protein 4.1) well before AE1 (Cox, Stack, & Lazarides, 1987;Woods, Boyer, Vogt, & Lazarides, 1986).…”

A simple method for enrichment of polychromatic erythroblasts from rat bone marrow, and their proliferation and maturation in vitro

“…The collected cells were cultured following a cell culture method for human and mouse erythroblasts (Wada et al, 1999;Zhang et al, 2003) with some modifications. The culture medium consisted of Iscove's modified Dulbecco's medium (Invitrogen, Tokyo, Japan) supplemented with 20% heat-inactivated FBS, 100 U/ml penicillin (Wako Pure Chemical industries, Osaka, Japan), and 0.1 mg/ml streptomycin (Wako Pure Chemical industries).…”