Past vegetation and environmental changes and the role of climate as well as human activities are of major interest. The Hyrcanian region with a high number of endemic species has a poorly known paleoenvironmental history, especially the western region of northern Iran. This study aims to investigate a radiocarbon-dated sediment core of the Kholasht-Kouh Lake (KHL) for the recorded between ca. 510 and 1180 cal yr BP. KHL is located at 2000 m elevation and surrounded with steppe vegetation above the present-day forest line. Multi-proxy analyses including pollen, non-pollen palynomorphs, charcoal, loss on ignition, and X-ray fluorescence were applied to reconstruct past environmental dynamics. The results indicate the dominance of herbaceous vegetation (mainly Poaceae, Artemisia, and Amaranthaceae) around the lake during the whole record, except for the period between 1010 and 740 cal yr BP. During this period of the Medieval Climatic Anomaly (1000–700 cal yr BP) mixed forest increased, consisting mainly of Quercus, Fagus, Carpinus, and Juniperus. The K, Ti and Si elements (as indicators for increased in detrital inputs) suggest relatively humid condition between 1180 and 1010 cal yr BP. Furthermore, higher lake levels may indicate from low values of the Mn/Ti, Ca/Ti and Sr/Ti ratios. After 1010 until 740 cal yr BP the highest value of the Si/Fe ratio, suggested warmest period of the record. While the lowest value of the Si/Fe ratio, in line with the reduction of other elements and ratios, between 740 till 510 cal yr BP represented cold and dry period. Humans were present around the study area at least since beginning of the record, but did not played an important role impacting the vegetation.