Late-onset bilateral epithelial ingrowth following rapid corneal decompensation owing to amantadine

Gros-Louis, Philippe; Charest, Stephane; Legare, Marie E.

doi:10.1016/j.jcjo.2021.02.019

Cited by 4 publications

(1 citation statement)

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“…Cellular apoptosis and the functional development of organisms can be affected by amantadine [ 10 , 11 ]. At high amantadine concentrations in the human body, there is a risk of poisoning and tissue damage [ 12 , 13 , 14 ]. However, little is known about the effects of amantadine in aquatic products or the toxic effects of amantadine in A. japonicus [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics

Zhao

Chen

Tian

et al. 2023

Toxics

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Amantadine exposure can alter biological processes in sea cucumbers, which are an economically important seafood in China. In this study, amantadine toxicity in Apostichopus japonicus was analyzed by oxidative stress and histopathological methods. Quantitative tandem mass tag labeling was used to examine changes in protein contents and metabolic pathways in A. japonicus intestinal tissues after exposure to 100 µg/L amantadine for 96 h. Catalase activity significantly increased from days 1 to 3 of exposure, but it decreased on day 4. Superoxide dismutase and glutathione activities were inhibited throughout the exposure period. Malondialdehyde contents increased on days 1 and 4 but decreased on days 2 and 3. Proteomics analysis revealed 111 differentially expressed proteins in the intestines of A. japonicus after amantadine exposure compared with the control group. An analysis of the involved metabolic pathways showed that the glycolytic and glycogenic pathways may have increased energy production and conversion in A. japonicus after amantadine exposure. The NF-κB, TNF, and IL-17 pathways were likely induced by amantadine exposure, thereby activating NF-κB and triggering intestinal inflammation and apoptosis. Amino acid metabolism analysis showed that the leucine and isoleucine degradation pathways and the phenylalanine metabolic pathway inhibited protein synthesis and growth in A. japonicus. This study investigated the regulatory response mechanisms in A. japonicus intestinal tissues after exposure to amantadine, providing a theoretical basis for further research on amantadine toxicity.

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